Sudan IV

Names
Preferred IUPAC name

1-[{2-Methyl-4-[(2-methylphenyl)diazenyl]phenyl}diazenyl]naphthalen-2-ol

Other names

1-{2-Methyl-4-[(2-methylphenyl)diazenyl]phenyl}azonaphthalen-2-ol
Sudan R, C.I. Solvent Red 24, C.I. 26105, Lipid Crimson, Oil Red, Oil Red BB, Fat Red B, Oil Red IV, Scarlet Red, Scarlet Red N.F, Scarlet Red Scharlach, Scarlet R

Identifiers

CAS Number

* 85-83-6 N

3D model (JSmol)

* Interactive image

ChEBI |

* CHEBI:88014 Y

ChEMBL |

* ChEMBL1707454

ChemSpider |

* 11252033 Y

EC Number |

* 201-635-8

KEGG |

* C19520 Y

PubChem CID

* 62330

UNII |

* I35E9QU96C

InChI

* InChI=1S/C24H20N4O/c1-16-7-3-6-10-21(16)26-25-19-12-13-22(17(2)15-19)27-28-24-20-9-5-4-8-18(20)11-14-23(24)29/h3-15,29H,1-2H3/b26-25+,28-27+ Y

Key: RCTGMCJBQGBLKT-PAMTUDGESA-N Y

* InChI=1S/C24H20N4O/c1-16-7-3-6-10-21(16)26-25-19-12-13-22(17(2)15-19)27-28-24-20-9-5-4-8-18(20)11-14-23(24)29/h3-15,29H,1-2H3/b26-25+,28-27+

Key: RCTGMCJBQGBLKT-PAMTUDGEBE

* Key: RCTGMCJBQGBLKT-PAMTUDGESA-N

SMILES

* Cc4ccccc4/N=N/c3ccc(/N=N/c1c2ccccc2ccc1O)c(C)c3

Properties

Chemical formula

| C24H20N4O
Hazards
GHS pictograms |
GHS Signal word | Warning

GHS hazard statements

| H315, H319

GHS precautionary statements

| P264, P280, P302+352, P305+351+338, P321, P332+313, P337+313, P362

Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).

N verify (what is YN ?)
Infobox references

Tracking categories (test):

* SizeSet

Sudan IV (C24H20N4O) is a lysochrome (fat-soluble dye) diazo dye used for the staining of lipids, triglycerides and lipoproteins on frozen paraffin sections. It has the appearance of reddish brown crystals with melting point 199 °C and maximum absorption at 520(357) nm.

Sudan IV is one of the dyes used for Sudan staining. Similar dyes include Oil Red O, Sudan III, and Sudan Black B. Staining is an important biochemical technique, offering the ability to visually qualify the presence of the fatty compound of interest without isolating it. For staining purposes, Sudan IV can be made up in propylene glycol.[1] Alternatively, authors have reported using the dye saturated in isopropyl alcohol, 95% ethanol, or 0.05% by weight in acetone:ethanol:water (50:35:15)[citation needed]. The idea is to use a moderately apolar solvent to solubilize the dye allowing it to partition into the highly apolar fat without the solvent solubilizing the fat to be stained.

Sudan I, Sudan III, and Sudan IV have been classified as category 3 carcinogen by the International Agency for Research on Cancer.[2]

In its purified form it is called Biebrich scarlet R, which should not be confused with the water-soluble Biebrich scarlet.

In industry, it is used to color nonpolar substances like oils, fats, waxes, greases, various hydrocarbon products, and acrylic emulsions. Sudan IV is also used in United Kingdom as a fuel dye to dye lower-taxed heating oil; because of that it is also known as Oil Tax Red. As a food dye, Sudan IV is considered an illegal dye, mainly because of its harmful effect over a long period of time, as it is a carcinogen. It was ruled unsafe in the 1995 food safety regulations report.

While the dye does stain triglycerides with an intense red color, it does not stain lipid droplets or phospholipids. Sudan IV can be used to stain the aorta by staining lipid-rich plaques. The dye can also be used to stain lipids in atherosclerotic lesions in mice.[3]

## References

1. ↑ "Oil Red O Staining Protocol". http://www.ihcworld.com/_protocols/special_stains/oil_red_o.htm. Retrieved 2021-03-17.
2. ↑ "The induction of cytochrome P450 1A1 by sudan dyes". J. Biochem. Mol. Toxicol. 22 (2): 77–84. 2008. doi:10.1002/jbt.20220. PMID 18418879.
3. ↑ "Sudan IV". https://www.sigmaaldrich.com/US/en/product/aldrich/198102.

## External links

* Stains File entry

* v
* t
* e

Microbial and histological stains

Iron/hemosiderin|

* Perls' Prussian blue

Lipids|

* Sudan stain
* Sudan II
* Sudan III
* Sudan IV
* Oil Red O
* Sudan Black B

Carbohydrates|

* Periodic acid–Schiff stain

Amyloid|

* Congo red

Bacteria|

* Gram stain
* Methyl violet/Gentian violet
* Safranin
* Ziehl–Neelsen stain/acid-fast
* Carbol fuchsin/Fuchsine
* Methylene blue
* Auramine–rhodamine stain
* Auramine O
* Rhodamine B

Connective tissue|

* trichrome stain: Masson's trichrome stain/Lillie's trichrome
* Light Green SF yellowish
* Biebrich scarlet
* Phosphomolybdic acid
* Fast Green FCF

* Van Gieson's stain

Other|

* Alcian blue stain
* Cyanine
* Jaswant Singh–Bhattacharji (JSB) stain
* H&E stain
* Haematoxylin
* Eosin Y
* Janus Green B
* Giemsa stain
* Gömöri trichrome stain
* Luxol fast blue stain
* Methyl blue
* Movat's stain
* Neutral red
* Silver stain
* Bielschowsky stain
* Grocott's methenamine silver stain
* Warthin–Starry stain
* Wright's stain

Tissue stainability|

* Acidophilic
* Basophilic
* Chromophobic

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*[CID]: Compound ID
*[H315]: Causes skin irritation
*[H319]: Causes serious eye irritation
*[P264]: Wash … thoroughly after handling
*[P280]: Wear protective gloves/protective clothing/eye protection/face protection
*[P302+352]: IF ON SKIN: Wash with soap and water
*[P305+351+338]: IF IN EYES: Rinse continuously with water for several minutes. Remove contact lenses if present and easy to do – continue rinsing
*[P321]: Specific treatment (see … on this label)
*[P332+313]: If skin irritation occurs: Get medical advice/attention
*[P337+313]: Get medical advice/attention
*[P362]: Take off contaminated clothing and wash before reuse
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