Germacrene A alcohol dehydrogenase

Identifiers
EC no.| 1.1.1.314
Databases
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KEGG| KEGG entry
MetaCyc| metabolic pathway
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PMC| articles
PubMed| articles
NCBI| proteins

Germacrene A alcohol dehydrogenase (EC 1.1.1.314) is an enzyme with systematic name germacra-1(10),4,11(13)-trien-12-ol:NADP+ oxidoreductase.[1][2] This enzyme catalyses the following chemical reaction

germacra-1(10),4,11(13)-trien-12-ol + 2 NADP+ \+ H2O  ⇌ {\displaystyle \rightleftharpoons } germacra-1(10),4,11(13)-trien-12-oate + 2 NADPH + 3 H+ (overall reaction)
(1a) germacra-1(10),4,11(13)-trien-12-ol + NADP+ ⇌ {\displaystyle \rightleftharpoons } germacra-1(10),4,11(13)-trien-12-al + NADPH + H+
(1b) germacra-1(10),4,11(13)-trien-12-al + NADP+ \+ H2O  ⇌ {\displaystyle \rightleftharpoons } germacra-1(10),4,11(13)-trien-12-oate + NADPH + 2 H+

In Lactuca sativa this enzyme is a multifunctional enzyme with EC 1.14.13.123.

## References

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1. ^ de Kraker JW, Franssen MC, Dalm MC, de Groot A, Bouwmeester HJ (April 2001). "Biosynthesis of germacrene A carboxylic acid in chicory roots. Demonstration of a cytochrome P450 (+)-germacrene a hydroxylase and NADP+-dependent sesquiterpenoid dehydrogenase(s) involved in sesquiterpene lactone biosynthesis". Plant Physiology. 125 (4): 1930–40\. doi:10.1104/pp.125.4.1930. PMC 88848. PMID 11299372.1930-40&rft.date=2001-04&rft_id=https://www.ncbi.nlm.nih.gov/pmc/articles/PMC88848#id-name=PMC&rft_id=info:pmid/11299372&rft_id=info:doi/10.1104/pp.125.4.1930&rft.aulast=de+Kraker&rft.aufirst=JW&rft.au=Franssen,+MC&rft.au=Dalm,+MC&rft.au=de+Groot,+A&rft.au=Bouwmeester,+HJ&rft_id=https://www.ncbi.nlm.nih.gov/pmc/articles/PMC88848&rfr_id=info:sid/en.wikipedia.org:Germacrene+A+alcohol+dehydrogenase" class="Z3988">
2. ^ Nguyen DT, Göpfert JC, Ikezawa N, Macnevin G, Kathiresan M, Conrad J, Spring O, Ro DK (May 2010). "Biochemical conservation and evolution of germacrene A oxidase in asteraceae". The Journal of Biological Chemistry. 285 (22): 16588–98\. doi:10.1074/jbc.M110.111757. PMC 2878029. PMID 20351109.16588-98&rft.date=2010-05&rft_id=https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2878029#id-name=PMC&rft_id=info:pmid/20351109&rft_id=info:doi/10.1074/jbc.M110.111757&rft.aulast=Nguyen&rft.aufirst=DT&rft.au=Göpfert,+JC&rft.au=Ikezawa,+N&rft.au=Macnevin,+G&rft.au=Kathiresan,+M&rft.au=Conrad,+J&rft.au=Spring,+O&rft.au=Ro,+DK&rft_id=https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2878029&rfr_id=info:sid/en.wikipedia.org:Germacrene+A+alcohol+dehydrogenase" class="Z3988">

## External links

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* Germacrene+A+alcohol+dehydrogenase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)

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Oxidoreductases: alcohol oxidoreductases (EC 1.1)

1.1.1: NAD/NADP acceptor|

* 3-hydroxyacyl-CoA dehydrogenase
* 3-hydroxybutyryl-CoA dehydrogenase
* Alcohol dehydrogenase
* Aldo-keto reductase
* 1A1
* 1B1
* 1B10
* 1C1
* 1C3
* 1C4
* 7A2
* Aldose reductase
* Beta-Ketoacyl ACP reductase
* Carbohydrate dehydrogenases
* Carnitine dehydrogenase
* D-malate dehydrogenase (decarboxylating)
* DXP reductoisomerase
* Glucose-6-phosphate dehydrogenase
* Glycerol-3-phosphate dehydrogenase
* HMG-CoA reductase
* IMP dehydrogenase
* Isocitrate dehydrogenase
* Lactate dehydrogenase
* L-threonine dehydrogenase
* L-xylulose reductase
* Malate dehydrogenase
* Malate dehydrogenase (decarboxylating)
* Malate dehydrogenase (NADP+)
* Malate dehydrogenase (oxaloacetate-decarboxylating)
* Malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+)
* Phosphogluconate dehydrogenase
* Sorbitol dehydrogenase

* Hydroxysteroid dehydrogenase: 3β
* 3β-HSD
* NSDHL
* 11β
* 11β-HSD1
* 11β-HSD2
* 17β

1.1.2: cytochrome acceptor|

* D-lactate dehydrogenase (cytochrome)
* D-lactate dehydrogenase (cytochrome c-553)
* Mannitol dehydrogenase (cytochrome)

1.1.3: oxygen acceptor|

* Glucose oxidase
* L-gulonolactone oxidase
* Xanthine oxidase
* Alcohol oxidase

1.1.4: disulfide as acceptor|

* Vitamin K epoxide reductase
* Vitamin-K-epoxide reductase (warfarin-insensitive)

1.1.5: quinone/similar acceptor|

* Malate dehydrogenase (quinone)
* Quinoprotein glucose dehydrogenase

1.1.99: other acceptors|

* Choline dehydrogenase
* L2HGDH

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Enzymes

Activity|

* Active site
* Binding site
* Catalytic triad
* Oxyanion hole
* Enzyme promiscuity
* Diffusion-limited enzyme
* Cofactor
* Enzyme catalysis

Regulation|

* Allosteric regulation
* Cooperativity
* Enzyme inhibitor
* Enzyme activator

Classification|

* EC number
* Enzyme superfamily
* Enzyme family
* List of enzymes

Kinetics|

* Enzyme kinetics
* Eadie–Hofstee diagram
* Hanes–Woolf plot
* Lineweaver–Burk plot
* Michaelis–Menten kinetics

Types|

* EC1 Oxidoreductases (list)
* EC2 Transferases (list)
* EC3 Hydrolases (list)
* EC4 Lyases (list)
* EC5 Isomerases (list)
* EC6 Ligases (list)
* EC7 Translocases (list)

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