Glycoside hydrolase family 25

Protein family

Glycosyl hydrolases family 25

crystal structure of the bacterial lysozyme from streptomyces coelicolor at 1.65 a resolution

Identifiers
Symbol| Glyco_hydro_25
Pfam| PF01183
Pfam clan| CL0058
InterPro| IPR002053
PROSITE| PDOC00737
SCOP2| 1jfx / SCOPe / SUPFAM
CAZy| GH25
| Available protein structures:

Pfam  | structures / ECOD
PDB| RCSB PDB; PDBe; PDBj
PDBsum| structure summary

In molecular biology, glycoside hydrolase family 25 is a family of glycoside hydrolases.

Glycoside hydrolases EC 3.2.1. are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycoside hydrolases, based on sequence similarity, has led to the definition of >100 different families.[1][2][3] This classification is available on the CAZy web site,[4][5] and also discussed at CAZypedia, an online encyclopedia of carbohydrate active enzymes.[6][7]

Glycoside hydrolase family 25 CAZY GH_25 comprises enzymes with only one known activity; lysozyme (EC 3.2.1.17). It has been shown[8][9] that a number of cell-wall lytic enzymes are evolutionary related and can be classified into a single family. Two residues, an aspartate and a glutamate, have been shown[10] to be important for the catalytic activity of the Charalopsis enzyme. These residues as well as some others in their vicinity are conserved in all proteins from this family.

## References

[edit]

1. ^ Henrissat B, Callebaut I, Fabrega S, Lehn P, Mornon JP, Davies G (July 1995). "Conserved catalytic machinery and the prediction of a common fold for several families of glycosyl hydrolases". Proceedings of the National Academy of Sciences of the United States of America. 92 (15): 7090–4\. Bibcode:1995PNAS...92.7090H. doi:10.1073/pnas.92.15.7090. PMC 41477. PMID 7624375.
2. ^ Davies G, Henrissat B (September 1995). "Structures and mechanisms of glycosyl hydrolases". Structure. 3 (9): 853–9\. doi:10.1016/S0969-2126(01)00220-9. PMID 8535779.
3. ^ Henrissat B, Bairoch A (June 1996). "Updating the sequence-based classification of glycosyl hydrolases". The Biochemical Journal. 316 (Pt 2): 695–6\. doi:10.1042/bj3160695. PMC 1217404. PMID 8687420.
4. ^ "Home". CAZy.org. Retrieved 2018-03-06.
5. ^ Lombard V, Golaconda Ramulu H, Drula E, Coutinho PM, Henrissat B (January 2014). "The carbohydrate-active enzymes database (CAZy) in 2013". Nucleic Acids Research. 42 (Database issue): D490-5. doi:10.1093/nar/gkt1178. PMC 3965031. PMID 24270786.
6. ^ "Glycoside Hydrolase Family 25". CAZypedia.org. Retrieved 2018-03-06.
7. ^ CAZypedia Consortium (December 2018). "Ten years of CAZypedia: a living encyclopedia of carbohydrate-active enzymes" (PDF). Glycobiology. 28 (1): 3–8\. doi:10.1093/glycob/cwx089. PMID 29040563.
8. ^ Henrissat B (December 1991). "A classification of glycosyl hydrolases based on amino acid sequence similarities". The Biochemical Journal. 280 (2): 309–16\. doi:10.1042/bj2800309. PMC 1130547. PMID 1747104.
9. ^ Croux C, García JL (July 1991). "Sequence of the lyc gene encoding the autolytic lysozyme of Clostridium acetobutylicum ATCC824: comparison with other lytic enzymes". Gene. 104 (1): 25–31\. doi:10.1016/0378-1119(91)90460-S. PMID 1916274.
10. ^ Fouche PB, Hash JH (October 1978). "The N,O-diacetylmuramidase of Chalaropsis species. Identification of aspartyl and glutamyl residues in the active site". The Journal of Biological Chemistry. 253 (19): 6787–93\. doi:10.1016/S0021-9258(17)37988-7. PMID 567645.

This article incorporates text from the public domain Pfam and InterPro: IPR002053

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Hydrolase: sugar hydrolases (EC 3.2)

3.2.1: Glycoside hydrolases|

| Disaccharidase|

* Sucrase/Sucrase-isomaltase/Invertase
* Maltase
* Trehalase
* Lactase

|

Glucosidases|

* Cellulase
* Alpha-glucosidase
* Acid
* Neutral AB
* Neutral C
* Beta-glucosidase
* cytosolic
* Debranching enzyme

Other|

* Amylase
* Alpha
* Beta
* Chitinase
* Lysozyme
* Neuraminidase
* NEU1
* NEU2
* NEU3
* NEU4
* Bacterial neuraminidase
* Viral neuraminidase
* Galactosidases
* Alpha
* Beta
* alpha-Mannosidase
* Glucuronidase
* Klotho
* Hyaluronidase
* Pullulanase
* Glucosylceramidase
* lysosomal
* non-lysosomal
* Galactosylceramidase
* Alpha-N-acetylgalactosaminidase
* NAGA
* Alpha-N-acetylglucosaminidase
* Fucosidase
* Hexosaminidase
* HEXA
* HEXB
* Iduronidase
* Maltase-glucoamylase
* Heparanase
* HPSE2

3.2.2: Hydrolysing
N-Glycosyl compounds|

* DNA glycosylases: Oxoguanine glycosylase

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Enzymes

Activity|

* Active site
* Binding site
* Catalytic triad
* Oxyanion hole
* Enzyme promiscuity
* Diffusion-limited enzyme
* Cofactor
* Enzyme catalysis

Regulation|

* Allosteric regulation
* Cooperativity
* Enzyme inhibitor
* Enzyme activator

Classification|

* EC number
* Enzyme superfamily
* Enzyme family
* List of enzymes

Kinetics|

* Enzyme kinetics
* Eadie–Hofstee diagram
* Hanes–Woolf plot
* Lineweaver–Burk plot
* Michaelis–Menten kinetics

Types|

* EC1 Oxidoreductases (list)
* EC2 Transferases (list)
* EC3 Hydrolases (list)
* EC4 Lyases (list)
* EC5 Isomerases (list)
* EC6 Ligases (list)
* EC7 Translocases (list)

Portal:

* Biology

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