Chronic myelomonocytic leukemia

Peripheral blood film of CMML. Monocytosis and the presence of myelocytes, metamyelocytes and promyelocytes is typical of CMML.
Specialty| Haematology, oncology
Causes| Environmental carcinogens, ionising radiation, cytotoxic agents
Diagnostic method| Blood film, genetic testing
Frequency| Less than 1 per 100,000 per year

Chronic myelomonocytic leukemia (CMML) is a type of leukemia, which are cancers of the blood-forming cells of the bone marrow. In adults, blood cells are formed in the bone marrow, by a process that is known as haematopoiesis. In CMML, there are increased numbers of monocytes and immature blood cells (blasts) in the peripheral blood and bone marrow, as well as abnormal looking cells (dysplasia) in at least one type of blood cell.[1]

CMML shows characteristics of a myelodysplastic syndrome (MDS); a disorder that produces abnormal looking blood cells, and a myeloproliferative neoplasm (MPN); a disorder characterised by the overproduction of blood cells. For this reason, CMML was reclassified as a MDS/MPN overlap disorder in 2002.[2] For a diagnosis of CMML, the World Health Organization (WHO) states that the blood monocyte count must be >1x109/L, no Philadelphia chromosome or mutations in the PDGFRA or PDGFRB gene should be present, the blast count must be <20% and dysplasia of at least one lineage of myeloid blood cell should be present.[3]

Azacitidine is a drug used to treat CMML and is approved by the Food and Drug Administration (FDA) and the European Medicines Agency. Stem cell transplant is also used to treat CMML, and involves the transplantation of donor haematopoietic stem cells into the recipient. Blood transfusion and erythropoietin are used to treat disease associated anaemia.[4][5][6]

## Contents

* 1 Signs and symptoms
* 2 Cause
* 3 Pathogenesis
* 3.1 Genetic mutations
* 4 Diagnosis
* 4.1 Classification
* 4.1.1 FAB classification
* 4.1.2 WHO classification
* 4.2 Diagnostic criteria
* 4.2.1 FAB criteria
* 4.2.2 WHO criteria
* 5 Treatment
* 6 Prognosis
* 6.1 Factors affecting prognosis
* 6.2 Scoring systems
* 6.2.1 IPSS
* 6.2.2 MD Anderson Prognostic Scoring System
* 6.2.3 The Düsseldorf score
* 7 Epidemiology
* 8 References
* 9 External links

## Signs and symptoms[edit | edit source]

One of the most common signs of CMML is splenomegaly, found in approximately half of cases. Other less frequent signs and symptoms consist of anaemia, fever, weight loss, night sweats, infection, bleeding, synovitis, lymphadenopathy, skin rashes, pleural effusion, pericardial effusion and peritoneal effusion.[7][8][9]

## Cause[edit | edit source]

Although the cause of CMML is unknown, environmental carcinogens, ionising radiation and cytotoxic agents may have a role in causing disease.[8] Approximately one third of cases of MDS with a monocyte count of >10% and <1x109/L will progress to CMML.[10]

## Pathogenesis[edit | edit source]

With a high rate of Ras mutation in CMML, deregulation of this signalling pathway has been linked to the pathogenesis of the disease. Tumour necrosis factor, GM-CSF, interleukin-3, interleukin-4, interleukin-6, and interleukin-10 may have a role in hyperproliferative CMML cells. These cytokines can stimulate the growth of CMML in vitro.[11] Hypermethylation of cytosine residues (usually in the promoter regions of genes) occurs in many malignancies to regulate gene expression. One commonly hypermethylated gene in CMML is p15INK4b, a gene involved in cell cycle regulation.[4]

### Genetic mutations[edit | edit source]

Clonal genetic abnormalities are common in CMML but they are not specific for diagnosis of the disease. The most common found are the 8+, −7/del (7q) and structural 12p abnormalities.[8] KRAS and NRAS are mutated in 25–40% of the cases of CMML. The Jak2 V617F mutation is found in 10% of cases. Mutations in transcription factors such as RUNX1, CEBPA, NPM1 and WT1 have been found in up to 30% of cases. Mutations of CBL are found in approximately 5–18% of cases.[3] Mutations in the TET2 gene are found in approximately 40–50% of CMML.[12] Inactivating mutations in one of the two parental GATA2 genes lead to a reduction, i.e. a haploinsufficiency, in the cellular levels of the gene's product, the GATA2 transcription factor, and thereby to a rare autosomal dominant genetic disease, GATA2 deficiency. This disease is associated with a highly variable set of disorders including the myelodysplastic syndrome, acute myeloid leukemia, and CMML. GATA2-deficiency-induced CMML, like other types of CMML, is commonly preceded by monocytosis.[13][14]

## Diagnosis[edit | edit source]

Bone marrow aspirate -finding was classified as CMML-2 (16% of myeloblasts)

Blood films display a range of abnormalities. A monocyte count of >1x109/L is essential for a diagnosis of CMML. Other features may include; leukocytosis (50% of cases); left shift and dysplasia of monocytes and granulocytes; presence of metamyelocytes, myelocytes and promonocytes; monocytes with hypersegmented/abnormal shaped nuclei, increased cytoplasmic basophilia and/or the presence of cytoplasmic granules; eosinophilia (in cases of CMML with eosinophilia); and spherocytosis (in cases of direct Coombs test, DCT, positive haemolytic anaemia). Platelet counts may be reduced, increased or normal.[7][15][16] Haemoglobin levels are usually reduced with normocytic and normochromic red blood cells. Autoantibodies and cold agglutinins may be present and 10% of CMML is DCT positive.[7][9] Bone marrow aspirates will display hypercellularity with increased counts of granulocytic and monocytic cells.[1] Bone marrow core biopsies may show a predominance of myelocytic and monocytic cells, abnormal localisation of immature precursors and dysplastic megakaryocytes.[1] Monocytic nodules are a common feature in biopsies.[16]

The phenotypical characteristics of CMML are; CD11b, CD11c, CD14, CD33, CD45 and CD64 seen in 100% of cases; CD13 found in 95% of cases; CD4 found in 76% of cases; HLA-DR found in 71% of cases; CD56 found in 53% of cases; CD2 found in 34% of cases; CD16 found in 29% of cases; CD10 found in 28% of cases; CD23 and CD7 found in 9% of cases; and CD117 found in 5% of cases.[17]

### Classification[edit | edit source]

Haematopoiesis. The two lineages of myeloid and lymphoid cells are formed from haematopoietic stem cells.

Leukemia subtypes are categorised into single clinical entities so that they can be diagnosed and treated appropriately. Leukaemias are subdivided into lymphoid and myeloid neoplasms, depending on which bone marrow cells are cancerous. The myeloid neoplasms contain acute and chronic leukemias, myelodysplastic syndromes (MDSs) and myeloproliferative neoplasms (MPNs). MPNs are characterised by increased production of myeloid blood cells, with a higher than normal number of mature cells. Unlike MPNs, MDSs have a dysfunctional production of myeloid cells with a reduced number of mature cells. Many of the cells produced in MDS are abnormal looking, known as dysplasia. CMML shows characteristics of both groups and thus is a difficult disease to categorise.[7][18]

#### FAB classification[edit | edit source]

The French-American-British (FAB) classification system was published in 1976 to classify the leukaemias. It placed CMML into the category of MDS, along with the refractory anaemia, refractory anaemia with ring sideroblasts, refractory anaemia with excess blasts and refractory anaemia with excess blasts in transformation. The system does have clinical utility; however factors such as cytogenetic status are not within the remit of the classification. For this reason, many disease entities in these groups show a great deal of heterogeneity.[18][19]

#### WHO classification[edit | edit source]

In 2001, the WHO Classification of Myeloid Neoplasms was published, classifying CMML into a new group of diseases, the myelodysplastic/myeloproliferative neoplasms (MDS/MPN), reflecting the disease's neoplastic nature. Other diseases in this category are juvenile myelomonocytic leukaemia, atypical CML; BCR-ABL1 negative and MDS/MPD unclassifiable. These MDS/MPN overlap syndromes have effective production of some lineages of blood cells, but show ineffective proliferation of other lineages. The 2008 revision of the classification moved cases of CMML with PDGFR gene translocations to a new group, myeloid/lymphoid neoplasms with eosinophilia with abnormalities of PDGFRA, PDGFRB or FGFR1.[2][7][20]

### Diagnostic criteria[edit | edit source]

#### FAB criteria[edit | edit source]

The FAB criteria for diagnosis are as follows:[21]

* Monocyte count >1x109/L
* 0–19% blasts in bone marrow
* <5% blasts in peripheral blood

The FAB also arbitrarily categorises CMML into myelodysplastic-like and myeloproliferative-like groups. A white blood count of 13x109 is used as a cut-off to differentiate the two.[12]

#### WHO criteria[edit | edit source]

The WHO criteria for diagnosis are as follows:[3]

* Persistent peripheral blood monocytosis with counts >1x109/L
* No Philadelphia chromosome or BCR-ABL1 fusion gene
* No rearrangement of PDGFRA or PDGFRB gene
* <20% myeloblasts, monoblasts and promonocytes in peripheral blood or bone marrow
* Dysplasia in one or more of the myeloid lineages; if myelodysplasia is absent or minimal then a diagnosis of CMML can be made if other requirements are met and:
* A molecular genetic abnormality is present in haematopoietic cells, or
* Monocytosis present for ≥3 months and other causes of monocytosis have been ruled out

WHO defined CMML has two main subsets, CMML-1 and CMML-2. CMML-1 is diagnosed if myeloblasts, monoblasts and promonocytes are <5% of peripheral blood and <10% of bone marrow. CMML-2 is diagnosed if:

* Myeloblasts, monoblasts or promonocytes are 5-19% in blood, or
* Myeloblasts, monoblasts or promonocytes are 10-19% in bone marrow, or
* Auer rods are present

CMML-1 and CMML-2 can be additionally grouped as CMML-1 or CMML-2 with eosinophilia. These are diagnosed if the above criteria are met and the blood eosinophil count is >1.5x109/L.[8]

Presence of two or more phenotypic abnormalities can aid a diagnosis of CMML in the absence of identifying cytogenetic or dysplastic features. These can include the expression of CD56 and/or CD2, or under-expression of HLA-DR.[3]

## Treatment[edit | edit source]

The treatment of CMML remains challenging due to the lack of clinical trials investigating the disease as its own clinical entity. It is often grouped with MDS in clinical trials, and for this reason the treatment of CMML is very similar to that of MDS. Most cases are dealt with as supportive rather than curative because most therapies do not effectively increase survival. Indications for treatment include the presence of B symptoms, symptomatic organ involvement, increasing blood counts, hyperleukocytosis, leukostasis and/or worsening cytopenias.[6][10]

Blood transfusions and erythropoietin administration are used to raise haemoglobin levels in cases with anemia.[6]

Azacitidine is a drug approved by the U.S. Food and Drug Administration (FDA) for the treatment of CMML and by the European Medicines Agency (EMA) for high risk non-proliferative CMML with 10–19% marrow blasts. It is a cytidine analogue that causes hypomethylation of DNA by inhibition of DNA methyltransferase. Decitabine is a similar drug to azacitidine and is approved by the FDA for treatments of all subtypes of MDS, including CMML. Hydroxyurea is a chemotherapy that is used in the myeloproliferative form of CMML to reduce cell numbers.[4][10][12] Decitabine/cedazuridine (Inqovi) is a fixed-dosed combination medication for the treatment of adults with myelodysplastic syndromes (MDS) and chronic myelomonocytic leukemia (CMML) that was approved for use in the United States in July 2020.[22]

Haematopoietic stem cell transplant remains the only curative treatment for CMML. However, due to the late age of onset and presence of other illnesses, this form of treatment is often not possible.[5][23]

## Prognosis[edit | edit source]

### Factors affecting prognosis[edit | edit source]

CMML-2 has a reduced overall survival as compared with CMML-1, with median survivals of 15 and 20 months, respectively. Myeloproliferative CMML (>13x109 monocytes/L) has a reduced survival compared with myelodysplastic CMML. A platelet count of <100 x109/L reduces overall survival. A haemoglobin level of <10g/dL has a reduced overall survival. Some cytogenetic abnormalities have implications on the prognosis of CMML. Normal karyotypes or the single loss of the Y chromosome have low risk prognoses. Trisomy 8, chromosome 7 abnormalities and complex karyotypes comprise a high risk group. Other cytogenetic abnormalities have intermediate prognoses. Somatic mutations in genes such as ASXL1 and EZH2 are associated with a poor prognosis.[12]

CMML has a 20–30% chance of transformation to AML, a lower rate than other similar diseases. The CMML-2 subtype is associated with increased risk of transformation and ASXL1 and RUNX1 mutations also increase the risk of transition to AML.[12][24][25]

### Scoring systems[edit | edit source]

#### IPSS[edit | edit source]

The International Prognostic Scoring System (IPSS) was developed in the mid-1990s to assess the prognosis of MDS patients. This system stratifies cases into 2 groups; a lower-risk group (sub divided into low and intermediate-1) and a higher risk (subdivided into intermediate-2 and high). It uses the blast percentage, number of cytopenias and bone marrow cytogenetics data to place cases of CMML into these groups. Due to the scoring system being developed for MDS, the more myeloproliferative cases of CMML (WBC >13x109) are excluded from the scoring system. Although the IPSS scoring system is used clinically, there is a high variability in each group. For this reason, new modalities for assessing prognosis in MDS (and CMML) are being developed.[12][26]

#### MD Anderson Prognostic Scoring System[edit | edit source]

A new method developed using data from the M.D. Anderson Cancer Center found that a haemoglobin level of <12g/dL, total circulating lymphocyte count of >2.5 x 109/L, >0% immature myeloid cells, >10% bone marrow blasts causes a reduced overall survival. This data allows cases of CMML to be stratified into low, intermediate-1, intermediate-2 and high risk groups. These groups have median survival times of 24, 15, 8 and 5 months respectively.[27][28]

#### The Düsseldorf score[edit | edit source]

The Düsseldorf score stratifies cases using four categories, giving one point for each; bone marrow blasts ≥5%, LDH >200U/L, haemoglobin ≤9g/dL and a platelet count ≤100,000/uL. A score of 0 indicates a low risk group' 1-2 indicates an intermediate risk group and 3-4 indicates a high risk group. The cumulative 2 year survival of scores 0, 1-2 and 3-4 is 91%, 52% and 9%; and risk of AML transformation is 0%, 19% and 54% respectively.[10]

## Epidemiology[edit | edit source]

There have been few individual epidemiological studies of CMML, due to the difficulty in the disease classification. CMML has an estimated incidence of less than 1 per 100,000 persons per year.[12] The median age of diagnosis is 65–75. CMML has a propensity for males rather than females, at a ratio of 1.5–3:1.[8]

## References[edit | edit source]

1. ↑ 1.0 1.1 1.2 Foucar K (August 2009). "Myelodysplastic/myeloproliferative neoplasms". Am. J. Clin. Pathol. 132 (2): 281–9. doi:10.1309/AJCPJ71PTVIKGEVT. PMID 19605822.
2. ↑ 2.0 2.1 Vardiman JW, Harris NL, Brunning RD (October 2002). "The World Health Organization (WHO) classification of the myeloid neoplasms". Blood. 100 (7): 2292–302. doi:10.1182/blood-2002-04-1199. PMID 12239137.
3. ↑ 3.0 3.1 3.2 3.3 Vardiman J, Hyjek E (2011). "World health organization classification, evaluation, and genetics of the myeloproliferative neoplasm variants". Hematology. 2011: 250–6. doi:10.1182/asheducation-2011.1.250. PMID 22160042.
4. ↑ 4.0 4.1 4.2 McCormack, SE; Warlick, ED (Sep 7, 2010). "Epigenetic approaches in the treatment of myelodysplastic syndromes: clinical utility of azacitidine". OncoTargets and Therapy. 3: 157–65. doi:10.2147/OTT.S5852. PMC 2939768. PMID 20856790.
5. ↑ 5.0 5.1 Robert J. Soiffer (17 November 2008). Hematopoietic Stem Cell Transplantation. Springer. ISBN 978-1-934115-05-3. Archived from the original on 28 June 2014. Retrieved 23 September 2012.
6. ↑ 6.0 6.1 6.2 Bennett, JM (June 2002). "Chronic myelomonocytic leukemia". Current Treatment Options in Oncology. 3 (3): 221–3. doi:10.1007/s11864-002-0011-6. PMID 12057067.
7. ↑ 7.0 7.1 7.2 7.3 7.4 Bain, Barbara J. (2003). Leukaemia Diagnosis. Cambridge, MA: Blackwell Publishers. ISBN 978-1-4051-0661-0.
8. ↑ 8.0 8.1 8.2 8.3 8.4 Pathology and Genetics of Haemo (World Health Organization Classification of Tumours S.). Oxford Univ Pr. 2003. ISBN 978-92-832-2411-2.
9. ↑ 9.0 9.1 Paul Moss; Victor Hoffbrand (2011). Essential Haematology, Includes FREE Desktop Edition (Essentials). Wiley-Blackwell. ISBN 978-1-4051-9890-5.
10. ↑ 10.0 10.1 10.2 10.3 Viktoria Faber; Richard Greil; Lisa Pleyer; Daniel Neureiter (2010). Chronic Myeloid Neoplasias and Clonal Overlap Syndromes: Epidemiology, Pathophysiology and Treatment Options. Berlin: Springer. ISBN 978-3-211-79891-1.
11. ↑ Arceci RJ, Longley BJ, Emanuel PD (2002). "Atypical cellular disorders". Hematology. 2002: 297–314. doi:10.1182/asheducation-2002.1.297. PMID 12446429.
12. ↑ 12.0 12.1 12.2 12.3 12.4 12.5 12.6 Cazzola M, Malcovati L, Invernizzi R (2011). "Myelodysplastic/myeloproliferative neoplasms". Hematology. 2011: 264–72. doi:10.1182/asheducation-2011.1.264. PMID 22160044. S2CID 24489846.
13. ↑ Crispino JD, Horwitz MS (April 2017). "GATA factor mutations in hematologic disease". Blood. 129 (15): 2103–2110. doi:10.1182/blood-2016-09-687889. PMC 5391620. PMID 28179280.
14. ↑ Hirabayashi S, Wlodarski MW, Kozyra E, Niemeyer CM (August 2017). "Heterogeneity of GATA2-related myeloid neoplasms". International Journal of Hematology. 106 (2): 175–182. doi:10.1007/s12185-017-2285-2. PMID 28643018.
15. ↑ Hirschmann, Jan V.; Tkachuk, Douglas C.; Wintrobe, Maxwell Myer (2007). Wintrobe's atlas of clinical hematology. Philadelphia: Wolters Kluwer Health/Lippincott Williams & Wilkins. ISBN 978-0-7817-7023-1.
16. ↑ 16.0 16.1 Wayne W. Grody; Naeim, Faramarz (2008). Hematopathology: morphology, immunophenotype, cytogenetics, and molecular approaches. Amsterdam: Elsevier/Academic Press. ISBN 978-0-12-370607-2.
17. ↑ Foxwell Nathan Emmons; Wojciech Gorczyca; James Weisberger (2004). An Atlas of Differential Diagnosis in Neoplastic Hematopathology. Washington, DC: Taylor & Francis. ISBN 978-1-84214-247-9.
18. ↑ 18.0 18.1 Bhargava R, Dalal BI (2010). "Two steps forward, one step back: 4th WHO classification of myeloid neoplasms (2008)". Indian J Pathol Microbiol. 53 (3): 391–4. doi:10.4103/0377-4929.68240. PMID 20699489.
19. ↑ Turgeon, Mary Louise (1999). Clinical hematology: theory and procedures. Hagerstwon, MD: Lippincott Williams & Wilkins. pp. 321–322. ISBN 978-0-316-85623-2.
20. ↑ Tsongalis, Gregory J.; Coleman, William L. (2009). MOLECULAR PATHOLOGY: THE MOLECULAR BASIS OF HUMAN DISEASE; ED. BY WILLIAM B. COLEMAN. Amsterdam: Elsevier Academic Press. ISBN 978-0-12-374419-7.
21. ↑ Bennett JM, Catovsky D, Daniel MT, et al. (June 1982). "Proposals for the classification of the myelodysplastic syndromes". Br. J. Haematol. 51 (2): 189–99. CiteSeerX 10.1.1.630.8355. doi:10.1111/j.1365-2141.1982.tb02771.x. PMID 6952920.
22. ↑ "FDA Approves New Therapy for Myelodysplastic Syndromes (MDS) That Can Be Taken at Home". U.S. Food and Drug Administration (FDA) (Press release). 7 July 2020. Archived from the original on 7 July 2020. Retrieved 7 July 2020. This article incorporates text from this source, which is in the public domain.
23. ↑ Bacher U, Haferlach T, Schnittger S, Kreipe H, Kröger N (March 2011). "Recent advances in diagnosis, molecular pathology and therapy of chronic myelomonocytic leukaemia". Br J Haematol. 153 (2): 149–67. doi:10.1111/j.1365-2141.2011.08631.x. PMID 21401573.
24. ↑ William G. Finn; LoAnn C. Peterson (31 May 2004). Hematopathology in Oncology. Springer. pp. 33–. ISBN 978-1-4020-7919-1. Archived from the original on 15 February 2017. Retrieved 23 September 2012.
25. ↑ Barbara J. Bain (2003). Chronic Myeloproliferative Disorders: Cytogenetic and Molecular Genetic Abnormalities. Karger Publishers. pp. 72–. ISBN 978-3-8055-7307-8. Archived from the original on 15 February 2017. Retrieved 23 September 2012.
26. ↑ Greenberg P, Cox C, LeBeau MM, et al. (March 1997). "International scoring system for evaluating prognosis in myelodysplastic syndromes". Blood. 89 (6): 2079–88. doi:10.1182/blood.V89.6.2079. PMID 9058730.
27. ↑ Garcia-Manero G (2010). "Prognosis of myelodysplastic syndromes". Hematology. 2010: 330–7. doi:10.1182/asheducation-2010.1.330. PMID 21239815.
28. ↑ Onida F, Kantarjian HM, Smith TL, et al. (February 2002). "Prognostic factors and scoring systems in chronic myelomonocytic leukemia: a retrospective analysis of 213 patients". Blood. 99 (3): 840–9. doi:10.1182/blood.V99.3.840. PMID 11806985. S2CID 1310629.

## External links[edit | edit source]

Classification|

* ICD-10-CM: C93.1
* ICD-9-CM: 205.1
* ICD-O: M9945/3
* MeSH: D015477

|

External resources|

* Orphanet: 98823

* v
* t
* e

Myeloid-related hematological malignancy

CFU-GM/
and other granulocytes|

| CFU-GM|

| Myelocyte|

| AML:|

* Acute myeloblastic leukemia
* M0
* M1
* M2
* APL/M3

|

MP|

* Chronic neutrophilic leukemia

Monocyte|

| AML|

* AMoL/M5
* Myeloid dendritic cell leukemia

|

CML|

* Philadelphia chromosome
* Accelerated phase chronic myelogenous leukemia

Myelomonocyte|

| AML|

* M4

|

MD-MP|

* Juvenile myelomonocytic leukemia
* Chronic myelomonocytic leukemia

Other|

* Histiocytosis

CFU-Baso|

| AML|

* Acute basophilic

|

CFU-Eos|

| AML|

* Acute eosinophilic

|

MP|

* Chronic eosinophilic leukemia/Hypereosinophilic syndrome

MEP|

| CFU-Meg|

| MP|

* Essential thrombocythemia

* Acute megakaryoblastic leukemia

|

CFU-E|

| AML|

* Erythroleukemia/M6

|

MP|

* Polycythemia vera

MD|

* Refractory anemia
* Refractory anemia with excess of blasts
* Chromosome 5q deletion syndrome
* Sideroblastic anemia
* Paroxysmal nocturnal hemoglobinuria
* Refractory cytopenia with multilineage dysplasia

CFU-Mast|

| Mastocytoma|

* Mast cell leukemia
* Mast cell sarcoma
* Systemic mastocytosis

|

Mastocytosis:|

* Diffuse cutaneous mastocytosis
* Erythrodermic mastocytosis
* Adult type of generalized eruption of cutaneous mastocytosis
* Urticaria pigmentosa
* Mast cell sarcoma
* Solitary mastocytoma

Systemic mastocytosis|

* Xanthelasmoidal mastocytosis

Multiple/unknown|

| AML|

* Acute panmyelosis with myelofibrosis
* Myeloid sarcoma

|

MP|

* Primary myelofibrosis
* Biphenotypic acute leukaemia

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