Ligase chain reaction

WikiDoc Resources for Ligase chain reaction

Articles

Most recent articles on Ligase chain reaction

Most cited articles on Ligase chain reaction

Review articles on Ligase chain reaction

Articles on Ligase chain reaction in N Eng J Med, Lancet, BMJ

Media

Powerpoint slides on Ligase chain reaction

Images of Ligase chain reaction

Photos of Ligase chain reaction

Podcasts & MP3s on Ligase chain reaction

Videos on Ligase chain reaction

Evidence Based Medicine

Cochrane Collaboration on Ligase chain reaction

Bandolier on Ligase chain reaction

TRIP on Ligase chain reaction

Clinical Trials

Ongoing Trials on Ligase chain reaction at Clinical Trials.gov

Trial results on Ligase chain reaction

Clinical Trials on Ligase chain reaction at Google

Guidelines / Policies / Govt

US National Guidelines Clearinghouse on Ligase chain reaction

NICE Guidance on Ligase chain reaction

NHS PRODIGY Guidance

FDA on Ligase chain reaction

CDC on Ligase chain reaction

Books

Books on Ligase chain reaction

News

Ligase chain reaction in the news

Be alerted to news on Ligase chain reaction

News trends on Ligase chain reaction

Commentary

Blogs on Ligase chain reaction

Definitions

Definitions of Ligase chain reaction

Patient Resources / Community

Patient resources on Ligase chain reaction

Discussion groups on Ligase chain reaction

Patient Handouts on Ligase chain reaction

Directions to Hospitals Treating Ligase chain reaction

Risk calculators and risk factors for Ligase chain reaction

Healthcare Provider Resources

Symptoms of Ligase chain reaction

Causes & Risk Factors for Ligase chain reaction

Diagnostic studies for Ligase chain reaction

Treatment of Ligase chain reaction

Continuing Medical Education (CME)

CME Programs on Ligase chain reaction

International

Ligase chain reaction en Espanol

Ligase chain reaction en Francais

Business

Ligase chain reaction in the Marketplace

Patents on Ligase chain reaction

Experimental / Informatics

List of terms related to Ligase chain reaction

Editor-In-Chief: C. Michael Gibson, M.S., M.D. [1]

## Contents

* 1 Overview
* 2 Target conditions
* 3 References
* 4 General reading

## Overview

[edit | edit source]

The ligase chain reaction (LCR) is a nucleic acid amplification technique. While the better-known PCR carries out the amplification by polymerizing nucleotides, LCR instead amplifies the nucleic acid used as the probe. For each of the two DNA strands, two partial probes are ligated to form the actual one; thus, LCR uses two enzymes: a DNA polymerase and a DNA ligase. Each cycle results in a doubling of the target nucleic acid molecule. A key advantage of LCR is greater specificity as compared to PCR.[1].

## Target conditions

[edit | edit source]

It has been widely used for the detection of single base mutations, as in genetic diseases.[2]

LCR and PCR may be used to detect gonorrhea and chlamydia, and may be performed on first-catch urine samples, providing easy collection and a large yield of organisms. Endogenous inhibitors limit the sensitivity, but if this effect could be eliminated, LCR and PCR would have clinical advantages over any other methods of diagnosing gonorrhea and chlamydia.[3]

## References

[edit | edit source]

1. ↑ Wiedmann M, Wilson WJ, Czajka J, Luo J, Barany F, Batt CA. "Ligase chain reaction (LCR)--overview and applications."  PCR Methods and Applications 1994 Feb;3(4):S51-64 PMID: 8173509
2. ↑ Barany F. "Genetic disease detection and DNA amplification using cloned thermostable ligase." Proc Natl Acad Sci U S A. 1991 Jan 1;88(1):189-93. PMID: 1986365
3. ↑ New diagnostic tests for gonorrhea and chlamydia Gregory J. Locksmith MD. Department of Obstetrics and Gynecology, University of Florida College of Medicine, Gainesville, Florida, USA. Available online 13 February 1998.

## General reading

[edit | edit source]

* Walker, J. M., & Rapley, R. (2005). Medical biomethods handbook. Totowa, N.J.: Humana Press. ISBN 9781592598700

de:Ligase-Kettenreaktion it:Reazione a catena della ligasi

Template:WH Template:WS