Shaker (laboratory)

A temperature-controlled shaker, as used in biochemistry work

A shaker is a piece of laboratory equipment used to mix, blend, or agitate substances in a tube or flask by shaking them. It is mainly used in the fields of chemistry and biology. A shaker contains an oscillating board that is used to place the flasks, beakers, or test tubes. Although the magnetic stirrer has lately come to replace the shaker, it is still the preferred choice of equipment when dealing with large volume substances or when simultaneous agitation is required.[1]

## Contents

* 1 Types
* 1.1 Vortex shaker
* 1.2 Platform shaker
* 1.3 Orbital shaker
* 1.4 Incubator shaker
* 2 Images
* 3 See also
* 4 References

## Types

### Vortex shaker

Invented by Jack A. Kraft and Harold D. Kraft in 1962, a vortex shaker is a usually small device used to shake or mix small vials of liquid substance. Its most standout characteristic is that it works by the user putting a vial on the shaking platform and turning it on; thus, the vial is shaken along with the platform. A vortex shaker is very variable in terms of speed adjustment, for the shaking speed can be continuously changed while shaking by turning a switch.[2]

### Platform shaker

A platform shaker has a table board that oscillates horizontally. The liquids to be stirred are held in beakers, jars, or Erlenmeyer flasks that are placed over the table or, sometimes, in test tubes or vials that are nested into holes in the plate.[3] Platform shakers can also be combined with other systems like rotating mixers for small systems and have been designed to be manufactured in laboratories themselves with open source scientific equipment.[4]

### Orbital shaker

An orbital shaker has a circular shaking motion with a slow speed (25-500 rpm). It is suitable for culturing microbes, washing blots, and general mixing. Some of its characteristics are that it does not create vibrations, and it produces low heat compared to other kinds of shakers, which makes it ideal for culturing microbes. Moreover, it can be modified by placing it in an incubator to create an incubator shaker due to its low temperature and vibrations.[5]

### Incubator shaker

An incubator shaker (or thermal shaker) can be considered a mix of an incubator and a shaker. It has an ability to shake while maintaining optimal conditions for incubating microbes or DNA replications. This equipment is very useful since, in order for a cell to grow, it needs oxygen and nutrients that require shaking so that they can be distributed evenly around the culture.[6]

File:Vortex Shaker.webm

Anyone employing an incubator shaker (thermal shaker) to grow yeast or bacteria in the laboratory needs to beware that under the usual conditions encountered in the lab, the rate at which oxygen diffuses from the gaseous phase into the shaken liquid phase is too slow to keep up with the rate at which the oxygen is consumed by, for example, E. coli dividing every half hour or Saccharomyces cerevisiae dividing every hour. If the investigator measure the oxygen in the shake flask on the shaker -- polarographically, for example -- at mid-exponential phase of growth, the dissolved oxygen concentration will turn out to be zero.

## Images

* Thermal shaker for test tubes

* Shaking incubator for culture flasks

* Shaking incubator for culture tubes

* Vortex shaker

## See also

| Wikimedia Commons has media related to laboratory shakers.

|

* Magnetic stirrer
* Vortex mixer
* Stirring rod
* Static mixer

## References

1. ↑ Buie, John. "Evolution of Biological Shakers and Stirrers". http://www.labmanager.com/lab-product/2011/09/evolution-of-biological-shakers-and-stirrers?fw1pk=2#.Vr10wHvRsZO.
2. ↑ Johanna. "What is Vortex mixer and Vortex mixer functions ?". https://neuationcentrifuges.wordpress.com/2014/08/20/what-is-vortex-mixer-and-vortex-mixer-functions/.
3. ↑ "Laboratory Platform Shakers". http://www.biocompare.com/Lab-Equipment/19626-Laboratory-Shakers-Platform/.
4. ↑ Dhankani, Karankumar C.; Pearce, Joshua M. (2017). "Open source laboratory sample rotator mixer and shaker". HardwareX 1: 1–12. doi:10.1016/j.ohx.2016.07.001. https://hal.archives-ouvertes.fr/hal-02113463/file/Open_source_laboratory_sample_rotator_mi.pdf.
5. ↑ BT&C Inc.. "Standard Orbital Shaker". http://btc-bti.com/product_literature/orbitalshakerspecs.htm.
6. ↑ "Laboratory Incubator Shakers". http://www.biocompare.com/Lab-Equipment/19614-Laboratory-Incubator-Shakers-Standard/?search=orbital+shaker.

* v
* t
* e

Laboratory equipment

General

* Heaters
* Dryers

* Alcohol burner
* Bunsen burner
* Desiccator
* Heating mantle
* Hot plate
* Lab oven
* Kiln
* Meker–Fisher burner
* Striker
* Teclu burner
* Water bath
* Vacuum dry box

|

* Mixers
* Shakers

* Chemostat
* Homogenizer
* Liquid whistle
* Magnetic stirrer
* Mortar and pestle
* Shaker
* Sonicator
* Static mixer
* Stirring rod
* Vortex mixer
* Wash bottle

* Stands
* Clamps
* Holders

* Beaker clamp
* Clamp holder
* Tripod
* Burette clamp
* Extension clamp
* Flask clamp
* Funnel support
* Iron ring
* Pinch clamp
* Retort stand
* Screw clamp
* Test tube holder
* Test tube rack
* Wire gauze
* Lab drying rack

* Containers
* Storage

* Agar plate
* Cryogenic storage dewar
* Incubator
* Laminar flow cabinet
* Microtiter plate
* Petri dish
* Picotiter plate
* Refrigerator
* Weighing boat
* Weighing dish

Other items|

* Aspirator
* Autoclave
* Balance brush
* Cork borer
* Crucible
* Filter paper
* File
* Forceps
* Centrifuge
* Microscope
* Pipeclay triangle
* Spectrophotometer
* Splint
* Stopper
* Scoopula
* Spatula
* Test tube brush
* Wire brush
* Inoculation needle
* Inoculation loop

Glassware

| Apparatus|

* Dean–Stark
* Soxhlet extractor
* Kipp's

|

Bottles|

* Boston round
* Pycnometer

Condensers|

* Cold finger
* Liebig

Dishes|

* Evaporating
* Petri
* Syracuse
* Watch glass

Flasks|

* Büchner
* Vacuum (Dewar)
* Erlenmeyer
* Fernbach
* Fleaker
* Florence
* Retort
* Round-bottom
* Schlenk
* Volumetric

Funnels|

* Büchner
* Hirsch
* Dropping
* Separatory

Measuring devices|

* Burette
* Conical measure
* Cuvette
* Eudiometer
* Graduated cylinder
* Ostwald viscometer
* Pipette
* Pipette (dropper)

Tubes|

* Boiling
* Drying
* Cragie
* Ignition
* Nuclear magnetic resonance (NMR)
* Test
* Thiele
* Thistle

Other items|

* Beaker
* Bell jar
* Gas syringe
* Vial

Analytical chemistry

| Compositional|

* AutoAnalyzer
* CHN analyzer
* Colorimeter
* Inductively coupled plasma (ICP) device
* Gas chromatograph (GC)
* Liquid Chromatograph (LC)
* Mass spectrometer (MS)
* pH indicator
* pH meter

|

Microscopy|

* Scanning electron microscope (SEM)
* Transmission electron microscope (TEM)

Thermochemistry|

* Calorimeter
* differential scanning
* Melting-point apparatus
* Thermometer
* Thermogravimetric analyzer (TGA)

Other items|

* Analytical balance
* Colony counter
* Spiral plater
* Nuclear magnetic resonance (NMR) instrument
* Plate reader

Electronics

* Ammeter
* Current source
* Function generator
* Galvanostat
* Multimeter
* Network analyzer
* Oscilloscope
* Pulse generator
* Potentiostat
* Spectrum analyzer
* Time-domain reflectometer
* Voltage source
* Voltmeter

Safety

Personal protective
equipment (PPE)

* Lab coat
* Face shield
* Respirator
* Rubber apron
* Safety shower

Eye and hand|

* Acid-resistant gloves
* Eyewash station
* Glove box
* Medical gloves
* Nitrile gloves
* Safety glasses
* Safety goggles

Other items|

* Acid (solvent) cabinet
* Biosafety cabinet
* Fire blanket
* Fire extinguisher
* Fume hood

Instruments used in medical laboratories

0.00

(0 votes)

Original source: https://en.wikipedia.org/wiki/Shaker (laboratory). Read more

|

Retrieved from "https://handwiki.org/wiki/index.php?title=Engineering:Shaker_(laboratory)&oldid=908407"
*[v]: View this template
*[t]: Discuss this template
*[e]: Edit this template