Wet chemistry

Short description: Form of analytical chemistry

Graduated cylinders and beakers filled with chemicals

Wet chemistry is a form of analytical chemistry that uses classical methods such as observation to analyze materials. It is called wet chemistry since most analyzing is done in the liquid phase.[1] Wet chemistry is also called bench chemistry since many tests are performed at lab benches.[2]

## Contents

* 1 Materials
* 2 History
* 3 Methods
* 3.1 Qualitative methods
* 3.1.1 Chemical tests
* 3.1.1.1 Flame test
* 3.2 Quantitative methods
* 3.2.1 Gravimetric analysis
* 3.2.2 Volumetric analysis
* 3.3 Colorimetry
* 4 Uses
* 5 See also
* 6 Further reading
* 7 References

## Materials

Wet chemistry commonly uses laboratory glassware such as beakers and graduated cylinders to prevent materials from being contaminated or interfered with by unintended sources.[3] Gasoline, Bunsen burners, and crucibles may also be used to evaporate and isolate substances in their dry forms.[4][5] Wet chemistry is not performed with any advanced instruments since most automatically scan substances.[6] Although, simple instruments such as scales are used to measure the weight of a substance before and after a change occurs.[7] Many high school and college laboratories teach students basic wet chemistry methods.[8]

## History

Before the age of theoretical and computational chemistry, wet chemistry was the predominant form of scientific discovery in the chemical field. This is why it is sometimes referred to as classic chemistry or classical chemistry. Scientists would continuously develop techniques to improve the accuracy of wet chemistry. Later on, instruments were developed to conduct research impossible for wet chemistry. Over time, this became a separate branch of analytical chemistry called instrumental analysis. Because of the high volume of wet chemistry that must be done in today's society and new quality control requirements, many wet chemistry methods have been automated and computerized for streamlined analysis. The manual performance of wet chemistry mostly occurs in schools.

## Methods

### Qualitative methods

Qualitative methods use changes in information that cannot be quantified to detect a change. This can include a change in color, smell, texture, etc.[9][10]

#### Chemical tests

When burned, lead produces a bright white flame.

Chemical tests use reagents to indicate the presence of a specific chemical in an unknown solution. The reagents cause a unique reaction to occur based on the chemical it reacts with, allowing one to know what chemical is in the solution. An example is Heller's test where a test tube containing proteins has strong acids added to it. A cloudy ring forms where the substances meet, indicating the acids are denaturing the proteins. The cloud is a sign that proteins are present in a liquid. The method is used to detect proteins in a person's urine.

##### Flame test

The flame test is a more well known version of the chemical test. It is only used on metallic ions. The metal powder is burned, causing an emission of colors based on what metal was burned. For example, calcium (Ca) will burn orange and copper (Cu) will burn blue. Their color emissions are used to produce bright colors in fireworks.

### Quantitative methods

Quantitative methods use information that can be measured and quantified to indicate a change. This can include changes in volume, concentration, weight, etc.

#### Gravimetric analysis

Solids are filtered out of the liquid, which is collected in the beaker.

Gravimetric analysis measures the weight or concentration of a solid that has either formed from a precipitate or dissolved in a liquid. The mass of the liquid is recorded before undergoing the reaction. For the precipitate, a reagent is added until the precipitate stops forming. The precipitate is then dried and weighed to determine the chemicals concentration in the liquid. For a dissolved substance, the liquid can be filtered until the solids are removed or boiled until all the liquid evaporates. The solids are left alone until completely dried and then weighed to determine its concentration. Evaporating all the liquid is the more common approach.

#### Volumetric analysis

Titration is called volumetric analysis since it relies on volume measurements to determine the quantity of a chemical. A reagent with a known volume and concentration is added to a solution with an unknown substance and concentration. The amount of reagent required for a change to occur is proportional to the amount of the unknown substances. This reveals the amount of the unknown substance present. If no visible change is present, an indicator is added to the solution. For example, a pH indicator changes color based on the pH of the solution. The exact point where the color change occurs is called the endpoint. Since the color change can occur very suddenly, it is important to be extremely precise with all measurements.

### Colorimetry

Colorimetry is a unique method since it has both qualitative and quantitative properties. Its qualitative analysis involves recording color changes to indicate a change has occurred. This can be a change in shading of the color or a change into a completely different color. The quantitative aspect involves sensory equipment that can measure the wavelength of colors. Changes in wavelengths can be precisely measured and help indicate changes.

## Uses

Wet chemistry techniques can be used for qualitative chemical measurements, such as changes in color (colorimetry), but often involves more quantitative chemical measurements, using methods such as gravimetry and titrimetry. Some uses for wet chemistry include tests for:

* pH (acidity, alkalinity)
* concentration
* conductivity (specific conductance)
* cloud point (nonionic surfactants)
* hardness
* melting point
* solids or dissolved solids
* salinity
* specific gravity
* density
* turbidity
* viscosity
* moisture (Karl Fischer titration)

Wet chemistry is also used in environmental chemistry settings to determine the current state of the environment. It is used to test:

* Biochemical Oxygen Demand (BOD)
* Chemical Oxygen Demand (COD)
* eutrophication
* coating identification

It can also involve the elemental analysis of samples, e.g., water sources, for items like:

* Ammonia nitrogen
* Chloride
* Chromium
* Cyanide
* Dissolved oxygen
* Fluoride
* Nitrogen
* Nitrate
* Phenols
* Phosphate
* Phosphorus
* Silica
* Sulfate, sulfide

## See also

* Wet laboratory

## Further reading

* Elizabeth K. Wilson. "Phoenix Tastes Water Ice On Mars". C&EN. http://pubs.acs.org/cen/news/86/i31/8631news6.html.
* Beck, Charles M. II. (February 15, 1994). "Classical Analysis: A Look at the Past, Present, and Future". Anal. Chem. 66 (4): 224A–239A. doi:10.1021/ac00076a718. https://pubs.acs.org/doi/10.1021/ac00076a718.

## References

1. ↑ Trusova, Elena A.; Vokhmintcev, Kirill V.; Zagainov, Igor V. (2012). "Wet-chemistry processing of powdery raw material for high-tech ceramics". Nanoscale Research Letters 7 (1): 11. doi:10.1186/1556-276X-7-58. PMID 22221657. Bibcode: 2012NRL.....7...58T.
2. ↑ Godfrey, Alexander G.; Michael, Samuel G.; Sittampalam, Gurusingham Sitta; Zahoránszky-Köhalmi, Gergely (2020). "A Perspective on Innovating the Chemistry Lab Bench". Frontiers in Robotics and AI 7: 24. doi:10.3389/frobt.2020.00024. ISSN 2296-9144. PMID 33501193.
3. ↑ Dunnivant, F. M.; Elzerman, A. W. (1988). "Determination of polychlorinated biphenyls in sediments, using sonication extraction and capillary column gas chromatography-electron capture detection with internal standard calibration". Journal of the Association of Official Analytical Chemists 71 (3): 551–556. doi:10.1093/jaoac/71.3.551. ISSN 0004-5756. PMID 3134332. https://pubmed.ncbi.nlm.nih.gov/3134332/.
4. ↑ Federherr, E.; Cerli, C.; Kirkels, F. M. S. A.; Kalbitz, K.; Kupka, H. J.; Dunsbach, R.; Lange, L.; Schmidt, T. C. (2014-12-15). "A novel high-temperature combustion based system for stable isotope analysis of dissolved organic carbon in aqueous samples. I: development and validation". Rapid Communications in Mass Spectrometry 28 (23): 2559–2573. doi:10.1002/rcm.7052. ISSN 1097-0231. PMID 25366403. Bibcode: 2014RCMS...28.2559F. https://pubmed.ncbi.nlm.nih.gov/25366403/.
5. ↑ Jackson, P.; Baker, R. J.; McCulloch, D. G.; Mackey, D. W.; van der Wall, H.; Willett, G. D. (June 1996). "A study of Technegas employing X-ray photoelectron spectroscopy, scanning transmission electron microscopy and wet-chemical methods". Nuclear Medicine Communications 17 (6): 504–513. doi:10.1097/00006231-199606000-00009. ISSN 0143-3636. PMID 8822749. https://pubmed.ncbi.nlm.nih.gov/8822749/.
6. ↑ Costantini, Marco; Colosi, Cristina; Święszkowski, Wojciech; Barbetta, Andrea (2018-11-09). "Co-axial wet-spinning in 3D bioprinting: state of the art and future perspective of microfluidic integration". Biofabrication 11 (1): 012001. doi:10.1088/1758-5090/aae605. ISSN 1758-5090. PMID 30284540. https://pubmed.ncbi.nlm.nih.gov/30284540/.
7. ↑ Vagnozzi, Roberto; Signoretti, Stefano; Tavazzi, Barbara; Cimatti, Marco; Amorini, Angela Maria; Donzelli, Sonia; Delfini, Roberto; Lazzarino, Giuseppe (2005). "Hypothesis of the postconcussive vulnerable brain: experimental evidence of its metabolic occurrence". Neurosurgery 57 (1): 164–171; discussion 164–171. doi:10.1227/01.neu.0000163413.90259.85. ISSN 1524-4040. PMID 15987552. https://pubmed.ncbi.nlm.nih.gov/15987552/.
8. ↑ Campbell, A. Malcolm; Zanta, Carolyn A.; Heyer, Laurie J.; Kittinger, Ben; Gabric, Kathleen M.; Adler, Leslie; Schulz, Barbara (2006). "DNA microarray wet lab simulation brings genomics into the high school curriculum". CBE: Life Sciences Education 5 (4): 332–339. doi:10.1187/cbe.06-07-0172. ISSN 1931-7913. PMID 17146040.
9. ↑ Neelamegham, Sriram; Mahal, Lara K. (October 2016). "Multi-level regulation of cellular glycosylation: from genes to transcript to enzyme to structure". Current Opinion in Structural Biology 40: 145–152. doi:10.1016/j.sbi.2016.09.013. ISSN 1879-033X. PMID 27744149.
10. ↑ Makarenko, M. A.; Malinkin, A. D.; Bessonov, V. V.; Sarkisyan, V. A.; Kochetkova, A. A. (2018). "[Secondary lipid oxidation products. Human health risks evaluation (Article 1)"]. Voprosy Pitaniia 87 (6): 125–138. doi:10.24411/0042-8833-2018-10074. ISSN 0042-8833. PMID 30763498. https://pubmed.ncbi.nlm.nih.gov/30763498/.

* v
* t
* e

Analytical chemistry

Instrumentation|

* Atomic absorption spectrometer
* Flame emission spectrometer
* Gas chromatograph
* High-performance liquid chromatograph
* Infrared spectrometer
* Mass spectrometer
* Melting point apparatus
* Microscope
* Optical spectrometer
* Spectrophotometer

Techniques|

* Calorimetry
* Chromatography
* Electroanalytical methods
* Gravimetric analysis
* Ion-mobility spectrometry
* Mass spectrometry
* Spectroscopy
* Titration

Sampling|

* Coning and quartering
* Dilution
* Dissolution
* Filtration
* Masking
* Pulverization
* Sample preparation
* Separation process
* Sub-sampling

Calibration|

* Chemometrics
* Calibration curve
* Matrix effect
* Internal standard
* Standard addition
* Isotope dilution

Prominent publications|

* Analyst
* Analytica Chimica Acta
* Analytical and Bioanalytical Chemistry
* Analytical Chemistry
* Analytical Biochemistry

* Category
* Commons
* Portal
* WikiProject

* v
* t
* e

Branches of chemistry

* Glossary of chemical formulas
* List of biomolecules
* List of inorganic compounds
* Periodic table

Physical|

* Chemical kinetics
* Chemical physics
* Chemical thermodynamics
* Cryochemistry
* Nuclear chemistry
* Electrochemistry
* Femtochemistry
* Geochemistry
* Micromeritics
* Photochemistry
* Quantum chemistry
* Solid-state chemistry
* Spectroscopy
* Spin chemistry
* Surface science
* Thermochemistry

Organic|

* Biochemistry
* Bioorganic chemistry
* Biophysical chemistry
* Chemical biology
* Clinical chemistry
* Fullerene chemistry
* Medicinal chemistry
* Molecular biology
* Neurochemistry
* Physical organic chemistry
* Polymer chemistry

Inorganic|

* Bioinorganic chemistry
* Bioorganometallic chemistry
* Ceramic chemistry
* Cluster chemistry
* Coordination chemistry
* Materials science
* Organometallic chemistry
* Physical inorganic chemistry

Others|

* Actinide chemistry
* Analytical chemistry
* Astrochemistry
* Atmospheric chemistry
* Carbochemistry
* Petrochemistry
* Carbohydrate chemistry
* Chemistry education
* Clay chemistry
* Click chemistry
* Computational chemistry
* Cosmochemistry
* Environmental chemistry
* Food chemistry
* Forensic chemistry
* Green chemistry
* Ocean chemistry
* Post-mortem chemistry
* Sonochemistry
* Stereochemistry
* Supramolecular chemistry
* Theoretical chemistry
* Wet chemistry

See also|

* History of chemistry
* Nobel Prize in Chemistry
* Timeline of chemistry
* Timeline of chemical element discoveries
* The central science
* Chemical reaction
* Chemical element
* Chemical compound
* Atom
* Molecule
* Ion
* Chemical bond

* Category
* Commons
* Portal
* WikiProject

0.00

(0 votes)

Original source: https://en.wikipedia.org/wiki/Wet chemistry. Read more

|

Retrieved from "https://handwiki.org/wiki/index.php?title=Chemistry:Wet_chemistry&oldid=3334823"
*[v]: View this template
*[t]: Discuss this template
*[e]: Edit this template