Caffeine

Template:Chembox new

WikiDoc Resources for Caffeine

Articles

Most recent articles on Caffeine

Most cited articles on Caffeine

Review articles on Caffeine

Articles on Caffeine in N Eng J Med, Lancet, BMJ

Media

Powerpoint slides on Caffeine

Images of Caffeine

Photos of Caffeine

Podcasts & MP3s on Caffeine

Videos on Caffeine

Evidence Based Medicine

Cochrane Collaboration on Caffeine

Bandolier on Caffeine

TRIP on Caffeine

Clinical Trials

Ongoing Trials on Caffeine at Clinical Trials.gov

Trial results on Caffeine

Clinical Trials on Caffeine at Google

Guidelines / Policies / Govt

US National Guidelines Clearinghouse on Caffeine

NICE Guidance on Caffeine

NHS PRODIGY Guidance

FDA on Caffeine

CDC on Caffeine

Books

Books on Caffeine

News

Caffeine in the news

Be alerted to news on Caffeine

News trends on Caffeine

Commentary

Blogs on Caffeine

Definitions

Definitions of Caffeine

Patient Resources / Community

Patient resources on Caffeine

Discussion groups on Caffeine

Patient Handouts on Caffeine

Directions to Hospitals Treating Caffeine

Risk calculators and risk factors for Caffeine

Healthcare Provider Resources

Symptoms of Caffeine

Causes & Risk Factors for Caffeine

Diagnostic studies for Caffeine

Treatment of Caffeine

Continuing Medical Education (CME)

CME Programs on Caffeine

International

Caffeine en Espanol

Caffeine en Francais

Business

Caffeine in the Marketplace

Patents on Caffeine

Experimental / Informatics

List of terms related to Caffeine

Editor-In-Chief: C. Michael Gibson, M.S., M.D. [1]; Associate Editor(s)-in-Chief:  Kiran Singh, M.D. [2]

## Overview

[edit | edit source]

Caffeine is a bitter white crystalline xanthine alkaloid that acts as a psychoactive stimulant drug and a mild diuretic (speeds up urine production)[1] in humans and other animals. Caffeine was discovered by a German chemist, Friedrich Ferdinand Runge, in 1819. He coined the term "kaffein", a chemical compound in coffee, which in English became caffeine.[2] Caffeine is also called guaranine when found in guarana, mateine when found in mate, and theine when found in tea; all of these names are synonyms for the same chemical compound.

Caffeine is found in varying quantities in the beans, leaves, and fruit of over 60 plants, where it acts as a natural pesticide that paralyzes and kills certain insects feeding on the plants. It is most commonly consumed by humans in infusions extracted from the beans of the coffee plant and the leaves of the tea bush, as well as from various foods and drinks containing products derived from the kola nut or from cacao. Other sources include yerba mate, guarana berries, and the Yaupon Holly.

In humans, caffeine is a central nervous system (CNS) stimulant, having the effect of temporarily warding off drowsiness and restoring alertness. Beverages containing caffeine, such as coffee, tea, soft drinks and energy drinks enjoy great popularity. Caffeine is the world's most widely consumed psychoactive substance, but unlike most others, it is legal and unregulated in nearly all jurisdictions. In North America, 90% of adults consume caffeine daily.[3] The U.S. Food and Drug Administration lists caffeine as a "Multiple Purpose Generally Recognized as Safe Food Substance".[4] One 2008 study suggested that women consuming 200 milligrams or more of caffeine per day had about twice the miscarriage risk as women who drank none, while another 2008 study found no link between miscarriage and caffeine consumption.[5]

## Occurrence

[edit | edit source]

Roasted coffee beans, the world's primary source of caffeine

Caffeine is a plant alkaloid, found in many plant species, where it acts as a natural pesticide, with high caffeine levels being reported in seedlings that are still developing foliages, but are lacking mechanical protection;[6] caffeine paralyzes and kills certain insects feeding upon the plant.[7] High caffeine levels have also been found in the surrounding soil of coffee bean seedlings. It is therefore understood that caffeine has a natural function as both a natural pesticide and as an inhibitor of seed germination of other nearby coffee seedlings[8] thus giving it a better chance of survival.

The most commonly used caffeine-containing plants are coffee, tea, and to a lesser extent[9] cocoa. Other, less commonly used, sources of caffeine include the yerba mate[10] and guarana plants, which are sometimes used in the preparation of teas and energy drinks. Two of caffeine's alternative names, mateine[11] and guaranine,[12] are derived from the names of these plants. Some yerba mate enthusiasts assert that mateine is a stereoisomer of caffeine, which would make it a different substance altogether.[10] This is not true because caffeine is an achiral molecule, and therefore has no enantiomers; nor does it have other stereoisomers. Many natural sources of caffeine also contain widely varying mixtures of other xanthine alkaloids, including the cardiac stimulants theophylline and theobromine and other substances such as polyphenols which can form insoluble complexes with caffeine.[13]

The world's primary source of caffeine is the coffee bean (the seed of the coffee plant), from which coffee is brewed. Caffeine content in coffee varies widely depending on the type of coffee bean and the method of preparation used;[14] even beans within a given bush can show variations in concentration. In general, one serving of coffee ranges from 40 milligrams, for a single shot (30 milliliters) of arabica-variety espresso, to about 100 milligrams for a cup (120 milliliters) of drip coffee. Generally, dark-roast coffee has less caffeine than lighter roasts because the roasting process reduces the bean's caffeine content.[15][16] Arabica coffee normally contains less caffeine than the robusta variety.[14] Coffee also contains trace amounts of theophylline, but no theobromine.

Tea is another common source of caffeine. Tea usually contains about half as much caffeine per serving as coffee, depending on the strength of the brew. Certain types of tea, such as black and oolong, contain somewhat more caffeine than most other teas. Tea contains small amounts of theobromine and slightly higher levels of theophylline than coffee. Preparation has a significant impact on tea, and color is a very poor indicator of caffeine content.[17] Teas like the pale Japanese green tea gyokuro, for example, contain far more caffeine than much darker teas like lapsang souchong, which has very little.

Caffeine is also a common ingredient of soft drinks such as cola, originally prepared from kola nuts. Soft drinks typically contain about 10 to 50 milligrams of caffeine per serving. By contrast, energy drinks such as Red Bull contain as much as 80 milligrams of caffeine per serving. The caffeine in these drinks either originates from the ingredients used or is an additive derived from the product of decaffeination or from chemical synthesis. Guarana, a prime ingredient of energy drinks, contains large amounts of caffeine with small amounts of theobromine and theophylline in a naturally occurring slow-release excipient.[18]

Chocolate derived from cocoa contains a small amount of caffeine. The weak stimulant effect of chocolate may be due to a combination of theobromine and theophylline as well as caffeine.[19] Chocolate contains too little of these compounds for a reasonable serving to create effects in humans that are on par with coffee. A typical 28-gram serving of a milk chocolate bar has about as much caffeine as a cup of decaffeinated coffee.

In recent years various manufacturers have begun putting caffeine into shower products such as shampoo and soap, claiming that caffeine can be absorbed through the skin.[20] However, the effectiveness of such products has not been proven, and they are likely to have little stimulatory effect on the central nervous system because caffeine is not readily absorbed through the skin.[21]

In recent years various manufacturers have begun putting caffeine into tablets, claiming that using caffeine of pharmaceutical quality improves mental alertness and is used by students that are studying for their exams. It is also used by people who work or drive for long hours. [22]

## History

[edit | edit source]

A coffeehouse in Palestine, circa 1900

Main articles: History of cocoa, History of coffee, Origin and early history of tea

Humans have consumed caffeine since the Stone Age.[23] Early peoples found that chewing the seeds, bark, or leaves of certain plants had the effects of easing fatigue, stimulating awareness, and elevating mood. Only much later was it found that the effect of caffeine was increased by steeping such plants in hot water. Many cultures have legends that attribute the discovery of such plants to people living many thousands of years ago.

According to one popular Chinese legend, the Emperor of China Shennong, reputed to have reigned in about 3000 BC, accidentally discovered that when some leaves fell into boiling water, a fragrant and restorative drink resulted.[24] Shennong is also mentioned in Lu Yu's Cha Jing, a famous early work on the subject of tea.[25] The history of coffee has been recorded as far back as the ninth century. During that time, coffee beans were available only in their native habitat, Ethiopia. A popular legend traces its discovery to a goatherder named Kaldi, who apparently observed goats that became elated and sleepless at night after browsing on coffee shrubs and, upon trying the berries that the goats had been eating, experienced the same vitality. The earliest literary mention of coffee may be a reference to Bunchum in the works of the 9th century Persian physician al-Razi. In 1587, Malaye Jaziri compiled a work tracing the history and legal controversies of coffee, entitled "Undat al safwa fi hill al-qahwa". In this work, Jaziri recorded that one Sheikh, Jamal-al-Din al-Dhabhani, mufti of Aden, was the first to adopt the use of coffee in 1454, and that in the 15th century the Sufis of Yemen routinely used coffee to stay awake during prayers.

Towards the close of the 16th century, the use of coffee was recorded by a European resident in Egypt, and about this time it came into general use in the Near East. The appreciation of coffee as a beverage in Europe, where it was first known as "Arabian wine," dates from the 17th century. During this time "coffee houses" were established, the first being opened in Constantinople and Venice. In Britain, the first coffee houses were opened in London in 1652, at St Michael's Alley, Cornhill. They soon became popular throughout Western Europe, and played a significant role in social relations in the 17th and 18th centuries.[26]

The kola nut, like the coffee berry and tea leaf, appears to have ancient origins. It is chewed in many West African cultures, individually or in a social setting, to restore vitality and ease hunger pangs. In 1911, kola became the focus of one of the earliest documented health scares when the US government seized 40 barrels and 20 kegs of Coca-Cola syrup in Chattanooga, Tennessee, alleging that the caffeine in its drink was "injurious to health".[27] On March 13, 1911, the government initiated The United States v. Forty Barrels and Twenty Kegs of Coca-Cola, hoping to force Coca-Cola to remove caffeine from its formula by making claims, such as that the excessive use of Coca-Cola at one girls' school led to "wild nocturnal freaks, violations of college rules and female proprieties, and even immoralities."[28] Although the judge ruled in favor of Coca-Cola, two bills were introduced to the U.S. House of Representatives in 1912 to amend the Pure Food and Drug Act, adding caffeine to the list of "habit-forming" and "deleterious" substances which must be listed on a product's label.

The earliest evidence of cocoa use comes from residue found in an ancient Mayan pot dated to 600 BC. In the New World, chocolate was consumed in a bitter and spicy drink called xocoatl, often seasoned with vanilla, chile pepper, and achiote. Xocoatl was believed to fight fatigue, a belief that is probably attributable to the theobromine and caffeine content. Chocolate was an important luxury good throughout pre-Columbian Mesoamerica, and cocoa beans were often used as currency.

Chocolate was introduced to Europe by the Spaniards and became a popular beverage by 1700. They also introduced the cacao tree into the West Indies and the Philippines. It was used in alchemical processes, where it was known as Black Bean.

In 1819, the German chemist Friedrich Ferdinand Runge isolated relatively pure caffeine for the first time. According to Runge, he did this at the behest of Johann Wolfgang von Goethe.[29] In 1827, Oudry isolated "theine" from tea, but it was later proved by Mulder and Jobat that theine was the same as caffeine.[29] The structure of caffeine was elucidated near the end of the 19th century by Hermann Emil Fischer, who was also the first to achieve its total synthesis.[30] This was part of the work for which Fischer was awarded the Nobel Prize in 1902.

Today, global consumption of caffeine has been estimated at 120,000 tonnes per annum,[31] making it the world's most popular psychoactive substance. This number equates to one serving of a caffeine beverage for every person, per day. In North America, 90% of adults consume some amount of caffeine daily.[3]

## Pharmacology

[edit | edit source]

Caffeine is a central nervous system and metabolic stimulant,[32] and is used both recreationally and medically to reduce physical fatigue and restore mental alertness when unusual weakness or drowsiness occurs. Caffeine stimulates the central nervous system first at the higher levels, resulting in increased alertness and wakefulness, faster and clearer flow of thought, increased focus, and better general body coordination, and later at the spinal cord level at higher doses.[33] Once inside the body, it has a complex chemistry, and acts through several mechanisms as described below.

### Metabolism

[edit | edit source]

Caffeine is metabolized in the liver into three primary metabolites: paraxanthine (84%), theobromine (12%), and theophylline (4%)

Caffeine is completely absorbed by the stomach and small intestine within 45 minutes of ingestion. After ingestion it is distributed throughout all tissues of the body and is eliminated by first-order kinetics.[34]

The half-life of caffeine—the time required for the body to eliminate one-half of the total amount of caffeine consumed at a given time—varies widely among individuals according to such factors as age, liver function, pregnancy, some concurrent medications, and the level of enzymes in the liver needed for caffeine metabolism. In healthy adults, caffeine's half-life is approximately 3-4 hours. In women taking oral contraceptives this is increased to 5-10 hours,[35] and in pregnant women the half-life is roughly 9-11 hours.[36] Caffeine can accumulate in individuals with severe liver disease when its half-life can increase to 96 hours.[37] In infants and young children, the half-life may be longer than in adults; half-life in a newborn baby may be as long as 30 hours. Other factors such as smoking can shorten caffeine's half-life.[38]

Caffeine is metabolized in the liver by the cytochrome P450 oxidase enzyme system (specifically, the 1A2 isozyme) into three metabolic dimethylxanthines,[39] which each have their own effects on the body:

* Paraxanthine (84%): Has the effect of increasing lipolysis, leading to elevated glycerol and free fatty acid levels in the blood plasma.
* Theobromine (12%): Dilates blood vessels and increases urine volume. Theobromine is also the principal alkaloid in cocoa, and therefore chocolate.
* Theophylline (4%): Relaxes smooth muscles of the bronchi, and is used to treat asthma. The therapeutic dose of theophylline, however, is many times greater than the levels attained from caffeine metabolism.

Each of these metabolites is further metabolized and then excreted in the urine.

### Mechanism of action

[edit | edit source]

Caffeine's principal mode of action is as an antagonist of adenosine receptors in the brain. They are presented here side by side for comparison.

Caffeine acts through multiple mechanisms involving both action on receptors and channels on the cell membrane, as well as intracellular action on calcium and cAMP pathways. By virtue of its purine structure it can act on some of the same targets as adenosine related nucleosides and nucleotides, like the cell surface P1 GPCRs for adenosine, as well as the intracellular Ryanodine receptor (RyR) which is the physiological target of cADPR (cyclic ADP-ribose), and cAMP-phosphodiesterase (cAMP-PDE). Although the action is agonistic in some cases, it is antagonistic in others. Physiologically, however, caffeine action is unlikely due to increased RyR opening, as it requires plasma concentration above lethal dosage. The action is most likely through adenosine receptors.[citation needed]

Like alcohol, nicotine, and antidepressants, caffeine readily crosses the blood brain barrier. Once in the brain, the principal mode of action of caffeine is as an antagonist of adenosine receptors found in the brain.[40] The caffeine molecule is structurally similar to adenosine, and binds to adenosine receptors on the surface of cells without activating them (an "antagonist" mechanism of action). Therefore, caffeine acts as a competitive inhibitor.

Caffeine being a competitive inhibitor of adenosine, an understanding of adenosine’s role in the central nervous system is crucial. One of the roles of adenosine is as a signal that one neuron can use to tell another to stop releasing neurotransmitter because it can’t handle the stimulation. In doing this it is acting as a retrograde neurotransmitter (a neurotransmitter that is released by the post-synaptic cell and received by the pre-synaptic cell in the direction opposite to most neurotransmitters).[41] Adenosine is the final breakdown product of adenosine triphosphate (ATP), which is the cellular currency of energy. When cells have used the energy of adenosine triphosphate it breaks into adenosine diphosphate, which is then used for energy and broken down into adenosine monophosphate. Finally, the last phosphate bond is broken for energy in the cell’s last attempt to squeeze molecular power from this molecule: adenosine monophosphate is broken down into simple adenosine. At this point, the neuron has very little energy left for the successful firing of an action potential. Adenosine from this process is then released from the postsynaptic cell and binds to receptors on the presynaptic cell. If the release of adenosine is great enough, this release has an inhibitory effect on the release of neurotransmitter from the presynaptic neuron’s axon terminal. This triggers a mechanism that inhibits the further secretion of excitatory neurotransmitters into the synapse. It is as if the postsynaptic neuron is telling the presynaptic neuron that its resources are scarce and it needs time to recover before further stimulation by neurotransmitters. Thus, adenosine works to inhibit activity of the central nervous system.

Caffeine being a competitive inhibitor of adenosine, it binds to the adenosine receptor, but does not trigger the chemical cascade that inhibits neurotransmitter release and blocks the site so adenosine cannot bind and get its message across the synapse. By inhibiting adenosine, caffeine excites the central nervous system and allows for continued stimulation of neurons that otherwise would not fire or would not release neurotransmitter into the synapse.[41][42][43][44] The reduction in adenosine activity results in increased activity of the neurotransmitter dopamine, largely accounting for its stimulatory effects. This inhibition of adenosine is the only known biochemical effect that caffeine has in humans at the concentrations achieved during normal human consumption of the drug.[41] Further, coffee & tea drinkers on the whole do not consume enough caffeine to release dopamine in the nucleus accumbens shell, the key structure associated with motivation, reward & addiction. However, in high doses, caffeine induces dopamine release in nucleus accumbens, not unlike other psychostimulants, such as cocaine.[45] In low and moderate doses, caffeine appears to increase dopamine and acetylcholine release in the prefrontal cortex (PFC) (providing a mechanism for reinforcment) and stimulates the caudate nucleus, increasing wakefulness & locomotor activity. Sustained caffeine usage causes tolerance to the (prefrontal cortical) dopamine-mediated effects and hence the locomotor stimulation, but not to its cholinergic effects in the PFC, which could account for its capability for sustained arousal even in the caffeine-tolerant.[46][47][48] Caffeine also increases levels of epinephrine/adrenaline,[49] possibly via a different mechanism. Acute usage of caffeine also increases levels of serotonin, causing positive changes in mood.[50]

The inhibition of adenosine may be relevant in its diuretic properties. Because adenosine is known to constrict preferentially the afferent arterioles of the glomerulus, limiting blood flow and therefore urine production; its inhibition may cause vasodilation, with an increase in renal blood flow (RBF) and glomerular filtration rate (GFR). This effect, called competitive inhibition, interrupts a pathway that normally serves to regulate nerve conduction by suppressing post-synaptic potentials. The result is an increase in the levels of epinephrine and norepinephrine/noradrenaline released via the hypothalamic-pituitary-adrenal axis.[50] Epinephrine, the natural endocrine response to a perceived threat, stimulates the sympathetic nervous system, leading to an increased heart rate, blood pressure and blood flow to muscles, a decreased blood flow to the skin and inner organs. Biochemically, it stimulates glycogenolysis, inhibits glycolysis, and stimulates gluconeogenesis to produce more glucose in the muscles and release of glucose into the blood stream from the liver.

Caffeine is also a known competitive inhibitor of the enzyme cAMP-phosphodiesterase (cAMP-PDE), which converts cyclic AMP (cAMP) in cells to its noncyclic form, allowing cAMP to build up in cells. Cyclic AMP participates in activation of Protein Kinase A (PKA) to begin the phosphorylation of specific enzymes used in glucose synthesis. By blocking its removal caffeine intensifies and prolongs the effects of epinephrine and epinephrine-like drugs such as amphetamine, methamphetamine, or methylphenidate. Increased concentrations of cAMP in parietal cells causes an increased activation of protein kinase A (PKA) which in turn increases activation of H+/K+ ATPase, resulting finally in increased gastric acid secretion by the cell.

Caffeine (and theophylline) can freely diffuse into cells and causes intracellular calcium release (independent of extracellular calcium) from the calcium stores in the endoplasmic reticulum(ER). This release is only partially blocked by Ryanodine receptor blockade with ryanodine, dantrolene, ruthenium red, and procaine (thus may involve ryanodine receptor and probably some additional calcium channels), but completely abolished after calcium depletion of ER by SERCA inhibitors like Thapsigargin (TG) or cyclopiazonic acid (CPA).[51] The action of caffeine on the ryanodine receptor may depend on both cytosolic and the luminal ER concentrations of Ca2+. At low millimolar concentration of caffeine, the RyR channel open probability (Po) is significantly increased mostly due to a shortening of the lifetime of the closed state. At concentrations >5 mM, caffeine opens RyRs even at picomolar cytosolic Ca2+ and dramatically increases the open time of the channel so that the calcium release is stronger than even an action potential can generate. This mode of action of caffeine is probably due to mimicking the action of the physiologic metabolite of NAD called cADPR (cyclic ADP ribose) which has a similar potentiating action on Ryanodine receptors.

Caffeine may also directly inhibit delayed rectifier and A-type K+ currents and activate plasmalemmal Ca2+ influx in certain vertebrate and invertebrate neurons.

The metabolites of caffeine contribute to caffeine's effects. Theobromine is a vasodilator that increases the amount of oxygen and nutrient flow to the brain and muscles. Theophylline, the second of the three primary metabolites, acts as a smooth muscle relaxant that chiefly affects bronchioles and acts as a chronotrope and inotrope that increases heart rate and efficiency. The third metabolic derivative, paraxanthine, is responsible for an increase in the lipolysis process, which releases glycerol and fatty acids into the blood to be used as a source of fuel by the muscles.[52]

### Effects when taken in moderation

[edit | edit source]

The precise amount of caffeine necessary to produce effects varies from person to person depending on body size and degree of tolerance to caffeine. It takes less than an hour for caffeine to begin affecting the body and a mild dose wears off in three to four hours.[33] Consumption of caffeine does not eliminate the need for sleep: it only temporarily reduces the sensation of being tired.

With these effects, caffeine is an ergogenic: increasing the capacity for mental or physical labor. A study conducted in 1979 showed a 7% increase in distance cycled over a period of two hours in subjects who consumed caffeine compared to control tests.[53] Other studies attained much more dramatic results; one particular study of trained runners showed a 44% increase in "race-pace" endurance, as well as a 51% increase in cycling endurance, after a dosage of 9 milligrams of caffeine per kilogram of body weight.[54] The extensive boost shown in the runners is not an isolated case; additional studies have reported similar effects. Another study found 5.5 milligrams of caffeine per kilogram of body mass resulted in subjects cycling 29% longer during high intensity circuits.[55]

Caffeine citrate has proven to be of short and long term benefit in treating the breathing disorders of apnea of prematurity and bronchopulmonary displasia in premature infants. Citrated caffeine,[56] the only short term risk associated with caffeine citrate treatment is a temporary reduction in weight gain during the therapy,[57] and longer term studies (18 to 21 months) have shown lasting benefits of treatment of premature infants with caffeine.[58][59]

While relatively safe for humans, caffeine is considerably more toxic to some other animals such as dogs, horses and parrots due to a much poorer ability to metabolize this compound. Caffeine has a much more significant effect on spiders, for example, than most other drugs do.[60]

Caffeine relaxes the internal anal sphincter muscles and thus should be avoided by those with fecal incontinence.[61]

## Intoxication

[edit | edit source]

An acute overdose of caffeine, usually in excess of about 300 milligrams, dependent on body weight and level of caffeine tolerance, can result in a state of central nervous system over-stimulation called caffeine intoxication,[62] colloquially "caffeine jitters". The symptoms of caffeine intoxication are not unlike overdoses of other stimulants. It may include restlessness, nervousness, excitement, insomnia, flushing of the face, increased urination, gastrointestinal disturbance, muscle twitching, a rambling flow of thought and speech, irritability, irregular or rapid heart beat, and psychomotor agitation.[63] In cases of much larger overdoses mania, depression, lapses in judgment, disorientation, loss of social inhibition, delusions, hallucinations, psychosis, rhabdomyolysis, and death may occur.[64][65]

In cases of extreme overdose, death can result. The median lethal dose (LD50) given orally, is 192 milligrams per kilogram in rats.[66] The LD50 of caffeine in humans is dependent on weight and individual sensitivity and estimated to be about 150 to 200 milligrams per kilogram of body mass, roughly 80 to 100 cups of coffee for an average adult taken within a limited time frame that is dependent on half-life. Though achieving lethal dose with caffeine would be exceptionally difficult with regular coffee, there have been reported deaths from overdosing on caffeine pills, with serious symptoms of overdose requiring hospitalization occurring from as little as 2 grams of caffeine.[67][68][69][70] Death typically occurs due to ventricular fibrillation brought about by effects of caffeine on the cardiovascular system.

Treatment of severe caffeine intoxication is generally supportive, providing treatment of the immediate symptoms, but if the patient has very high serum levels of caffeine then peritoneal dialysis, hemodialysis, or hemofiltration may be required.

### DSM-V Diagnostic Criteria for Caffeine Intoxication[71]

[edit | edit source]

“  |

* A. Recent consumption of caffeine (typically a high dose well in excess of 250 mg).

AND

* B. Five (or more) of the following signs or symptoms developing during, or shortly after,caffeine use:

* 1.Restlessness.
* 2.Nervousness.
* 3.Excitement.
* 4.Insomnia.
* 5.Flushed face.
* 6.Diuresis.
* 7.Gastrointestinal disturbance.
* 8.Muscle twitching.
* 9.Rambling flow of thought and speech.
* 10.Tachycardia or cardiac arrhythmia.
* 11.Periods of in exhaustibility.
* 12.Psychomotor agitation.

AND

* C.The signs or symptoms in Criterion B cause clinically significant distress or impairment in social, occupational, or other important areas of functioning.

AND

* D.The signs or symptoms are not attributable to another medical condition and are not better explained by another mental disorder, including intoxication with another substance.

| ”

|
|

### Risk Factors of Caffeine Intoxication

[edit | edit source]

* Recent increase in caffeine intake
* Oral contraceptives
* Use of caffeine less frequently

### Differential Diagnosis of caffeine Intoxication

[edit | edit source]

* Other mental disorders

* Amphetamine intoxication
* Generalized anxiety disorder
* Manic episodes
* Medication-induced side effects
* Panic disorder
* Sedative, hynotic, or anxiolytic withdrawal
* Sleep disorders
* Tobacco withdrawal[71]

* Other caffeine-induced disorders

* Caffeine-induced anxiety disorder
* caffeine-induced sleep disorder[71]

## Tolerance and Withdrawal

[edit | edit source]

Caffeine content of select common food and drugs.[72][73]
Product  | Serving size  | Caffeine per serving (mg)  | Caffeine per litre (mg)

|
|
|

Caffeine tablet (regular strength)  | 1 tablet  | 100  | —
Caffeine tablet (extra strength)  | 1 tablet  | 200  | —
Excedrin tablet  | 1 tablet  | 65  | —
Chocolate, Dark (Hershey's Special Dark)  | 1 bar (43 g; 1.5 oz) | 31  | —
Chocolate, Milk (Hershey Bar)  | 1 bar (43 g; 1.5 oz) | 10  | —
Coffee, brewed  | 207 mL (7 U.S. fl oz) | 80–135  | 386–652
Coffee, drip  | 207 mL (7 U.S. fl oz) | 115–175  | 555–845
Coffee, decaffeinated | 207 mL (7 U.S. fl oz) | 5  | 24
Coffee, espresso | 44–60 mL (1.5-2 U.S. fl oz) | 100  | 1691–2254
Coffee, Starbucks | (Tall 12 U.S. fl oz) | 240  |
Tea, leaf or bag  | 177 mL (6 U.S. fl oz) | 50  | 281
Tea, green  | 177 mL (6 U.S. fl oz) | 30  | 169
Soft drink, Coca-Cola Classic  | 355 mL (12 U.S. fl oz) | 34  | 96
Soft drink, Mountain Dew | 355 mL (12 U.S. fl oz) | 54.5  | 154
Soft drink, Jolt Cola | 694 mL (23.5 U.S. fl oz) | 150  | 216
Red Bull | 250 mL (8.2 U.S. fl oz) | 80  | 320
Monster Energy | 473 mL (16 U.S. fl oz) | 160  | 338
Wired X344 | 473 mL (16 U.S. fl oz) | 344  | 727
Foosh Energy Mints | 1 mint  | 100  | —
Buzz Bites | 1 chew  | 100  | —
Buckfast Tonic Wine | 750 mL (25.4 U.S. fl oz) | 281  | 375

Because caffeine is primarily an antagonist of the central nervous system's receptors for the neurotransmitter adenosine, the bodies of individuals who regularly consume caffeine adapt to the continual presence of the drug by substantially increasing the number of adenosine receptors in the central nervous system. This increase in the number of the adenosine receptors makes the body much more sensitive to adenosine, with two primary consequences.[74] First, the stimulatory effects of caffeine are substantially reduced, a phenomenon known as a tolerance adaptation. Second, because these adaptive responses to caffeine make individuals much more sensitive to adenosine, a reduction in caffeine intake will effectively increase the normal physiological effects of adenosine, resulting in unwelcome withdrawal symptoms in tolerant users.[74]

Other research questions the idea that up-regulation of adenosine receptors is responsible for tolerance to the locomotor stimulant effects of caffeine, noting, among other things, that this tolerance is insurmountable by higher doses of caffeine (it should be surmountable if tolerance was due to an increase in receptors), and that the increase in adenosine receptor number is modest and does not explain the large tolerance which develops to caffeine.[75]

Caffeine tolerance develops very quickly, especially among heavy coffee and energy drink consumers. Complete tolerance to sleep disruption effects of caffeine develops after consuming 400 mg of caffeine 3 times a day for 7 days. Complete tolerance to subjective effects of caffeine was observed to develop after consuming 300 mg 3 times per day for 18 days, and possibly even earlier.[76] In another experiment, complete tolerance of caffeine was observed when the subject consumed 750–1200 mg per day while incomplete tolerance to caffeine has been observed in those that consume more average doses of caffeine.[77]

Because adenosine, in part, serves to regulate blood pressure by causing vasodilation, the increased effects of adenosine due to caffeine withdrawal cause the blood vessels of the head to dilate, leading to an excess of blood in the head and causing a headache and nausea. Reduced catecholamine activity may cause feelings of fatigue and drowsiness. A reduction in serotonin levels when caffeine use is stopped can cause anxiety, irritability, inability to concentrate and diminished motivation to initiate or to complete daily tasks; in extreme cases it may cause mild depression. Together, these effects have come to be known as a "crash".[78]

Withdrawal symptoms—possibly including headache, irritability, an inability to concentrate, and stomach aches[79]—may appear within 12 to 24 hours after discontinuation of caffeine intake, peak at roughly 48 hours, and usually last from one to five days, representing the time required for the number of adenosine receptors in the brain to revert to "normal" levels, uninfluenced by caffeine consumption. Analgesics, such as aspirin, can relieve the pain symptoms, as can a small dose of caffeine.[80] Most effective is a combination of both an analgesic and a small amount of caffeine.

This is not the only case where caffeine increases the effectiveness of a drug. Caffeine makes pain relievers 40% more effective in relieving headaches and helps the body absorb headache medications more quickly, bringing faster relief.[81] For this reason, many over-the-counter headache drugs include caffeine in their formula. It is also used with ergotamine in the treatment of migraine and cluster headaches as well as to overcome the drowsiness caused by antihistamines.

### DSM-V Diagnostic Criteria for Caffeine Withdrawl[71]

[edit | edit source]

“  |

* A. Prolonged daily use of caffeine.

AND

* B. Abrupt cessation of or reduction in caffeine use, followed within 24 hours by three (or more) of the following signs or symptoms:

* 1\. Headache.
* 2\. Marked fatigue or drowsiness.
* 3\. Dysphoric mood, depressed mood, or irritability.
* 4\. Difficulty concentrating.
* 5\. Flu-like symptoms (nausea, vomiting, or muscle pain/stiffness).

AND

* C. The signs or symptoms in Criterion B cause clinically significant distress or impairment in social, occupational, or other important areas of functioning.

AND

* D. The signs or symptoms are not associated with the physiological effects of another medical condition (e.g., migraine, viral illness) and are not better explained by another

mental disorder, including intoxication or withdrawal from another substance.

| ”

|
|

### Epidemiology and Demographics of Caffeine Withdrawal

[edit | edit source]

The prevalence of caffeine withdrawal is unclear in the overall population.[71]

### Risk Factors of Caffeine Withdrawal

[edit | edit source]

* Alcohol abuser
* Drug abuser
* Doses of caffeine significantly less than one's usual daily dose
* Eating disorders
* Genetic predisposition
* Mental disorders
* Prisoners
* Smokers
* Unavailability of caffeineo[71]

### Differential Diagnosis of Caffeine Withdrawal

[edit | edit source]

* Other medical disorders and medical side effects

* Drug withdrawal states
* Headache disorders
* Medication side effects
* Migraine
* Sinus conditions
* Viral illnesseso[71]

## Other Notable Effects of Caffeine

[edit | edit source]

#### Anxiety and sleep disorders

[edit | edit source]

Two infrequently diagnosed caffeine-induced disorders that are recognized by the American Psychiatric Association (APA) are caffeine-induced sleep disorder and caffeine-induced anxiety disorder, which can result from long-term excessive caffeine intake.

In the case of caffeine-induced sleep disorder, an individual regularly ingests high doses of caffeine sufficient to induce a significant disturbance in his or her sleep, sufficiently severe to warrant clinical attention.Closing `</ref>` missing for `<ref>` tag A study in the British Journal of Addiction concluded that caffeinism, although infrequently diagnosed, may afflict as many as one person in ten of the population.[82]

### Parkinson's disease

[edit | edit source]

Several large studies have shown that caffeine intake is associated with a reduced risk of developing Parkinson's disease (PD) in men, but studies in women have been inconclusive.[83] The mechanism by which caffeine affects PD remains a mystery. In animal models, researchers have shown that caffeine can prevent the loss of dopamine-producing nerve cells seen in Parkinson's Disease, but researchers still do not know how this occurs.[84]

### Effects on memory and learning

[edit | edit source]

An array of studies found that caffeine could have nootropic effects, inducing certain changes in memory and learning. However, it is still not definitely clear whether the effect is negative or positive.

Researchers have found that long-term consumption of low dose caffeine slowed hippocampus-dependent learning and impaired long-term memory. Caffeine consumption for 4 weeks also significantly reduced hippocampal neurogenesis compared to controls during the experiment. The conclusion was that long-term consumption of caffeine could inhibit hippocampus-dependent learning and memory partially through inhibition of hippocampal neurogenesis. [85]

In one study, caffeine was added to rat neurons in vitro. The dendritic spines (a part of the brain cell used in forming connections between neurons) taken from the hippocampus (a part of the brain associated with memory) grew by 33% and new spines formed. After an hour or two, however, these cells returned to their original shape.[86]

Another study showed that subjects--after receiving 100 milligrams of caffeine--had increased activity in brain regions located in the frontal lobe, where a part of the working memory network is located, and the anterior cingulum, a part of the brain that controls attention. The caffeinated subjects also performed better on the memory tasks.[87]

However, a different study showed that caffeine could impair short term memory and increase the likelihood of the tip of the tongue phenomenon. The study allowed the researchers to suggest that caffeine could aid short-term memory when the information to be recalled is related to the current train of thought, but also to hypothesize that caffeine hinders short-term memory when the train of thought is unrelated.[88] In essence, focused thought coupled with caffeine consumption increases mental performance.

### Effects on the heart

[edit | edit source]

Caffeine increases the levels of cAMP in the heart cells, mimicking the effects of epinephrine. cAMP diffuses through the cell and acts as a "secondary messenger," activating protein kinase A (PKA; cAMP-dependent protein kinase). According to one study, caffeine, in the form of coffee, significantly reduces the risk of heart disease in epidemiological studies. However, the protective effect was found only in participants who were not severely hypertensive (i.e. patients that are not suffering from a very high blood pressure). Furthermore, no significant protective effect was found in participants aged less than 65 years or in cerebrovascular disease mortality for those aged equal or more than 65 years.[89]

### Effects on children

[edit | edit source]

Scientific studies contradict the common belief that caffeine consumption causes stunted growth in children.[90] However, as with adults, nausea, urinary urgency, nervousness, or other effects from an elevated caffeine intake via chocolate milk, sodas, cold medicines, iced tea, coffee and other products that are widely used, may be reasons to limit the amount of caffeine that is consumed each day.[91]

### Caffeine intake during pregnancy

[edit | edit source]

The Food Standards Agency has recommended that pregnant women should limit their caffeine intake to less than 300 mg of caffeine a day – the equivalent of four cups of coffee a day. A higher intake may be associated with miscarriage.[92][93]

Dr De-Kun Li of Kaiser Permanente Division of Research, which appears in the American Journal of Obstetrics and Gynecology, concludes that an intake of 200 milligrams or more per day, representing two or more cups, "significantly increases the risk of miscarriage".[94] However, Dr. David Savitz, a professor in community and preventive medicine at New York's Mount Sinai School of Medicine and lead author of the other new study on the subject published in the January issue of Epidemiology, found no link between miscarriage and caffeine consumption.[5]

## Production

[edit | edit source]

Anhydrous (dry) USP-grade caffeine

Caffeine extracted from coffee and tea during the decaffeination process is sold or used as an additive. Being readily available as a byproduct of decaffeination, caffeine is not usually synthesized.[95] If desired, it may be synthesized from dimethyl urea and malonic acid.[96]

## Decaffeination

[edit | edit source]

Main article: Decaffeination

Pure caffeine is a white powder, and can be extracted from a variety of natural sources. Caffeine extraction is an important industrial process and can be performed using a number of different solvents. Benzene, chloroform, trichloroethylene and dichloromethane have all been used over the years but for reasons of safety, environmental impact, cost and flavor, they have been superseded by the following main methods:

### Water extraction

[edit | edit source]

Coffee beans are soaked in water. The water, which contains not only caffeine but also many other compounds which contribute to the flavor of coffee, is then passed through activated charcoal, which removes the caffeine. The water can then be put back with the beans and evaporated dry, leaving decaffeinated coffee with a good flavor.[97] Coffee manufacturers recover the caffeine and resell it for use in soft drinks and over-the-counter caffeine tablets.

### Supercritical carbon dioxide extraction

[edit | edit source]

Supercritical carbon dioxide is an excellent nonpolar solvent for caffeine (as well as many other organic compounds), and is safer than the organic solvents that are used for caffeine extraction. The extraction process is simple: CO2 is forced through the green coffee beans at temperatures above 31.1 °C and pressures above 73 atm. Under these conditions, CO2 is in a "supercritical" state: it has gaslike properties which allow it to penetrate deep into the beans but also liquid-like properties which dissolve 97–99% of the caffeine. The caffeine-laden CO2 is then sprayed with high pressure water to remove the caffeine. The caffeine can then be isolated by charcoal adsorption (as above) or by distillation, recrystallization, or reverse osmosis.[97]

### Extraction by nonhazardous organic solvents

[edit | edit source]

Organic solvents such as ethyl acetate present much less health and environmental hazard than previously used chlorinated and aromatic solvents. The hydrolysis products of ethyl acetate are ethanol and acetic acid, both nonhazardous in small quantities. Another method is to use triglyceride oils obtained from spent coffee grounds.

## Stereochemistry

[edit | edit source]

The nitrogen atoms are all essentially planar (in sp2 orbital hybridisation). Even though some are often drawn with three single bonds, the lone pairs on these atoms are involved in resonance with adjacent double-bonded carbon atoms, resulting in the caffeine molecule having aromatic character.

## Religion

[edit | edit source]

Some Mormons and Christian Scientists[98] do not consume caffeine. Followers of both religions believe that God wishes them to be free of all addictions.

The Church of Jesus Christ of Latter-day Saints has said the following in regards to caffeinated beverages, “With reference to cola drinks, the Church has never officially taken a position on this matter, but the leaders of the Church have advised, and we do now specifically advise, against the use of any drink containing harmful habit-forming drugs under circumstances that would result in acquiring the habit. Any beverage that contains ingredients harmful to the body should be avoided.” (Priesthood Bulletin, Feb. 1972, p. 4.) See also Word of Wisdom.

Gaudiya Vaishnava Hindus generally also abstain from caffeine, as it clouds the mind and over-stimulates the senses. To be initiated under a guru, one must have had no caffeine (along with alcohol, nicotine and other drugs) for at least a year.

## Pill Images

[edit | edit source]

Drug Name: Ascomp with Codeine
Ingredient(s): Butalbital[Butalbital], Aspirin[Aspirin], Caffeine[Caffeine], Codeine Phosphate[Codeine]
Imprint: B074
Dosage: 40 mg
Color(s): Blue, Yellow
Shape: Capsule
Size (mm): 22
Score: 1
NDC:519910074

Drug Label Author: Breckenridge Pharmaceutical, Inc.

This pill image is provided by the National Library of Medicine's PillBox.
File:Butalbital Acetaminophen Caffeine and Codeine Phosphate NDC 05913220.jpg

Drug Name: Butalbital, Acetaminophen, Caffeine, and Codeine Phosphate
Ingredient(s): CODEINE PHOSPHATE[CODEINE ANHYDROUS], BUTALBITAL[BUTALBITAL], CAFFEINE[CAFFEINE], ACETAMINOPHEN[ACETAMINOPHEN]
Imprint: WATSON;3220
Dosage: 40 mg
Color(s): Blue, White
Shape: Capsule
Size (mm): 22
Score: 1
NDC:05913220

Drug Label Author: Watson Laboratories, Inc.

This pill image is provided by the National Library of Medicine's PillBox.
File:Butalbital Acetaminophen Caffeine and Codeine Phosphate NDC 06032553.jpg

Drug Name: Butalbital, Acetaminophen, Caffeine, and Codeine Phosphate
Ingredient(s): BUTALBITAL[BUTALBITAL], ACETAMINOPHEN[ACETAMINOPHEN], CAFFEINE[CAFFEINE], CODEINE PHOSPHATE[CODEINE ANHYDROUS]
Imprint: V;3109
Dosage: 40 mg
Color(s): Blue, Grey
Shape: Capsule
Size (mm): 22
Score: 1
NDC:06032553

Drug Label Author: Qualitest Pharmaceuticals

This pill image is provided by the National Library of Medicine's PillBox.
File:Butalbital Acetaminophen and Caffeine NDC 01431787.jpg

Drug Name: Butalbital, Acetaminophen and Caffeine
Ingredient(s): BUTALBITAL[BUTALBITAL], ACETAMINOPHEN[ACETAMINOPHEN], CAFFEINE[CAFFEINE]
Imprint: West;ward;787
Dosage: 40 mg
Color(s): Blue
Shape: Round
Size (mm): 11
Score: 1
NDC:01431787

Drug Label Author: West-Ward Pharmaceutical Corp

This pill image is provided by the National Library of Medicine's PillBox.
File:Butalbital Acetaminophen and Caffeine NDC 05913369.jpg

Drug Name: Butalbital, Acetaminophen, and Caffeine
Ingredient(s): BUTALBITAL[BUTALBITAL], ACETAMINOPHEN[ACETAMINOPHEN], CAFFEINE[CAFFEINE]
Imprint: WATSON;3369
Dosage: 40 mg
Color(s): White
Shape: Round
Size (mm): 11
Score: 1
NDC:05913369

Drug Label Author: Watson Laboratories, Inc.

This pill image is provided by the National Library of Medicine's PillBox.
File:Butalbital Acetaminophen and Caffeine NDC 06032544.jpg

Drug Name: Butalbital, Acetaminophen and Caffeine
Ingredient(s): BUTALBITAL[BUTALBITAL], ACETAMINOPHEN[ACETAMINOPHEN], CAFFEINE[CAFFEINE]
Imprint: 2355;V
Dosage: 40 mg
Color(s): White
Shape: Round
Size (mm): 11
Score: 1
NDC:06032544

Drug Label Author: Qualitest Pharmaceuticals

This pill image is provided by the National Library of Medicine's PillBox.
File:Butalbital Acetaminophen and Caffeine NDC 06032545.jpg

Drug Name: Butalbital, Acetaminophen and Caffeine
Ingredient(s): BUTALBITAL[BUTALBITAL], ACETAMINOPHEN[ACETAMINOPHEN], CAFFEINE[CAFFEINE]
Imprint: 23;57;V
Dosage: 40 mg
Color(s): White
Shape: Oval
Size (mm): 20
Score: 2
NDC:06032545

Drug Label Author: Qualitest Pharmaceuticals

This pill image is provided by the National Library of Medicine's PillBox.
File:Butalbital Acetaminophen and Caffeine NDC 684530170.jpg

Drug Name: Butalbital, Acetaminophen and Caffeine
Ingredient(s): BUTALBITAL[BUTALBITAL], ACETAMINOPHEN[ACETAMINOPHEN], CAFFEINE[CAFFEINE]
Imprint: 170
Dosage: 40 mg
Color(s): Red
Shape: Capsule
Size (mm): 12
Score: 1
NDC:684530170

Drug Label Author: Victory Pharmaceutical

This pill image is provided by the National Library of Medicine's PillBox.

Drug Name: Butalbital, Aspirin, Caffeine and Codeine Phosphate
Ingredient(s): CODEINE PHOSPHATE[CODEINE ANHYDROUS], BUTALBITAL[BUTALBITAL], CAFFEINE[CAFFEINE], ASPIRIN[ASPIRIN]
Imprint: WATSON;3546
Dosage: 40 mg
Color(s): Blue, Yellow
Shape: Capsule
Size (mm): 22
Score: 1
NDC:05913546

Drug Label Author: Watson Laboratories, Inc.

This pill image is provided by the National Library of Medicine's PillBox.

Drug Name: Butalbital, Aspirin and Caffeine
Ingredient(s): BUTALBITAL[BUTALBITAL], ASPIRIN[ASPIRIN], CAFFEINE[CAFFEINE]
Imprint: Westward;785
Dosage: 40 mg
Color(s): White
Shape: Round
Size (mm): 11
Score: 1
NDC:01431785

Drug Label Author: West-ward Pharmaceutical Corp

This pill image is provided by the National Library of Medicine's PillBox.

Drug Name: Butalbital, Aspirin, and Caffeine
Ingredient(s): BUTALBITAL[BUTALBITAL], ASPIRIN[ASPIRIN], CAFFEINE[CAFFEINE]
Imprint: WATSON;3219
Dosage: 40 mg
Color(s): Green, Yellow
Shape: Capsule
Size (mm): 22
Score: 1
NDC:05913219

Drug Label Author: Watson Laboratories, Inc.

This pill image is provided by the National Library of Medicine's PillBox.
File:Butalbital Aspirin and Caffeine Tablets NDC 06032548.jpg

Drug Name: Butalbital, Aspirin and Caffeine Tablets
Ingredient(s): ASPIRIN[ASPIRIN], BUTALBITAL[BUTALBITAL], CAFFEINE[CAFFEINE]
Imprint: West;ward;785
Dosage: 40 mg
Color(s): White
Shape: Round
Size (mm): 7
Score: 1
NDC:06032548

Drug Label Author: Qualitest

This pill image is provided by the National Library of Medicine's PillBox.

Drug Name: Cafergot
Ingredient(s): ERGOTAMINE TARTRATE[ERGOTAMINE], CAFFEINE[CAFFEINE]
Imprint: SZ;183
Dosage: 100 mg
Color(s): Brown
Shape: Round
Size (mm): 10
Score: 1
NDC:07815405

Drug Label Author: Sandoz Inc

This pill image is provided by the National Library of Medicine's PillBox.

Drug Name: Esgic-Plus
Ingredient(s): BUTALBITAL[BUTALBITAL], ACETAMINOPHEN[ACETAMINOPHEN], CAFFEINE[CAFFEINE]
Imprint: FOREST;0372;ESGIC;PLUS
Dosage: 40 mg
Color(s): Red
Shape: Capsule
Size (mm): 22
Score: 1
NDC:04560679

Drug Label Author: Forest Laboratories, Inc.

This pill image is provided by the National Library of Medicine's PillBox.
File:Butalbital acetominophen and caffeine NDC 649800155.jpg

Drug Name: Butalbital, acetominophen and caffeine
Ingredient(s): butalbital[butalbital], acetaminophen[acetaminophen], caffeine[caffeine]
Imprint: CL;026
Dosage: 40 mg
Color(s): White
Shape: Oval
Size (mm): 19
Score: 2
NDC:649800155

Drug Label Author: Rising Pharmaceuticals, Inc.

This pill image is provided by the National Library of Medicine's PillBox.

## References

[edit | edit source]

1. ↑ CoffeeScience.org
2. ↑ caffeine - Definitions from Dictionary.com
3. ↑ 3.0 3.1 Lovett, Richard (24 September 2005). "Coffee: The demon drink?" (fee required). New Scientist (2518). doi:10.1096/fj.03-0496fje.;. Retrieved 2007-11-19. Unknown parameter `|doi_brokendate=` ignored (help); Check date values in: `|date=` (help)
4. ↑ "21 CFR 182.1180". U.S. Code of Federal Regulations. U.S. Office of the Federal Register. 2003-04-01. p. 462. Retrieved 2007-02-16.
5. ↑ 5.0 5.1 Rubin, Rita (2008-01-20). "New studies, different outcomes on caffeine, pregnancy". USA TODAY. Retrieved 2008-02-20.
6. ↑ Frischknecht, P. M. (1986). "Purine alkaloid formation in buds and developing leaflets of Coffea arabica: expression of an optimal defence strategy?" (PDF). Phytochemistry. Journal of the Phytochemical Society of Europe and the Phytochemical Society of North America. 25 (3): 613–616. doi:10.1016/0031-9422(86)88009-8. ISSN: 0031-9422. Unknown parameter `|coauthors=` ignored (help)
7. ↑ Nathanson, J. A. (12 October 1984). "Caffeine and related methylxanthines: possible naturally occurring pesticides". Science. 226 (4671): 184–187. doi:10.1126/science.6207592. PMID 6207592. Check date values in: `|date=` (help)
8. ↑ Baumann, T. W. (1984). "Metabolism and excretion of caffeine during germination of Coffea arabica L." (PDF). Plant and Cell Physiology. Oxford Journals. 25 (8): 1431–1436. ISSN: 0032-0781. Unknown parameter `|coauthors=` ignored (help)
9. ↑ Matissek, R (1997). "Evaluation of xanthine derivatives in chocolate: nutritional and chemical aspects". European Food Research and Technology. 205 (3): 175–184.
10. ↑ 10.0 10.1 "Does Yerba Maté Contain Caffeine or Mateine?#". The Vaults of Erowid. December 2003. Retrieved 2006-08-16.
11. ↑ "PubChem: mateina". National Library of Medicine. Retrieved 2006-08-16.. Generally translated as mateine in articles written in English
12. ↑ "PubChem: guaranine". National Library of Medicine. Retrieved 2006-08-16.
13. ↑ Balentine D. A., Harbowy M. E. and Graham H. N. (1998). G Spiller, ed. Tea: the Plant and its Manufacture; Chemistry and Consumption of the Beverage. Caffeine.
14. ↑ 14.0 14.1 "Caffeine". International Coffee Organization. Retrieved 2006-08-21.
15. ↑ "Coffee and Caffeine FAQ: Does dark roast coffee have less caffeine than light roast?". Retrieved 2007-01-02.
16. ↑ "All About Coffee: Caffeine Level". Jeremiah’s Pick Coffee Co. Retrieved 2007-06-14.
17. ↑ "Caffeine in tea vs. steeping time". September 1996. Retrieved 2006-08-12.
18. ↑ Haskell, C. F. (January 2007). "A double-blind, placebo-controlled, multi-dose evaluation of the acute behavioural effects of guarana in humans". J Psychopharmacol. 21 (1): 65–70. doi:10.1177/0269881106063815. PMID 16533867. Unknown parameter `|coauthors=` ignored (help)
19. ↑ Smit, H. J. (2004 Nov). "Methylxanthines are the psycho-pharmacologically active constituents of chocolate". Psychopharmacology. 176 (3–4): 412–9. doi:10.1007/s00213-004-1898-3. PMID 15549276. Unknown parameter `|coauthors=` ignored (help); Check date values in: `|date=` (help)
20. ↑ "Caffeine Accessories". ThinkGeek, Inc. Retrieved 2007-12-31.
21. ↑ "Does caffeinated soap really work?". Erowid. Retrieved 2008-01-13.
22. ↑ "Altasterol Caffeine Pro+ and mental alertness". Articles base & Alta Care Laboratoires.
23. ↑ Escohotado, Antonio (May 1999). A Brief History of Drugs: From the Stone Age to the Stoned Age. Park Street Press. ISBN 0-89281-826-3. Unknown parameter `|coauthors=` ignored (help)
24. ↑ Chow p. 19–20 (Czech edition); also Arcimovicova p. 9, Evans p. 2 and others
25. ↑ Yu, Lu (October 1995). The Classic of Tea: Origins & Rituals. Ecco Pr; Reissue edition. ISBN 0-88001-416-4.
26. ↑ "Coffee". Encyclopædia Britannica. 1911.
27. ↑ Benjamin, LT Jr (1991 January). "Coca-Cola, caffeine, and mental deficiency: Harry Hollingworth and the Chattanooga trial of 1911". J Hist Behav Sci. 27 (1): 42–55. doi:10.1002/1520-6696(199101)27:1<42::AID-JHBS2300270105>3.0.CO;2-1. PMID 2010614. Unknown parameter `|coauthors=` ignored (help); Check date values in: `|date=` (help)
28. ↑ Jarvis, Gail (May 21 2002). "The Rise and Fall of Cocaine Cola". Retrieved 2006-08-19. Check date values in: `|date=` (help)
29. ↑ 29.0 29.1 Weinberg, BA (January 2001). The World of Caffeine. Routledge. ISBN 0-415-92722-6. Unknown parameter `|coauthors=` ignored (help)
30. ↑ Nobel Prize Presentation Speech by Professor Hj. Théel, President of the Swedish Royal Academy of Sciences on December 10, 1902.
31. ↑ "What's your poison: caffeine". Australian Broadcasting Corporation. 1997. Retrieved 2006-08-20.
32. ↑ Nehlig, A (1992 May-Aug). "Caffeine and the central nervous system: Mechanisms of action, biochemical, metabolic, and psychostimulant effects". Brain Res Rev. 17 (2): 139–70. doi:10.1016/0165-0173(92)90012-B. PMID 1356551. Unknown parameter `|coauthors=` ignored (help); Check date values in: `|date=` (help)
33. ↑ 33.0 33.1 Bolton, Ph.D., Sanford (1981). "Caffeine: Psychological Effects, Use and Abuse". Orthomolecular Psychiatry. 10 (3): 202–211. doi:10.1152/physrev.00004.2004<br. Retrieved 2006-08-12. Unknown parameter `|doi_brokendate=` ignored (help); Unknown parameter `|coauthors=` ignored (help)
34. ↑ Newton, R (1981). "Plasma and salivary pharmacokinetics of caffeine in man". European Journal of Clinical Pharmacology. 21 (1): 45&ndash, 52. doi:10.1007/BF00609587. PMID 7333346. Unknown parameter `|coauthors=` ignored (help)
35. ↑ Meyer, FP (1991). "Time course of inhibition of caffeine elimination in response to the oral depot contraceptive agent Deposiston. Hormonal contraceptives and caffeine elimination". Zentralbl Gynakol. 113 (6): 297&ndash, 302. PMID 2058339. Unknown parameter `|coauthors=` ignored (help)
36. ↑ Ortweiler, W (1985). "Determination of caffeine and metamizole elimination in pregnancy and after delivery as an in vivo method for characterization of various cytochrome p-450 dependent biotransformation reactions". Biomed Biochim Acta. 44 (7–8): 1189&ndash, 99. PMID 4084271. Unknown parameter `|coauthors=` ignored (help)
37. ↑ Bolton, Ph.D., Sanford (1981). "Caffeine: Psychological Effects, Use and Abuse". Orthomolecular Psychiatry. 10 (3): 202&ndash, 211. doi:10.1152/physrev.00004.2004<br. Retrieved 2006-08-14. Unknown parameter `|doi_brokendate=` ignored (help); Unknown parameter `|coauthors=` ignored (help)
38. ↑ Springhouse (January 1 2005). Physician's Drug Handbook; 11th edition. Lippincott Williams & Wilkins. ISBN 1-58255-396-3. Check date values in: `|date=` (help)
39. ↑ "Caffeine". The Pharmacogenetics and Pharmacogenomics Knowledge Base. Retrieved 2006-08-14.
40. ↑ Fisone G, G (April 2004). "Caffeine as a psychomotor stimulant: mechanism of action". Cell Mol Life Sci. 61 (7–8): 857–72. doi:10.1007/s00018-003-3269-3. PMID 15095008. Unknown parameter `|coauthors=` ignored (help)
41. ↑ 41.0 41.1 41.2 Fredholm, B.B., 1995. Adenosine, adenosine receptors and the actions of caffeine. Pharmac. Toxic. 76, pp. 93-101
42. ↑ Rathbone, MP et al, 1999. Trophic effects of purines in neurons and glial cells. Progress in Neurobiology. 59, pp. 663–690
43. ↑ Mitchell, J.B., Lupica, C.R. and Dunwiddie, T.V., 1993. Activity-dependent release of endogenous adenosine modulates synaptic responses in the rat hippocampus. Neuroscience 13, pp. 3439–3447.
44. ↑ Caulia O., Morellia M., 2005. Caffeine and the dopaminergic system. Behavioural Pharmacology. 16, pp. 63–77.
45. ↑ Caffeine induces dopamine and glutamate release in the shell of the nucleus accumbens. Marcello Solinas, Sergi Ferré, Zhi-Bing You, Marzena Karcz-Kubicha, Patrizia Popoli, Steven R. Goldberg. The Journal of Neuroscience, August 1, 2002, 22(15):6321-6324.
46. ↑ Nehlig A, (March 1999). "Are we dependent upon coffee and caffeine? A review on human and animal data". Neurosci Biobehav Rev. 23 (4): 563–76. doi:10.1016/S0149-7634(98)00050-5. line feed character in `|title=` at position 43 (help)
47. ↑ Stephen G. Holtzman, (1983-08-22). "Complete, reversible, drug-specific tolerance to stimulation of locomotor activity by caffeine". Life Sciences. 33 (8): 779–787. doi:10.1016/0024-3205(83)90784-1.
48. ↑ Stephen G. Holtzman, (August 1988). "Complete, reversible, drug-specific tolerance to stimulation of locomotor activity by caffeine". Pharmacology Biochemistry and Behavior. 30 (4): 809–815. doi:10.1016/0091-3057(88)90104-9.
49. ↑ Graham T, Rush J, van Soeren M (1994). "Caffeine and exercise: metabolism and performance". Can J Appl Physiol. 19 (2): 111–38. PMID 8081318.CS1 maint: Multiple names: authors list (link)
50. ↑ 50.0 50.1 Fredholm B, Bättig K, Holmén J, Nehlig A, Zvartau E (1999). "Actions of caffeine in the brain with special reference to factors that contribute to its widespread use". Pharmacol Rev. 51 (1): 83–133. doi:10.1152/physrev.00004.2004. PMID 10049999.CS1 maint: Multiple names: authors list (link) Full text
51. ↑ Verkhratsky A. (2005). "Physiology and Pathophysiology of the Calcium Store in the Endoplasmic Reticulum of Neurons". Physiol. Rev. 85 (1): 201–279. doi:10.1152/physrev.00004.2004. PMID 15618481.
52. ↑ Dews, P.B. (1984). Caffeine: Perspectives from Recent Research. Berlin: Springer-Valerag. ISBN 978-0387135328.
53. ↑ Ivy, JL (1979 Spring). "Influence of caffeine and carbohydrate feedings on endurance performance". Med Sci Sports. 11 (1): 6–11. PMID 481158. Unknown parameter `|coauthors=` ignored (help); Check date values in: `|date=` (help)
54. ↑ Graham, TE (1991 December). "Performance and metabolic responses to a high caffeine dose during prolonged exercise". J Appl Physiol. 71 (6): 2292–8. PMID 1778925. Unknown parameter `|coauthors=` ignored (help); Check date values in: `|date=` (help)
55. ↑ Trice, I (March 1995). "Effects of caffeine ingestion on exercise-induced changes during high-intensity, intermittent exercise". Int J Sport Nutr. 5 (1): 37–44. doi:10.1152/physrev.00004.2004. PMID 7749424. Unknown parameter `|coauthors=` ignored (help)
56. ↑ "Caffeine (Systemic)". MedlinePlus. 2000-05-25. Archived from the original on 2007-02-23. Retrieved 2006-08-12.
57. ↑ Schmidt, B (May 18 2006). "Caffeine therapy for apnea of prematurity". N Engl J Med. 354 (20): 2112–21. doi:10.1056/NEJMoa054065. PMID 16707748. Unknown parameter `|coauthors=` ignored (help); Check date values in: `|date=` (help)
58. ↑ Schmidt, B (November 8 2007). "Long-Term Effects of Caffeine Therapy for Apnea of Prematurity". N Engl J Med. 357 (19): 1893–1902. doi:10.1056/NEJMoa073679. PMID 17989382. Unknown parameter `|coauthors=` ignored (help); Check date values in: `|date=` (help)
59. ↑ Schmidt, B (2005). "Methylxanthine Therapy for Apnea of Prematurity: Evaluation of Treatment Benefits and Risks at Age 5 Years in the International Caffeine for Apnea of Prematurity (CAP) Trial". Neonatology. 88 (3): 208–213. doi:10.1159/000087584.
60. ↑ Noever, R., J. Cronise, and R. A. Relwani. 1995. Using spider-web patterns to determine toxicity. NASA Tech Briefs 19(4):82. Published in New Scientist magazine, 27 April 1995.
61. ↑ Fecal incontinence, NIH
62. ↑ Diagnostic and Statistical Manual of Mental Disorders, fourth Edition. American Psychiatric Association. 1994. ISBN 0-89042-062-9.
63. ↑ Invalid `<ref>` tag; no text was provided for refs named `EofMD`
64. ↑ "Caffeine overdose". MedlinePlus. 2006-04-04. Retrieved 2006-08-14.
65. ↑ Kamijo, Y (1999 December). "Severe rhabdomyolysis following massive ingestion of oolong tea: caffeine intoxication with coexisting hyponatremia". Veterinary and Human Toxicology. 41 (6): 381–3. doi:10.1152/physrev.00004.2004. PMID 10592946. Unknown parameter `|coauthors=` ignored (help); Check date values in: `|date=` (help)
66. ↑ Peters, Josef M. (1967). "Factors Affecting Caffeine Toxicity: A Review of the Literature". The Journal of Clinical Pharmacology and the Journal of New Drugs (7): 131–141.
67. ↑ Kerrigan, S (October 4, 2005). "Fatal caffeine overdose: two case reports" (reprint). Forensic Sci Int. 153 (1): 67–69. doi:10.1016/j.forsciint.2005.04.016. PMID 15935584. Unknown parameter `|coauthors=` ignored (help); Check date values in: `|date=` (help)
68. ↑ Holmgren, P (January 6, 2004). "Caffeine fatalities — four case reports" (reprint). Forensic Sci Int. 139 (1): 71–73. doi:10.1016/j.forsciint.2003.09.019. PMID 14687776. Unknown parameter `|coauthors=` ignored (help); Check date values in: `|date=` (help)
69. ↑ Walsh, I (December 1987). "Near-fatal caffeine intoxication treated with peritoneal dialysis". Pediatr Emerg Care. 3 (4): 244–9. doi:10.1152/physrev.00004.2004. PMID 3324064. Unknown parameter `|coauthors=` ignored (help)
70. ↑ Mrvos, RM (December 1989). "Massive caffeine ingestion resulting in death". Vet Hum Toxicol. 31 (6): 571–2. doi:10.1152/physrev.00004.2004. PMID 2617841. Unknown parameter `|coauthors=` ignored (help)
71. ↑ 71.0 71.1 71.2 71.3 71.4 71.5 71.6 Diagnostic and statistical manual of mental disorders : DSM-5. Washington, D.C: American Psychiatric Association. 2013. ISBN 0890425558.
72. ↑ "Caffeine Content of Food and Drugs". Nutrition Action Health Newsletter. Center for Science in the Public Interest. December 1996. Archived from the original on 2007-06-14. Retrieved 2006-08-22.
73. ↑ "Caffeine Content of Beverages, Foods, & Medications". The Vaults of Erowid. July 7 2006. Retrieved 2006-08-22. Check date values in: `|date=` (help)
74. ↑ 74.0 74.1 Green, RM (January 1986). "Chronic caffeine ingestion sensitizes the A1 adenosine receptor-adenylate cyclase system in rat cerebral cortex". J Clin Invest. 77 (1): 222–227. doi:10.1172/JCI112280. PMID 3003150. Unknown parameter `|coauthors=` ignored (help)
75. ↑ Holtzman SG, Mante S, Minneman KP (1991). "Role of adenosine receptors in caffeine tolerance". J. Pharmacol. Exp. Ther. 256 (1): 62–8. PMID 1846425.CS1 maint: Multiple names: authors list (link)
76. ↑ Caffeine - A Drug of Abuse?
77. ↑ Information About Caffeine Dependence
78. ↑ Health risks of Stimulants, healthandgoodness.com
79. ↑ Juliano, L M (2004-09-21). "A critical review of caffeine withdrawal: empirical validation of symptoms and signs, incidence, severity, and associated features". Psychopharmacology. 176 (1): 1–29. doi:10.1007/s00213-004-2000-x.
80. ↑ Sawynok, J (January 1995). "Pharmacological rationale for the clinical use of caffeine". Drugs. 49 (1): 37–50. doi:10.1152/physrev.00004.2004. PMID 7705215.
81. ↑ "Headache Triggers: Caffeine". WebMD. June 2004. Retrieved 2006-08-14.
82. ↑ Invalid `<ref>` tag; no text was provided for refs named `BJoA`
83. ↑ Effects of caffeine on Parkinson's disease, Harvard research
84. ↑ "New Findings About Parkinson's Disease: Coffee and Hormones Don't Mix". National Institute of Neurological Disorders and Stroke.
85. ↑ Han, ME; Park, KH; Baek, SY; Kim, BS; Han, JB; Kim, SO; Oh (2007-03-26), "Inhibitory effects of caffeine on hippocampal neurogenesis and function.", Biochem Biophys Res Commun., Korea: Epub, 18 (4): 976–80, ISSN 0343-6993 Unknown parameter `|a-uthor3-link=` ignored (help); More than one of `|author4=` and `|last4=` specified (help); Check date values in: `|date=` (help)CS1 maint: Date and year (link) .
86. ↑ "Caffeine clue to better memory". BBC News. 1999-10-12.
87. ↑ "Caffeine Boosts Short-Time Memory".
88. ↑ Lesk VE, Womble SP. (2004). "Caffeine, priming, and tip of the tongue: evidence for plasticity in the phonological system". Behavioral Neuroscience. 118 (2): 453&ndash, 61. doi:10.1037/0735-7044.118.3.453. PMID 15174922.
89. ↑ Greenberg, J.A. (2007). "Caffeinated beverage intake and the risk of heart disease mortality in the elderly: a prospective analysis". Am J Clin Nutr. 85 (2): 392–398. PMID 17284734. Unknown parameter `|coauthors=` ignored (help); Unknown parameter `|month=` ignored (help)
90. ↑ "Fact or fiction: Common diet myths dispelled". MSNBC. December 2006. Retrieved 2007-05-10.
91. ↑ "Caffeine and Your Child". KidsHealth. January 2005. Retrieved 2007-05-10.
92. ↑ Agency issues caffeine advice to pregnant women
93. ↑ Study: Caffeine may boost miscarriage risk
94. ↑ Kaiser Permanente Study Shows Newer, Stronger Evidence that Caffeine During Pregnancy Increases Miscarriage Risk
95. ↑ Simon Tilling. "Crystalline Caffeine". Bristol University.
96. ↑ Ted Wilson, Norman J. Temple (2004). Beverages in Nutrition and Health. Humana Press. p. 172\. ISBN 1588291731.
97. ↑ 97.0 97.1 Senese, Fred (2005-09-20). "How is coffee decaffeinated?". General Chemistry Online. Retrieved 2006-08-21.
98. ↑ "Voices of Faith: April 12, 2008". Retrieved 2008-05-13.

### General information

[edit | edit source]

* How Stuff Works: "How Caffeine Works"
* Erowid Caffeine Vaults
* National Geographic January 2005: Caffeine
* Caffeine Zone: Social and Medical info on caffeine and its effects.
* #caffeine! The Caffeine Information Archive
* Naked Scientists Online: Why do plants make caffeine?
* The Physician and Sportsmedicine: Caffeine: A User's Guide
* The Consumers Union Report on Licit and Illicit Drugs, Caffeine-Part 1 Part 2
* Coffee: A Little Really Does Go a Long Way, NPR, September 28, 2006
* Does coffee really give you a buzz? by John Triggs in the Daily Express April 17 2007
* Caffeine: ChemSub Online

### News

[edit | edit source]

* Alcohol and Drugs History Society: Caffeine news page
* National Post: Caffeine linked to psychiatric disorders
* Caffeine Withdrawal Recognized as a Disorder

### Health

[edit | edit source]

* Is Caffeine a Health Hazard?
* eMedicine Caffeine-Related Psychiatric Disorders
* The Effects Of Caffeine Addiction
* Caffeine: Psychological Effects, Use & Abuse
* Protects brain from Alzheimer's?

## Appendix

[edit | edit source]

### Relative content: comparison of different sources

[edit | edit source]

Caffeine equivalents[1][2]

In general, each of the following contains approximately 200 milligrams of caffeine:

* One 200 milligram caffeine pill
* One 12 fluid ounce cup of regular Starbucks coffee (355 millilitres)
* One and one quarter 16 fluid ounce cans of Monster Energy (590 millilitres)
* One and a half pounds of milk chocolate[a] (680 grams)
* Two 8 fluid ounce containers of regular coffee (470 millilitres)
* 1/2 tube of Spazzstick Caffeinated Lip Balm
* Two Foosh Energy Mints
* Two Buzz Bites Chocolate Energy Chews
* Two and a half 10 fluid ounce bottles of Bawls caffeinated drink (740 millilitres)
* Three 6 fluid ounce cups of black tea (0.54 litres) (70 mg per 6 oz cup[1])
* Three standard Excedrin pills
* Three 8 fluid ounce cups of Red Bull energy drink (710 millilitres)
* Four 8 fluid ounce cups of Vault energy drink (1.0 litre)
* Five 1 fluid ounce shots of espresso from robusta beans (150 millilitres)
* Five 8 fluid ounce cups of Mountain Dew (1.2 litres)
* Five 12 fluid ounce cans of typical soda pop (1.8 litres) (variable)
* Eight and a half 8 fluid ounce cups of Coca-Cola Classic (68 fl oz is approximately 2.0 litres)
* Ten 8 fluid ounce cups of green tea (2.4 litres)
* Fifty 8 fluid ounce cups of decaffeinated coffee (12 litres)

Notes:

a. Chocolate and other products of cacao contain substantial quantities of other caffeine-like chemicals (especially theobromine) as well as actual caffeine. The properties of these substances are in at least some respects very similar to caffeine.

Template:Coffee Template:Stimulants Template:Psychostimulants, agents used for ADHD and nootropics Template:Featured article

ar:كافايين bg:Кофеин ca:Cafeïna cs:Kofein cy:Caffein da:Koffein de:Coffein et:Kofeiin eo:Kafeino ga:Caiféin gl:Cafeína ko:카페인 hr:Kofein io:Kafeino id:Kafein is:Kaffín it:Caffeina he:קפאין kn:ಕೆಫೀನ್ lv:Kofeīns lt:Kofeinas hu:Koffein ms:Kafeina nl:Cafeïne no:Koffein nn:Koffein sq:Kafeina simple:Caffeine sk:Kofeín sl:Kofein sr:Кофеин sh:Kofein fi:Kofeiini sv:Koffein ta:காஃவீன் th:คาเฟอีน uk:Кофеїн yi:קאפין

1. ↑ 1.0 1.1 Invalid `<ref>` tag; no text was provided for refs named `Caffeine Content of Food and Drugs`
2. ↑ Invalid `<ref>` tag; no text was provided for refs named `Erowid Caffeine Content`