CORO6

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Identifiers
Aliases|
External IDs| GeneCards: [1]
Orthologs
Species| Human| Mouse
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Ensembl| |

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UniProt| |

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Location (UCSC)| n/a| n/a
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Wikidata
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Coronin-6 also known as coronin-like protein E (Clipin-E) is a protein that in humans is encoded by the CORO6 gene.

Coronin-6 is belongs to coronin family which is an actin binding protein.[1][2] Human CORO6 gene is located on chromosome 17 on the cytogenetic band 17 p11.2.[3] Gene CORO6 is well conserved across domain of eukaryotic organisms from animal to fungi.[4]

## Contents

* 1 Expression
* 1.1 EST profile
* 1.2 Transcript Variant
* 2 Structure
* 3 Homology
* 3.1 Paralogs
* 3.2 Orthologs
* 4 Clinical significance
* 5 Model organisms
* 6 References
* 7 External links

## Expression

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### EST profile

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Based on the EST profile, CORO6 expressed in high level at the larynx, nerve and muscle. CORO6 has also been shown to be expressed in high levels in the breast (mammary gland) tumor. During the human development stage,the higher level of CORO6 expressed at blastocyst and adult.[5]

### Transcript Variant

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Alternative mRNAs are shown aligned from 5' to 3' on a virtual genome where introns have been shrunk to a minimal length. Exon size is proportional to length, intron height reflects the number of cDNAs supporting each intron. Introns of the same color are identical, of different colors are different. 'Good proteins' are pink, partial or not-good proteins are yellow, uORFs are green. 5' cap or3' poly A flags show completeness of the transcript . CORO6 contains 21 distinct gt-ag introns. Transcription produces 10 alternatively spliced mRNA. There are 3 probable alternative promoters, and validated alternative polyadenylation sites.[6]

File:ACEview, predicted alternative splicing sites.png

## Structure

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CORO6 protein sequence contains WD-40 repeats. WD40 domain is a structural motif found in Eukaryotes and cover variety of functions, such as adaptor or regulatory modules in signal transduction, pre-mRNA processing and cytoskeletal assembly. It usually terminating at WD dipeptide at its C-terminus and is about 40 residues long, so called WD40.[7]

The structure of CORO6 is predicted by using Phyre2 program. It is similar to the crystal structure of murine coronin-1. 390 residues ( 83% of CORO6 protein sequence) have been modelled with 100.0% confidence by the single highest scoring template. Image coloured by rainbow N → C terminus

## Homology

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### Paralogs

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Human proteins which are the paralogs to CORO6, CORO1A, CORO1B, CORO1C, CORO2A, CORO2B, CORO7

The table compared Homo sapiens protein CORO6 to its paralogs

Name of paralogs | CORO6 | CORO1A | CORO1B | CORO2A | CORO2B | CORO7

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Accession number | NP_116243 | NP_009005 | NP_065174 | NP_438171 | NP_006082 | NP_078811
Sequence length | 472 aa | 461 aa | 489 aa | 525 aa | 480 aa | 925 aa
Sequence identity |  | 67% | 67% | 45% | 45% | 32%
Sequence similarity |  | 81% | 80% | 64% | 63% | 49%

By comparing its paralogs we found that CORO1A and CORO1B are most related to CORO6.

### Orthologs

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CORO6 is highly conserved throughout the organisms from vertebrate to fungus, the organisms listed in the table are some representatives.

Genus and species (Orthologs comparison) | Homo sapiens | Pan troglodytes | Canis familiaris | Anolis carolinensis | Danio rerio | Saccharomyces cerevisiae | Plasmodium falciparum

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Common name | Human | Chimpanzee | Dog | Lizard | Zebrafish | Baker's Yeast | Malaria parasite
Date of divergence from human lineage |  | 6.3 MYA | 94.2 MYA | 269 MYA | 400.1 MYA | 1215.8 MYA | 1381.2 MYA
Accession number | NP_116243 | XP_001137660 | XP_548302 | XP_0003227217 | NP_956690 | NP_013533 | XP_001350896
Sequence length | 472 aa | 471 aa | 472 aa | 471 aa | 436 aa | 651 aa | 602 aa
Sequence identity to human |  | 96% | 98% | 83% | 78% | 42% | 31%
Sequence similarity to human |  | 97% | 99% | 90% | 90% | 62% | 52%
File:CORO6 nasal epithelium response to house dust mite allergen in vitro.png

## Clinical significance

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There are several clinical studies about that have been performed by using microarray indicating that CORO6 is positively related to allergic nasal epithelium response to house dust mite allergen in vitro.[8]

## Model organisms

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Model organisms have been used in the study of CORO6 function. A conditional knockout mouse line called Coro6tm1e(EUCOMM)Wtsi was generated at the Wellcome Trust Sanger Institute.[9] Male and female animals underwent a standardized phenotypic screen[10] to determine the effects of deletion.[11][12][13][14] Additional screens performed: - In-depth immunological phenotyping[15]

Coro6 knockout mouse phenotype  Characteristic | Phenotype

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All data available at.[10][15]
Peripheral blood leukocytes 6 Weeks | Normal
Insulin | Normal
Haematology 6 Weeks | Normal
Homozygous viability at P14 | Normal
Homozygous Fertility | Normal
Body weight | Normal
Neurological assessment | Normal
Grip strength | Normal
Dysmorphology | Normal
Indirect calorimetry | Normal
Glucose tolerance test | Normal
Auditory brainstem response | Normal
DEXA | Normal
Radiography | Normal
Eye morphology | Normal
Clinical chemistry | Normal
Haematology 16 Weeks | Normal
Peripheral blood leukocytes 16 Weeks | Normal
Heart weight | Normal
Salmonella infection | Normal
Cytotoxic T Cell Function | Normal
Spleen Immunophenotyping | Normal
Mesenteric Lymph Node Immunophenotyping | Normal
Bone Marrow Immunophenotyping | Normal
Epidermal Immune Composition | Normal
Influenza Challenge | Normal

## References

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1. ↑ de Hostos EL, Eugenio L (September 1999). "The coronin family of actin-associated proteins". Trends Cell Biol. 9 (9): 345–50. doi:10.1016/S0962-8924(99)01620-7. PMID 10461187.
2. ↑ "Cronin-6 Homo Sapiens". NCBI. Retrieved 28 April 2013.
3. ↑ "CORO6". GeneCards. Retrieved 28 April 2013.
4. ↑ "CORO6". HomoloGene. Retrieved 28 April 2013.
5. ↑ "CORO6 expression level". EST profile. Retrieved 28 April 2013.
6. ↑ "CORO6". ACEview.
7. ↑ "WD-40 superfamily". conserved domain. Retrieved 28 April 2013.
8. ↑ "Allergic nasal epithelium response to house dust mite allergen in vitro.pnj". GEO profile. Retrieved 9 May 2013.
9. ↑ Gerdin AK (2010). "The Sanger Mouse Genetics Programme: high throughput characterisation of knockout mice". Acta Ophthalmologica. 88: 925–7. doi:10.1111/j.1755-3768.2010.4142.x.
10. ↑ 10.0 10.1 "International Mouse Phenotyping Consortium".
11. ↑ Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu J, Nefedov M, de Jong PJ, Stewart AF, Bradley A (June 2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature. 474 (7351): 337–42. doi:10.1038/nature10163. PMC 3572410. PMID 21677750.
12. ↑ Dolgin E (June 2011). "Mouse library set to be knockout". Nature. 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718.
13. ↑ Collins FS, Rossant J, Wurst W (Jan 2007). "A mouse for all reasons". Cell. 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247.
14. ↑ White JK, Gerdin AK, Karp NA, Ryder E, Buljan M, Bussell JN, Salisbury J, Clare S, Ingham NJ, Podrini C, Houghton R, Estabel J, Bottomley JR, Melvin DG, Sunter D, Adams NC, Sanger Institute Mouse Genetics Project, Tannahill D, Logan DW, Macarthur DG, Flint J, Mahajan VB, Tsang SH, Smyth I, Watt FM, Skarnes WC, Dougan G, Adams DJ, Ramirez-Solis R, Bradley A, Steel KP (2013). "Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes". Cell. 154 (2): 452–64. doi:10.1016/j.cell.2013.06.022. PMC 3717207. PMID 23870131.
15. ↑ 15.0 15.1 "Infection and Immunity Immunophenotyping (3i) Consortium".

## External links

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* Human CORO6 genome location and CORO6 gene details page in the UCSC Genome Browser.