INT (chemical)

INT

Names
IUPAC name

3-(4-Iodophenyl)-2-(4-nitrophenyl)-5-phenyl-2H-tetrazol-3-ium chloride

Other names

2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride

Identifiers

CAS Number

* 146-68-9 Y

3D model (JSmol)

* Interactive image

ChemSpider |

* 58482

PubChem CID

* 629284

UNII |

* XY3JA5594E Y

InChI

* InChI=1S/C19H13IN5O2.ClH/c20-15-6-8-16(9-7-15)23-21-19(14-4-2-1-3-5-14)22-24(23)17-10-12-18(13-11-17)25(26)27;/h1-13H;1H/q+1;/p-1

Key: JORABGDXCIBAFL-UHFFFAOYSA-M

SMILES

* [Cl-].[O-][N+](=O)c1ccc(cc1)n3nc(n[n+]3c2ccc(I)cc2)c4ccccc4

Properties

Chemical formula

| C19H13ClIN5O2
Molar mass | 505.70 g·mol−1
Melting point |  240 °C (464 °F; 513 K) (decomposes)
Solubility in methanol: water (1:1)  | 50 mg/mL hot, very faintly turbid, very deep yellow
log P | −2.4

Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).

Infobox references

INT  (iodonitrotetrazolium or 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium) is a commonly used tetrazolium salt (usually prepared with chloride ions), similar to tetrazolium chloride that on reduction produces a red formazan dye that can be used for quantitative redox assays. It is also toxic to prokaryotes.[1]

INT is an artificial electron acceptor which can be utilized in a colorimetric assay to determine the concentration of protein in a solution. It can be reduced by succinate dehydrogenase to furazan, the formation of which can be measured by absorbance at 490 nm. The activity of succinate dehydrogenase is readily observed by the naked eye as the solution turns from colorless to rusty red.

## See also

* MTT assay

## References

1. ↑ Villegas-Mendoza, Josué; Cajal-Medrano, Ramón; Maske, Helmut (2015). "INT (2-(4-Iodophenyl)-3-(4-Nitrophenyl)-5-(Phenyl) Tetrazolium Chloride) Is Toxic to Prokaryote Cells Precluding Its Use with Whole Cells as a Proxy for In Vivo Respiration". Microbial Ecology 70 (4): 1004–1011. doi:10.1007/s00248-015-0626-3. PMID 25991603.

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*[CID]: Compound ID