List of restriction enzyme cutting sites: T–Z

Main articles: List of restriction enzyme cutting sites and Restriction enzyme

Legend of nucleobases

Code  | Nucleotide represented
A  | Adenine (A)
C  | Cytosine (C)
G  | Guanine (G)
T  | Thymine (T)
N  | A, C, G or T
M  | A or C
R  | A or G
W  | A or T
Y  | C or T
S  | C or G
K  | G or T
H  | A, C or T
B  | C, G or T
V  | A, C or G
D  | A, G or T

This article contains a list of the most studied restriction enzymes whose names start with T to Z inclusive. It contains approximately 70 enzymes.

The following information is given:

* Enzyme: Accepted name of the molecule, according to the internationally adopted nomenclature[1][2], and bibliographical references. (Further reading: see the section "Nomenclature" in the article "Restriction enzyme".)
* PDB code: Code used to identify the structure of a protein in the PDB database of protein structures. The 3D atomic structure of a protein provides highly valuable information to understand the intimate details of its mechanism of action[3][4].
* Source: Organism that naturally produces the enzyme.
* Recognition sequence: Sequence of DNA recognized by the enzyme and to which it specifically binds.
* Cut: Cutting site and DNA products of the cut. The recognition sequence and the cutting site usually match, but sometimes the cutting site can be dozens of nucleotides away from the recognition site[5][6].
* Isoschizomers and neoschizomers: An isoschizomer is an enzyme that recognizes the same sequence as another. A neoschizomer is a special type of isoschizomer that recognizes the same sequence as another, but cuts in a different manner. A maximum number of 8-10 most common isoschizomers are indicated for every enzyme but there may be many more. Neoschizomers are shown in bold and green color font (e.g.: BamHI). When "None on date" is indicated, that means that there were no registered isoschizomers in the databases on that date with a clearly defined cutting site. Isoschizomers indicated in white font and grey background correspond to enzymes not listed in the current lists:

as in this not listed enzyme:  |  EcoR70I

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## Whole list navigation[edit]

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* – External links
* See also

## Restriction enzymes[edit]

### T[edit]

Current list/Article:

* Whole list navigation
* T
* U
* V
* X
* Y
* Z

Enzyme | PDB code | Source | Recognition sequence | Cut | Isoschizomers

|
|
|
|
|

TaaI |  | Thermus aquaticus Vn 4-311 | ` 5' ACNGT `
` 3' TGNCA ` | ` 5' 
ACN  GT
 3' `
` 3' 
TG  NCA
 5' ` |
TaiI |  | Thermus aquaticus cs1-331 | ` 5' ACGT `
` 3' TGCA ` | ` 5' 
ACGT  
 3' `
` 3' 
  TGCA
 5' ` |
TaqI |  | Thermus aquaticus | ` 5' TCGA `
` 3' AGCT ` | ` 5' 
T  CGA
 3' `
` 3' 
AGC  T
 5' ` |
TaqII |  | Thermus aquaticus YTI | ` 5' GACCGA `
` 3' CTGGCT ` | ` 5' 
GACCGAN8NNN  
 3' `
` 3' 
CTGGCTN8N  NN
 5' ` |
Taq52I |  | Thermus aquaticus YS52 | ` 5' GCWGC `
` 3' CGWCG ` | ` 5' 
G  CWGC
 3' `
` 3' 
CGWC  G
 5' ` | AceI, ApeKI, SuiI, TseI
TaqXI |  | Thermus aquaticus | ` 5' CCWGG `
` 3' GGWCC ` | ` 5' 
CC  WGG
 3' `
` 3' 
GGW  CC
 5' ` | ApaORI, BseBI, BsiLI, BstNI, BstOI, Bst2UI, MvaI, SleI, SspAI
TasI |  | Thermus aquaticus Vn 4-211 | ` 5' AATT `
` 3' TTAA ` | ` 5' 
  AATT
 3' `
` 3' 
TTAA  
 5' ` |
TatI |  | Thermus aquaticus CBA1-331 | ` 5' WGTACW `
` 3' WCATGW ` | ` 5' 
W  GTACW
 3' `
` 3' 
WCATG  W
 5' ` |
TauI |  | Thermus aquaticus | ` 5' GCSGC `
` 3' CGSCG ` | ` 5' 
GCSG  C
 3' `
` 3' 
C  GSCG
 5' ` |
TelI |  | Tolypothrix elabens | ` 5' GACNNNGTC `
` 3' CTGNNNCAG ` | ` 5' 
GACN  NNGTC
 3' `
` 3' 
CTGNN  NCAG
 5' ` | AspI, AtsI, PflFI, PsyI, Tth111I
TfiI |  | Thermus filiformis | ` 5' GAWTC `
` 3' CTWAG ` | ` 5' 
G  AWTC
 3' `
` 3' 
CTWA  G
 5' ` |
ThaI |  | Thermoplasma acidophilum | ` 5' CGCG `
` 3' GCGC ` | ` 5' 
CG  CG
 3' `
` 3' 
GC  GC
 5' ` | Bpu95I, Bsh1236I, Bsp50I, BstFNI, BstUI, Csp68KVI, FalII, FnuDII
TliI |  | Thermococcus litoralis | ` 5' CTCGAG `
` 3' GAGCTC ` | ` 5' 
C  TCGAG
 3' `
` 3' 
GAGCT  C
 5' ` | AbrI, BluI, BssHI, MavI, Sau3239I, Sfr274I, StrI, XhoI
Tru1I |  | Thermus ruber RFL1 | ` 5' TTAA `
` 3' AATT ` | ` 5' 
T  TAA
 3' `
` 3' 
AAT  T
 5' ` |
Tru9I |  | Thermus ruber 9 | ` 5' TTAA `
` 3' AATT ` | ` 5' 
T  TAA
 3' `
` 3' 
AAT  T
 5' ` |
Tru201I |  | Thermus ruber 201 | ` 5' RGATCY `
` 3' YCTAGR ` | ` 5' 
R  GATCY
 3' `
` 3' 
YCTAG  R
 5' ` |
TscI |  | Thermus sp. 491A | ` 5' ACGT `
` 3' TGCA ` | ` 5' 
ACGT  
 3' `
` 3' 
  TGCA
 5' ` |
TseI |  | Thermus sp. 93170 | ` 5' GCWGC `
` 3' CGWCG ` | ` 5' 
G  CWGC
 3' `
` 3' 
CGWC  G
 5' ` | AceI, ApeKI, SuiI, Taq52I
Tsp1I |  | Thermus sp. 1 | ` 5' ACTGG `
` 3' TGACC ` | ` 5' 
ACTGGN  
 3' `
` 3' 
TGAC  CN
 5' ` |
Tsp32I |  | Thermus sp. 32 | ` 5' TCGA `
` 3' AGCT ` | ` 5' 
T  CGA
 3' `
` 3' 
AGC  T
 5' ` |
Tsp32II |  | Thermus sp. 32 | ` 5' TCGA `
` 3' AGCT ` | ` 5' 
T  CGA
 3' `
` 3' 
AGC  T
 5' ` |
Tsp45I |  | Thermus sp. YS45 | ` 5' GTSAC `
` 3' CASTG ` | ` 5' 
  GTSAC
 3' `
` 3' 
CASTG  
 5' ` |
Tsp49I |  | Thermus sp. | ` 5' ACGT `
` 3' TGCA ` | ` 5' 
ACGT  
 3' `
` 3' 
  TGCA
 5' ` |
Tsp509I |  | Thermus sp. | ` 5' AATT `
` 3' TTAA ` | ` 5' 
  AATT
 3' `
` 3' 
TTAA  
 5' ` |
TspBI |  | Thermophilic sp. | ` 5' CCRYGG `
` 3' GGYRCC ` | ` 5' 
C  CRYGG
 3' `
` 3' 
GGYRC  C
 5' ` |
Tsp4CI |  | Thermus sp. 4C | ` 5' ACNGT `
` 3' TGNCA ` | ` 5' 
ACN  GT
 3' `
` 3' 
TG  NCA
 5' ` |
TspDTI |  | Thermus sp. DT | ` 5' ATGAA `
` 3' TACTT ` | ` 5' 
ATGAAN8NNN  
 3' `
` 3' 
TACTTN8N  NN
 5' ` |  — None on May 2010 —
TspEI |  | Thermus sp. 1E | ` 5' AATT `
` 3' TTAA ` | ` 5' 
  AATT
 3' `
` 3' 
TTAA  
 5' ` |
Tsp8EI |  | Thermus sp. 8E | ` 5' GCCN5GGC `
` 3' CGGN5CCG ` | ` 5' 
GCCNNNN  NGGC
 3' `
` 3' 
CGGN  NNNNCCG
 5' ` |
TspGWI |  | Thermus sp. GW | ` 5' ACGGA `
` 3' TGCCT ` | ` 5' 
ACGGAN8NNN  
 3' `
` 3' 
TGCCTN8N  NN
 5' ` |
TspMI = UthSI  |  | Unidentified thermophile  | ` 5' CCCGGG `
` 3' GGGCCC ` | ` 5' 
C  CCGGG
 3' `
` 3' 
GGGCC  C
 5' ` | AhyI, Cfr9I, EaeAI, EclRI, PaeBI, PspAI, SmaI, XcyI, XmaI, XmaCI
TspRI |  | Thermus sp. R | ` 5' CASTGNN `
` 3' GTSAC ` | ` 5' 
CASTGNN  
 3' `
` 3' 
  GTSACNN
 5' ` |
Tth111I |  | Thermus thermophilus 111 | ` 5' GACNNNGTC `
` 3' CTGNNNCAG ` | ` 5' 
GACN  NNGTC
 3' `
` 3' 
CTGNN  NCAG
 5' ` | AspI, AtsI, PflFI, PsyI, TelI
Tth111II |  | Thermus thermophilus 111 | ` 5' CAARCA `
` 3' GTTYGT ` | ` 5' 
CAARCAN8NNN  
 3' `
` 3' 
GTTYGTN8N  NN
 5' ` |
TthHB8I |  | Thermus thermophilus HB8 | ` 5' TCGA `
` 3' AGCT ` | ` 5' 
T  CGA
 3' `
` 3' 
AGC  T
 5' ` |

This list is incomplete; you can help by adding missing items. (February 2011)

|

### U[edit]

Current list/Article:

* Top
* Whole list navigation
* T
* U
* V
* X
* Y
* Z

Enzyme | PDB code | Source | Recognition sequence | Cut | Isoschizomers

|
|
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|

Uba4009I |  | Unidentified bacterium A  | ` 5' GGATCC `
` 3' CCTAGG ` | ` 5' 
G  GATCC
 3' `
` 3' 
CCTAG  G
 5' ` | AccEBI, AliI, ApaCI, AsiI, BamHI, BnaI, BspAAIII, RspLKII, SolI
Uba153AI |  | Unidentified bacterium 153A  | ` 5' CAGCTG `
` 3' GTCGAC ` | ` 5' 
CAG  CTG
 3' `
` 3' 
GTC  GAC
 5' ` |
UbaM39I |  | Unidentified bacterium M39  | ` 5' CAGCTG `
` 3' GTCGAC ` | ` 5' 
CAG  CTG
 3' `
` 3' 
GTC  GAC
 5' ` |
UnbI |  | Unidentified bacterium #8  | ` 5' GGNCC `
` 3' CCNGG ` | ` 5' 
  GGNCC
 3' `
` 3' 
CCNGG  
 5' ` | AvcI, BavAII, Bce22I, Bsp1894I, Bsu54I, FmuI, NspIV, UnbI
Uur960I |  | Ureaplasma urealyticum 960 | ` 5' GCNGC `
` 3' CGNCG ` | ` 5' 
GC  NGC
 3' `
` 3' 
CGN  CG
 5' ` |

This list is incomplete; you can help by adding missing items. (February 2011)

|

### V[edit]

Current list/Article:

* Top
* Whole list navigation
* T
* U
* V
* X
* Y
* Z

Enzyme | PDB code | Source | Recognition sequence | Cut | Isoschizomers

|
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Van91I |  | Vibrio anguillarum RFL91 | ` 5' CCAN5TGG `
` 3' GGTN5ACC ` | ` 5' 
CCANNNN  NTGG
 3' `
` 3' 
GGTN  NNNNACC
 5' ` | AccB7I, AcpII, Asp10HII, BasI, Esp1396I, PflBI, PflMI
Vha464I |  | Vibrio harveyi 464 | ` 5' CTTAAG `
` 3' GAATTC ` | ` 5' 
C  TTAAG
 3' `
` 3' 
GAATT  C
 5' ` |
VneI |  | Vibrio nereis 18 | ` 5' GTGCAC `
` 3' CACGTG ` | ` 5' 
G  TGCAC
 3' `
` 3' 
CACGT  G
 5' ` | Alw44I, ApaLI, SnoI
VpaK32I |  | Vibrio parahaemolyticus 4387-61 | ` 5' GCTCTTC `
` 3' CGAGAAG ` | ` 5' 
GCTCTTCN  NNN
 3' `
` 3' 
CGAGAAGNNNN  
 5' ` |
VpaK11AI |  | Vibrio parahaemolyticus 1743 | ` 5' GGWCC `
` 3' CCWGG ` | ` 5' 
  GGWCC
 3' `
` 3' 
CCWGG  
 5' ` | Bme216I, CauI, EagMI, FdiI, HgiBI, HgiHIII, SinI, VpaK11BI
VpaK11BI |  | Vibrio parahaemolyticus 1743-1 | ` 5' GGWCC `
` 3' CCWGG ` | ` 5' 
G  GWCC
 3' `
` 3' 
CCWG  G
 5' ` | BsrAI, CauI, EagMI, FdiI, HgiBI, HgiJI, SinI, VpaK11AI
VspI |  | Vibrio sp. 343 | ` 5' ATTAAT `
` 3' TAATTA ` | ` 5' 
AT  TAAT
 3' `
` 3' 
TAAT  TA
 5' ` | AseI, AsnI, BpoAI, PshBI, Sru4DI

This list is incomplete; you can help by adding missing items. (February 2011)

|

### X[edit]

Current list/Article:

* Top
* Whole list navigation
* T
* U
* V
* X
* Y
* Z

Enzyme | PDB code | Source | Recognition sequence | Cut | Isoschizomers

|
|
|
|
|

XagI |  | Xanthobacter agilis Vs 18-132 | ` 5' CCTN5AGG `
` 3' GGAN5TCC ` | ` 5' 
CCTNN  NNNAGG
 3' `
` 3' 
GGANNN  NNTCC
 5' ` |
XapI |  | Xanthomonas ampelina Slo 51-021 | ` 5' RAATTY `
` 3' YTTAAR ` | ` 5' 
R  AATTY
 3' `
` 3' 
YTTAA  R
 5' ` |  | AcsI, ApoI,  |  CfaI, | FsiI

|
|

XbaI[7] |  | Xanthomonas badrii | ` 5' TCTAGA `
` 3' AGATCT ` | ` 5' 
T  CTAGA
 3' `
` 3' 
AGATC  T
 5' ` |
XcaI |  | Xanthomonas campestris | ` 5' GTATAC `
` 3' CATATG ` | ` 5' 
GTA  TAC
 3' `
` 3' 
CAT  ATG
 5' ` |
XceI |  | Xanthomonas campestris Ast 40-024 | ` 5' RCATGY `
` 3' YGTACR ` | ` 5' 
RCATG  Y
 3' `
` 3' 
Y  GTACR
 5' ` |
XciI |  | Xanthomonas citri | ` 5' GTCGAC `
` 3' CAGCTG ` | ` 5' 
G  TCGAC
 3' `
` 3' 
CAGCT  G
 5' ` |
XcmI |  | Xanthomonas campestris | ` 5' CCAN9TGG `
` 3' GGTN9ACC ` | ` 5' 
CCANNNNN  NNNNTGG
 3' `
` 3' 
GGTNNNN  NNNNNACC
 5' ` |
XcyI |  | Xanthomonas cyanopsidis 13D5 | ` 5' CCCGGG `
` 3' GGGCCC ` | ` 5' 
C  CCGGG
 3' `
` 3' 
GGGCC  C
 5' ` | CfrJ4I, EaeAI, EclRI, Pac25I, PspAI, TspMI, XmaI, XmaCI
XhoI |  | Xanthomonas holcicola | ` 5' CTCGAG `
` 3' GAGCTC ` | ` 5' 
C  TCGAG
 3' `
` 3' 
GAGCT  C
 5' ` | AbrI, BluI, BssHI, PanI, Sau3239I, Sfr274I, TliI, XpaI
XhoII |  | Xanthomonas holcicola | ` 5' RGATCY `
` 3' YCTAGR ` | ` 5' 
R  GATCY
 3' `
` 3' 
YCTAG  R
 5' ` |
XmaI |  | Xanthomonas malvacearum | ` 5' CCCGGG `
` 3' GGGCCC ` | ` 5' 
C  CCGGG
 3' `
` 3' 
GGGCC  C
 5' ` | AhyI, Cfr9I, EaeAI, EclRI, PaeBI, PspAI, TspMI, XcyI, XmaCI
XmaIII |  | Xanthomonas malvacearum | ` 5' CGGCCG `
` 3' GCCGGC ` | ` 5' 
C  GGCCG
 3' `
` 3' 
GCCGG  C
 5' ` | AaaI, BseX3I, BstZI, EagI, EclXI, Eco52I, SenPT16I
XmaCI |  | Xanthomonas malvacearum C | ` 5' CCCGGG `
` 3' GGGCCC ` | ` 5' 
C  CCGGG
 3' `
` 3' 
GGGCC  C
 5' ` | AhyI, Cfr9I, EaeAI, EclRI, PaeBI, Pac25I, PspAI, TspMI, XcyI, XmaI
XmaJI |  | Xanthomonas maltophilia Jo 85-025 | ` 5' CCTAGG `
` 3' GGATCC ` | ` 5' 
C  CTAGG
 3' `
` 3' 
GGATC  C
 5' ` | AspA2I, AvrII, AvrBII, BlnI, BspA2I
XmiI |  | Xanthomonas maltophilia Jo 21-021 | ` 5' GTMKAC `
` 3' CAKMTG ` | ` 5' 
GT  MKAC
 3' `
` 3' 
CAKM  TG
 5' ` | AccI, FblI
XmnI |  | Xanthomonas manihotis 7AS1 | ` 5' GAAN4TTC `
` 3' CTTN4AAG ` | ` 5' 
GAANN  NNTTC
 3' `
` 3' 
CTTNN  NNAAG
 5' ` | Asp700I, BbvAI, MroXI, PdmI
XorII |  | Xanthomonas oryzae | ` 5' CGATCG `
` 3' GCTAGC ` | ` 5' 
CGAT  CG
 3' `
` 3' 
GC  TAGC
 5' ` | Afa16RI, BspCI, EagBI, ErhB9I, MvrI, Ple19I, PvuI, RshI
XpaI |  | Xanthomonas papavericola | ` 5' CTCGAG `
` 3' GAGCTC ` | ` 5' 
C  TCGAG
 3' `
` 3' 
GAGCT  C
 5' ` | BssHI, MavI, PanI, SauLPII, Sbi68I, Sol10179I, StrI, XhoI
XspI |  | Xanthomonas sp. YK1 | ` 5' CTAG `
` 3' GATC ` | ` 5' 
C  TAG
 3' `
` 3' 
GAT  C
 5' ` |

This list is incomplete; you can help by adding missing items. (February 2011)

|

### Y[edit]

Current list/Article:

* Top
* Whole list navigation
* T
* U
* V
* X
* Y
* Z

Enzyme | PDB code | Source | Recognition sequence | Cut | Isoschizomers

|
|
|
|
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YenI |  | Yersinia enterocolitica 08 A2635 | ` 5' CTGCAG `
` 3' GACGTC ` | ` 5' 
CTGCA  G
 3' `
` 3' 
G  ACGTC
 5' ` | Asp713I, BsuBI, CfrA4I, Ecl37kI, Psp23I, PstI, SalPI, SflI, Sst12I

This list is incomplete; you can help by adding missing items. (February 2011)

|

### Z[edit]

Current list/Article:

* Top
* Whole list navigation
* T
* U
* V
* X
* Y
* Z

Enzyme | PDB code | Source | Recognition sequence | Cut | Isoschizomers

|
|
|
|
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ZanI |  | Zymomonas anaerobia | ` 5' CCWGG `
` 3' GGWCC ` | ` 5' 
CC  WGG
 3' `
` 3' 
GGW  CC
 5' ` | ApaORI, BseBI, BspNI, BstNI, Bst2UI, CthII, EcoRII, MvaI, SspAI
ZhoI |  | Zymomonas holcicola | ` 5' ATCGAT `
` 3' TAGCTA ` | ` 5' 
AT  CGAT
 3' `
` 3' 
TAGC  TA
 5' ` | BanIII, BbvAII, BscI, BspJI, ClaI, LcaI, PgaI, SpmI, Ssp27144I
ZraI |  | Zoogloea ramigera 11 | ` 5' GACGTC `
` 3' CTGCAG ` | ` 5' 
GAC  GTC
 3' `
` 3' 
CTG  CAG
 5' ` | AatII, Ssp5230I
ZrmI |  | Zoogloea ramigera SCA | ` 5' AGTACT `
` 3' TCATGA ` | ` 5' 
AGT  ACT
 3' `
` 3' 
TCA  TGA
 5' ` |  | Acc113I, AssI,  |  BmcAI, Bpa34I,

|

DpaI, Eco255I, RflFII, ScaI
Zsp2I |  | Zoogloea sp. 2 | ` 5' ATGCAT `
` 3' TACGTA ` | ` 5' 
ATGCA  T
 3' `
` 3' 
T  ACGTA
 5'` | BfrBI, Csp68KIII, EcoT22I, NsiI, PinBI, Ppu10I, SepI, SspD5II

This list is incomplete; you can help by adding missing items. (February 2011)

|

Current list/Article:

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## Notes[edit]

1. ^ Smith HO, Nathans D (December 1973). "Letter: A suggested nomenclature for bacterial host modification and restriction systems and their enzymes". J. Mol. Biol. 81 (3): 419–23. doi:10.1016/0022-2836(73)90152-6. PMID 4588280.
2. ^ Roberts RJ, Belfort M, Bestor T, Bhagwat AS, Bickle TA, Bitinaite J, Blumenthal RM, Degtyarev SK, Dryden DT, Dybvig K, Firman K, Gromova ES, Gumport RI, Halford SE, Hattman S, Heitman J, Hornby DP, Janulaitis A, Jeltsch A, Josephsen J, Kiss A, Klaenhammer TR, Kobayashi I, Kong H, Krüger DH, Lacks S, Marinus MG, Miyahara M, Morgan RD, Murray NE, Nagaraja V, Piekarowicz A, Pingoud A, Raleigh E, Rao DN, Reich N, Repin VE, Selker EU, Shaw PC, Stein DC, Stoddard BL, Szybalski W, Trautner TA, Van Etten JL, Vitor JM, Wilson GG, Xu SY (April 2003). "A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes". Nucleic Acids Res. 31 (7): 1805–12. doi:10.1093/nar/gkg274. PMC 152790. PMID 12654995.
3. ^ Jeremy MB, John LT, Lubert S (2002). "3. Protein Structure and Function". Biochemistry. San Francisco: W. H. Freeman. ISBN 0-7167-4684-0.
4. ^ Anfinsen C.B. (1973). "Principles that Govern the Folding of Protein Chains". Science. 181 (4096): 223–30. doi:10.1126/science.181.4096.223. PMID 4124164.
5. ^ Kessler C, Manta V (August 1990). "Specificity of restriction endonucleases and DNA modification methyltransferases a review (Edition 3)". Gene. 92 (1–2): 1–248. doi:10.1016/0378-1119(90)90486-B. PMID 2172084.
6. ^ Pingoud A, Jeltsch A (September 2001). "Structure and function of type II restriction endonucleases". Nucleic Acids Res. 29 (18): 3705–27. doi:10.1093/nar/29.18.3705. PMC 55916. PMID 11557805.
7. ^ Monty Krieger; Matthew P Scott; Matsudaira, Paul T.; Lodish, Harvey F.; Darnell, James E.; Lawrence Zipursky; Kaiser, Chris; Arnold Berk (2004). Molecular Cell Biology (5th ed.). New York: W.H. Freeman and Company. ISBN 0-7167-4366-3.

* v
* t
* e

Restriction modification system: restriction enzyme

Basic Concept|

* Isoschizomer
* Neoschizomer
* Isocaudomer

Recognition sequence 4bp|

* TaqI
* Sau3A
* AluI*
* HaeIII*
* DpnII
* NlaIII
* HpaII

Recognition sequence 5bp|

* EcoRII
* FokI

Recognition sequence 6bp|

* AaaI
* BglII
* PovII*
* EcoRV*
* EcoRI
* BamHI
* HindIII
* XbaI
* XhoI
* SacI
* PstI
* NdeI

Recognition sequence 8bp|

* NotI

Lists|

* A
* Ba–Bc
* Bd–Bp
* Bsa–Bso
* Bsp–Bss
* Bst–Bv
* C-D
* E-F
* G-K
* L-N
* O-R
* S
* T-Z

* means cleavage produces blunt ends
*[v]: View this template
*[t]: Discuss this template
*[e]: Edit this template