CyaA

Bifunctional hemolysin/adenylate cyclase

Schematic representation of CyaA.

Identifiers
Organism| Bordetella pertussis
Symbol| cya
Alt. symbols| cyaA
Entrez| 45387797
RefSeq (Prot)| WP_010929995.1
UniProt| P0DKX7
Other data
EC number| 4.6.1.1
Chromosome| genome: 0.49 - 0.5 Mb
| Search for

Structures| Swiss-model
Domains| InterPro

Adenylate cyclase toxin (CyaA) is released from bacterium Bordetella pertussis by the T1SS (Type 1 secretion system) and released in the host’s respiratory tract in order to suppress its early innate and subsequent adaptive immune defense.[1]

CyaA plays a particular role in the early phases of airway colonization. It is able to instantly ablate the bactericidal oxidative burst, along with the opsonophagocytic killing capacities of neutrophils and macrophages. As a result, it enables establishment of Bordetella infection of airway mucosa and promotes immune evasion of B. pertussis, by affecting the host’s immune cells.[1]

## Contents

* 1 Structure
* 2 Mode of action in host cells
* 3 Interference with immune defence
* 4 See also
* 5 References

## Structure

The toxin is a 1706 residue-long polypeptide that consists of an N-terminal ~400 residue-long adenylate cyclase (AC) enzyme that is linked to a characteristic RTX hemolysin (Hly) moiety of ~1300 residues. This Hly moiety itself consists of four functional subdomains, comprising: (i) a hydrophobic pore-forming domain ; (ii) an activation domain, with the two posttranslationally acylated lysine residues; (iii) a receptor-binding RTX domain consisting of ~40 typical calcium-binding RTX nonapeptide repeats]; and (iv) a non-processed C-terminal secretion signal recognized by a bacterial type I secretion system (T1SS), respectively.[2]

## Mode of action in host cells

Once in host cells, CyaA binds the complement receptor 3 (CR3), the αMβ2 integrin known also as CD11b/CD18 or Mac-1. Then, the toxin translocates its AC enzyme domain across the cytoplasmic membrane of CR3-expressing myeloid cells, such as macrophages, neutrophils and dendritic cells.[1]

Mode of action of adenylate cyclase toxin (CyaA) on phagocyte membrane.

Inserting in the cell’s membrane results in an influx of calcium ions that leads to a calpain-mediated cleavage of talin. The CyaA–CR3 complex relocates into lipid rafts and the AC domain is translocated across the cellular membrane into the transmembrane region, where signaling complexes, such as protein kinase A, are clustered.[3]

Inside the cells, the AC enzyme binds calmodulin and catalyzes unregulated conversion of ATP into the key second messenger molecule 3',5'-cyclic adenosine monophosphate (cAMP). cAMP is responsible for the incapacitation of the bactericidal activities of the target cells. The RTX hemolysin part of CyaA is functionally independent of AC domain and forms oligomeric cation-selective pores that allow cytosolic potassium ions to leak from cells via the cellular membrane.[3]

## Interference with immune defence

The fast production of very high levels of intracellular cAMP in CD11b-expressing immune cells that encounter CyaA, immediately interferes with the physiological functions of phagocytes.[1] In monocytes and macrophages, the CyaA-produced cAMP signaling through the cAMP/protein kinase A (PKA) pathway blocks the production of reactive oxygen species (ROS). This also halts the complement-mediated uptake of opsonized particles. At higher CyaA doses, FcR-mediated phagocytosis is also inhibited.[4]

Through cAMP signaling, CyaA also alters TLR-triggered maturation of dendritic cells, inhibiting proinflammatory IL-12 and TNF-α secretion and enhancing IL-10 production and Treg expansion, preventing the induction of adaptive immune responses to Bordetella infections.[5]

The enzymatic AC activity of CyaA also seems to prolong the intracellular survival of non-opsonized internalized (invading) B. pertussis bacteria that enter into human and murine macrophages by a non-phagocytic mechanism.[4]

## See also

* Pertussis toxin
* Anthrax toxin

## References

1. ↑ 1.0 1.1 1.2 1.3 "Invasion of Dendritic Cells, Macrophages and Neutrophils by the Bordetella Adenylate Cyclase Toxin: A Subversive Move to Fool Host Immunity". Toxins 9 (10): 293. September 2017. doi:10.3390/toxins9100293. PMID 28934122.
2. ↑ "Structure-Function Relationships Underlying the Capacity of Bordetella Adenylate Cyclase Toxin to Disarm Host Phagocytes". Toxins 9 (10): 300. September 2017. doi:10.3390/toxins9100300. PMID 28946636.
3. ↑ 3.0 3.1 "Structure-Function Relationships Underlying the Capacity of Bordetella Adenylate Cyclase Toxin to Disarm Host Phagocytes". PLOS Pathogens 6 (5): e1000901. May 2010. doi:10.1371/journal.ppat.1000901. PMID 20485565.
4. ↑ 4.0 4.1 "Adenylate cyclase toxin subverts phagocyte function by RhoA inhibition and unproductive ruffling". Journal of Immunology 181 (8): 5587–97. October 2008. doi:10.4049/jimmunol.181.8.5587. PMID 18832717.
5. ↑ "Adenylate cyclase toxin-hemolysin relevance for pertussis vaccines". Expert Review of Vaccines 13 (10): 1215–27. October 2014. doi:10.1586/14760584.2014.944900. PMID 25090574.

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Phosphorus–oxygen lyases (EC 4.6)

4.6.1|

| Adenylate cyclase
(4.6.1.1)|

| intracellular|

* ADCY1
* ADCY2
* ADCY3
* ADCY4
* ADCY5
* ADCY6
* ADCY7
* ADCY8
* ADCY9
* ADCY10

|

extracellular|

* Extracellular adenylate cyclase

Guanylate cyclase
(4.6.1.2)|

| soluble guanylyl cyclase|

* GUCY1A2
* GUCY1A3
* GUCY1B2
* GUCY1B3

|

guanylate cyclase-coupled receptor|

* GUCY2C
* GUCY2D
* ANP (NPR1
* NPR2)

Other|

* Cytidylate cyclase
* 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
* Phosphatidylinositol diacylglycerol-lyase
* Glycosylphosphatidylinositol diacylglycerol-lyase
* FAD-AMP lyase (cyclizing)

* v
* t
* e

Enzymes

Activity|

* Active site
* Binding site
* Catalytic triad
* Oxyanion hole
* Enzyme promiscuity
* Catalytically perfect enzyme
* Coenzyme
* Cofactor
* Enzyme catalysis

Regulation|

* Allosteric regulation
* Cooperativity
* Enzyme inhibitor
* Enzyme activator

Classification|

* EC number
* Enzyme superfamily
* Enzyme family
* List of enzymes

Kinetics|

* Enzyme kinetics
* Eadie–Hofstee diagram
* Hanes–Woolf plot
* Lineweaver–Burk plot
* Michaelis–Menten kinetics

Types|

* EC1 Oxidoreductases (list)
* EC2 Transferases (list)
* EC3 Hydrolases (list)
* EC4 Lyases (list)
* EC5 Isomerases (list)
* EC6 Ligases (list)
* EC7 Translocases (list)

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