Gate (cytometry)

Flow cytometry gating into main categories of blood cells by side scatter and CD45.

A gate in cytometry is a set of value limits (boundaries) that serve to isolate a specific group of cytometric events from a large set. Gates can be defined by discrimination analysis, or can simply be drawn around a given set of data points on a printout and then converted to a computer-useful form. Gates can be implemented with a physical blinder. Gates may be used either to selectively gather data or to segregate data for analysis.

## Division

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Gates are divided mathematically into inclusive gates and exclusive gates. Inclusive gates select data that falls within the limits set, while exclusive gates select data that falls outside the limits.

## Live gate

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A live gate is a term used for a process that prevents the acquisition by the computer of non-selected data from the flow cytometer.

## External links

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* "General Flow Cytometry Glossary and Cell Cycle Analysis Terminology" The Janis V. Giorgi Flow Cytometry Laboratory, UCLA
* Osborne, G. W. (2000) "Regions and Gates" Flow Cytometry Software Workshop: 2000, page 3