Ponceau S

Names
IUPAC name

tetrasodium;3-hydroxy-4-[[2-sulfonato-4-[(4-sulfonatophenyl)diazenyl]phenyl]diazenyl]naphthalene-2,7-disulfonate

Other names

Acid red 112

Identifiers

CAS Number

* 6226-79-5 Y

3D model (JSmol)

* Interactive image

ChEBI |

* CHEBI:90319 Y

ChemSpider |

* 11455708

EC Number |

* 228-319-2

PubChem CID

* 2723873

UNII |

* T1C2UGS05F Y

InChI

* Key: KQHKSGRIBYJYFX-UHFFFAOYSA-J

* InChI=1S/C22H16N4O13S4/c27-22-20(43(37,38)39)10-12-9-16(41(31,32)33)6-7-17(12)21(22)26-25-18-8-3-14(11-19(18)42(34,35)36)24-23-13-1-4-15(5-2-13)40(28,29)30/h1-11,27H,(H,28,29,30)(H,31,32,33)(H,34,35,36)(H,37,38,39)/b24-23+,26-25+

SMILES

* OC1=C(N=NC3=CC=C(N=NC4=CC=C(S(=O)(O)=O)C=C4)C=C3S(=O)(O)=O)C2=C(C=C(S(=O)(O)=O)C=C2)C=C1S(=O)(O)=O

Properties

Chemical formula

| C22H16N4O13S4
Molar mass | 672.63 g·mol−1
Hazards
GHS pictograms |
GHS Signal word | Warning

GHS hazard statements

| H315, H319, H335

GHS precautionary statements

| P261, P264, P271, P280, P302+352, P304+340, P305+351+338, P312, P321, P332+313, P337+313, P362, P403+233, P405, P501

Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).

Infobox references

Tracking categories (test):

* SizeSet

Ponceau S, Acid Red 112, or C.I. 27195 (systematic name: 3-hydroxy-4-(2-sulfo-4-[4-sulfophenylazo]phenylazo)-2,7-naphthalenedisulfonic acid sodium salt[1]) is a sodium salt of a diazo dye of a light red color, that may be used to prepare a stain for rapid reversible detection of protein bands on nitrocellulose or polyvinylidene fluoride (PVDF) membranes (western blotting), as well as on cellulose acetate membranes.[2] A Ponceau S stain is useful because it does not appear to have a deleterious effect on the sequencing of blotted polypeptides and is therefore one method of choice for locating polypeptides on western blots for blot-sequencing.[2] It is also easily reversed with water washes, facilitating subsequent immunological detection.[2] The stain can be completely removed from the protein bands by continued washing.[2] Common stain formulations include 0.1% (w/v) Ponceau S in 5% acetic acid or 2% (w/v) Ponceau S in 30% trichloroacetic acid and 30% sulfosalicylic acid.[3]

A nitrocellulose membrane stained with Ponceau S dye for protein detection during western blotting. The blue bands on the left are protein markers for various molecular weights.

## See also

* Coomassie brilliant blue
* Western blot normalization

## References

1. ↑ "Ponceau S solution". Sigma-Aldrich. http://www.sigmaaldrich.com/catalog/product/sial/p3504?lang=en. Retrieved 2016-11-24. S+solution&rft.atitle=&rft.pub=Sigma-Aldrich&rft_id=http://www.sigmaaldrich.com/catalog/product/sial/p3504?lang=en&rfr_id=info:sid/en.wikibooks.org:Chemistry:Ponceau_S">
2. ↑ 2.0 2.1 2.2 2.3 Al-Amoudi, M. S.; Salman, Mahmoud; Al-Majthoub, M. M.; Adam, Abdel Majid A.; Alshanbari, Naif A.; Refat, Moamen S. (9 October 2013). "Spectral studies to increase the efficiency and stability of laser dyes by charge-transfer reactions for using in solar cells: charge-transfer complexes of Ponceau S with p-chloranil, chloranilic and picric acids". Research on Chemical Intermediates 41 (5): 3089–3108. doi:10.1007/s11164-013-1417-4.
3. ↑ "Ponceau S solution". Sigma-Aldrich. http://www.sigmaaldrich.com/catalog/product/sial/p3504?lang=en. Retrieved 2016-11-24. "The following are two common stain formulations…" S+solution&rft.atitle=&rft.pub=Sigma-Aldrich&rft_id=http://www.sigmaaldrich.com/catalog/product/sial/p3504?lang=en&rfr_id=info:sid/en.wikibooks.org:Chemistry:Ponceau_S">

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*[CID]: Compound ID
*[H315]: Causes skin irritation
*[H319]: Causes serious eye irritation
*[H335]: May cause respiratory irritation
*[P261]: Avoid breathing dust/fume/gas/mist/vapours/spray
*[P264]: Wash … thoroughly after handling
*[P271]: Use only outdoors or in a well-ventilated area
*[P280]: Wear protective gloves/protective clothing/eye protection/face protection
*[P302+352]: IF ON SKIN: Wash with soap and water
*[P304+340]: IF INHALED: Remove victim to fresh air and keep at rest in a position comfortable for breathing
*[P305+351+338]: IF IN EYES: Rinse continuously with water for several minutes. Remove contact lenses if present and easy to do – continue rinsing
*[P312]: Call a POISON CENTER or doctor/physician if you feel unwell
*[P321]: Specific treatment (see … on this label)
*[P332+313]: If skin irritation occurs: Get medical advice/attention
*[P337+313]: Get medical advice/attention
*[P362]: Take off contaminated clothing and wash before reuse
*[P403+233]: Store in a well ventilated place. Keep container tightly closed
*[P405]: Store locked up
*[P501]: Dispose of contents/container to …