ADA (buffer)

ADA

Names
Systematic IUPAC name

2,2′-[(2-amino-2-oxoethyl)azanediyl]diacetic acid

Other names

ADA, N-(2-acetamido)iminodiacetic acid, N-(carbamoylmethyl)iminodiacetic acid

Identifiers

CAS Number

* 26239-55-4

3D model (JSmol)

* Interactive image

ECHA InfoCard | 100.043.194
EC Number |

* 247-530-0

PubChem CID

* 117765

UNII |

* 5C4R3O704E

CompTox Dashboard (EPA)

* DTXSID4067191

InChI

* InChI=1S/C6H10N2O5/c7-4(9)1-8(2-5(10)11)3-6(12)13/h1-3H2,(H2,7,9)(H,10,11)(H,12,13)

Key: QZTKDVCDBIDYMD-UHFFFAOYSA-N

SMILES

* C(C(=O)N)N(CC(=O)O)CC(=O)O

Properties

Chemical formula

| C6H10N2O5
Molar mass | 190.155 g/mol
Acidity (pKa)  | 6.6
Hazards
Occupational safety and health (OHS/OSH):

Main hazards

| Irritant
GHS labelling:

Signal word

| Warning

Hazard statements

| H302, H315, H319, H335

Precautionary statements

| P261, P264, P270, P271, P280, P301+P312, P302+P352, P304+P340, P305+P351+P338, P312, P321, P330, P332+P313, P337+P313, P362, P403+P233, P405, P501
Flash point | Non-flammable

Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).

Infobox references

Chemical compound

ADA is a zwitterionic organic chemical buffering agent; one of Good's buffers. It has a useful pH range of 6.0-7.2 in the physiological range, making it useful for cell culture work. It has a pKa of 6.6 with ΔpKa/°C of -0.011 and is most often prepared in 1 M NaOH where it has a solubility of 160 mg/mL.

ADA has been used in protein-free media for chicken embryo fibroblasts, as a chelating agent for H+, Ca2+, and Mg2+, and for isoelectric focusing in immobilized pH gradients. Its effects on dog kidney Na+/K+-ATPase and rat brain GABA receptors have also been studied. ADA does, however, alter coloring in bicinchoninic acid assays.[1]

## References

[edit]

1. ^ "ADA" (PDF). Sigma-Aldrich.

*[CID]: Compound ID
*[EPA]: U.S. Environmental Protection Agency
*[H302]: H302: Harmful if swallowed
*[H315]: H315: Causes skin irritation
*[H319]: H319: Causes serious eye irritation
*[H335]: H335: May cause respiratory irritation
*[P261]: P261: Avoid breathing dust/fume/gas/mist/vapours/spray.
*[P264]: P264: Wash ... thoroughly after handling.
*[P270]: P270: Do not eat, drink or smoke when using this product.
*[P271]: P271: Use only outdoors or in a well-ventilated area.
*[P280]: P280: Wear protective gloves/protective clothing/eye protection/face protection.
*[P301+P312]: P301+P312: IF SWALLOWED: Call a POISON CENTER or doctor/physician if you feel unwell.
*[P302+P352]: P302+P352: IF ON SKIN: Wash with soap and water.
*[P304+P340]: P304+P340: IF INHALED: Remove victim to fresh air and keep at rest in a position comfortable for breathing.
*[P305+P351+P338]: P305+P351+P338: IF IN EYES: Rinse continuously with water for several minutes. Remove contact lenses if present and easy to do. Continue rinsing.
*[P312]: P312: Call a POISON CENTER or doctor/physician if you feel unwell.
*[P321]: P321: Specific treatment (see ... on this label).
*[P330]: P330: Rinse mouth.
*[P332+P313]: P332+P313: If skin irritation occurs: Get medical advice/attention.
*[P337+P313]: P337+P313: If eye irritation persists: Get medical advice/attention.
*[P362]: P362: Take off contaminated clothing.
*[P403+P233]: P403+P233: Store in a well ventilated place. Keep container tightly closed.
*[P405]: P405: Store locked up.
*[P501]: P501: Dispose of contents/container to ...