SETMAR

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Identifiers
Aliases|
External IDs| GeneCards: [1]
Orthologs
Species| Human| Mouse
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Location (UCSC)| n/a| n/a
PubMed search| n/a| n/a
Wikidata
| View/Edit Human|

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Histone-lysine N-methyltransferase SETMAR is an enzyme that in humans is encoded by the SETMAR gene.[1][2]

## Model organisms

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Setmar knockout mouse phenotype. SETMAR is found only in anthropoid primates. Therefore, the knockout mouse is not for SETMAR but only the SET domain of this chimeric fusion protein.  Characteristic | Phenotype

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Homozygote viability | Normal
Homozygous Fertility | Normal
Body weight | Normal
Anxiety | Normal
Neurological assessment | Normal
Grip strength | Normal
Hot plate | Normal
Dysmorphology | Normal
Indirect calorimetry | Normal
Glucose tolerance test | Normal
Auditory brainstem response | Normal
DEXA | Normal
Radiography | Normal
Body temperature | Normal
Eye morphology | Abnormal[3]
Clinical chemistry | Normal
Plasma immunoglobulins | Normal
Haematology | Normal
Peripheral blood lymphocytes | Abnormal[4]
Micronucleus test | Normal
Heart weight | Normal
Skin Histopathology | Normal
Brain histopathology | Normal
Salmonella infection | Normal[5]
Citrobacter infection | Normal[6]
All tests and analysis from[7][8]

Model organisms have been used in the study of SETMAR function. A conditional knockout mouse line, called Setmartm1a(EUCOMM)Wtsi[9][10] was generated as part of the International Knockout Mouse Consortium program — a high-throughput mutagenesis project to generate and distribute animal models of disease to interested scientists.[11][12][13]

Male and female animals underwent a standardized phenotypic screen to determine the effects of deletion.[7][14] Twenty five tests were carried out on mutant mice and two significant abnormalities were observed.[7] Homozygous mutant animals of both sex had abnormal retinal pigmentation and morphology, while males also had atypical peripheral blood lymphocyte parameters.[7]

## References

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1. ↑ Robertson HM; Zumpano KL (Feb 1998). "Molecular evolution of an ancient mariner transposon, Hsmar1, in the human genome". Gene. 205 (1–2): 203–17. doi:10.1016/S0378-1119(97)00472-1. PMID 9461395.
2. ↑ "Entrez Gene: SETMAR SET domain and mariner transposase fusion gene".
3. ↑ "Eye morphology data for Setmar". Wellcome Trust Sanger Institute.
4. ↑ "Peripheral blood lymphocytes data for Setmar". Wellcome Trust Sanger Institute.
5. ↑ "Salmonella infection data for Setmar". Wellcome Trust Sanger Institute.
6. ↑ "Citrobacter infection data for Setmar". Wellcome Trust Sanger Institute.
7. ↑ 7.0 7.1 7.2 7.3 Gerdin, AK (2010). "The Sanger Mouse Genetics Programme: High throughput characterisation of knockout mice". Acta Ophthalmologica. 88: 925–7. doi:10.1111/j.1755-3768.2010.4142.x.
8. ↑ Mouse Resources Portal, Wellcome Trust Sanger Institute.
9. ↑ "International Knockout Mouse Consortium".
10. ↑ "Mouse Genome Informatics".
11. ↑ Skarnes, W. C.; Rosen, B.; West, A. P.; Koutsourakis, M.; Bushell, W.; Iyer, V.; Mujica, A. O.; Thomas, M.; Harrow, J.; Cox, T.; Jackson, D.; Severin, J.; Biggs, P.; Fu, J.; Nefedov, M.; De Jong, P. J.; Stewart, A. F.; Bradley, A. (2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature. 474 (7351): 337–342. doi:10.1038/nature10163. PMC 3572410. PMID 21677750.
12. ↑ Dolgin, Elie (2011). "Mouse library set to be knockout". Nature. 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718.
13. ↑ International Mouse Knockout Consortium; Collins, FS; Rossant, J; Wurst, W (2007). "A Mouse for All Reasons". Cell. 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247.
14. ↑ van der Weyden L; White JK; Adams DJ; Logan DW (2011). "The mouse genetics toolkit: revealing function and mechanism". Genome Biol. 12 (6): 224. doi:10.1186/gb-2011-12-6-224. PMC 3218837. PMID 21722353.

## Further reading

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* Berry R, Stevens TJ, Walter NA, et al. (1995). "Gene-based sequence-tagged-sites (STSs) as the basis for a human gene map". Nat. Genet. 10 (4): 415–23. doi:10.1038/ng0895-415. PMID 7670491.
* Maruyama K; Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
* Andersson B, Wentland MA, Ricafrente JY, et al. (1996). "A "double adaptor" method for improved shotgun library construction". Anal. Biochem. 236 (1): 107–13. doi:10.1006/abio.1996.0138. PMID 8619474.
* Yu W, Andersson B, Worley KC, et al. (1997). "Large-scale concatenation cDNA sequencing". Genome Res. 7 (4): 353–8. doi:10.1101/gr.7.4.353. PMC 139146. PMID 9110174.
* Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
* Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
* Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
* Lee SH, Oshige M, Durant ST, et al. (2006). "The SET domain protein Metnase mediates foreign DNA integration and links integration to nonhomologous end-joining repair". Proc. Natl. Acad. Sci. U.S.A. 102 (50): 18075–80. Bibcode:2005PNAS..10218075L. doi:10.1073/pnas.0503676102. PMC 1312370. PMID 16332963.
* Cordaux R; Udit S; Batzer MA; Feschotte C (2006). "Birth of a chimeric primate gene by capture of the transposase gene from a mobile element". Proc. Natl. Acad. Sci. U.S.A. 103 (21): 8101–6. Bibcode:2006PNAS..103.8101C. doi:10.1073/pnas.0601161103. PMC 1472436. PMID 16672366.
* Keravala A, Liu D, Lechman ER, et al. (2007). "Hyperactive Himar1 transposase mediates transposition in cell culture and enhances gene expression in vivo". Hum. Gene Ther. 17 (10): 1006–18. doi:10.1089/hum.2006.17.1006. PMID 16989604.
* Liu D, Bischerour J, Siddique A, et al. (2007). "The human SETMAR protein preserves most of the activities of the ancestral Hsmar1 transposase". Mol. Cell. Biol. 27 (3): 1125–32. doi:10.1128/MCB.01899-06. PMC 1800679. PMID 17130240.
* Miskey C, Papp B, Mátés L, et al. (2007). "The ancient mariner sails again: transposition of the human Hsmar1 element by a reconstructed transposase and activities of the SETMAR protein on transposon ends". Mol. Cell. Biol. 27 (12): 4589–600. doi:10.1128/MCB.02027-06. PMC 1900042. PMID 17403897.
* Roman Y, Oshige M, Lee YJ, et al. (2007). "Biochemical characterization of a SET and transposase fusion protein, Metnase: its DNA binding and DNA cleavage activity". Biochemistry. 46 (40): 11369–76. doi:10.1021/bi7005477. PMC 3374406. PMID 17877369.