Differential staining

Differential staining is a staining process which uses more than one chemical stain.[1] Using multiple stains can better differentiate between different microorganisms or structures/cellular components of a single organism.

Differential staining is used to detect abnormalities in the proportion of different white blood cells in the blood. The process or results are called a WBC differential. This test is useful because many diseases alter the proportion of certain white blood cells. By analyzing these differences in combination with a clinical exam and other lab tests, medical professionals can diagnose disease.

One commonly recognizable use of differential staining is the Gram stain. Gram staining uses two dyes: Crystal violet and Fuchsin or Safranin (the counterstain) to differentiate between Gram-positive bacteria (large Peptidoglycan layer on outer surface of cell) and Gram-negative bacteria.

Acid-fast stains are also differential stains.

## Further reading

* http://www.uphs.upenn.edu/bugdrug/antibiotic_manual/Gram2.htm The Gram Stain Technique

## References

1. ↑ Krueger, Woodrow B. (1986). Laboratory procedures for general microbiology. Kendall/Hunt Pub. Co. p. 25. ISBN 9780840338044. https://archive.org/details/laboratoryproced0000krue/page/24/mode/2up?q="Differential+staining". Retrieved 21 December 2021.

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