List of restriction enzyme cutting sites: S

Main articles: List of restriction enzyme cutting sites and Restriction enzyme

Legend of nucleobases

Code  | Nucleotide represented
A  | Adenine (A)
C  | Cytosine (C)
G  | Guanine (G)
T  | Thymine (T)
N  | A, C, G or T
M  | A or C
R  | A or G
W  | A or T
Y  | C or T
S  | C or G
K  | G or T
H  | A, C or T
B  | C, G or T
V  | A, C or G
D  | A, G or T

This article contains a list of the most studied restriction enzymes whose names start with S. It contains approximately 130 enzymes. The following information is given:

* Enzyme: Accepted name of the molecule, according to the internationally adopted nomenclature[1][2], and bibliographical references. (Further reading: see the section "Nomenclature" in the article "Restriction enzyme".)
* PDB code: Code used to identify the structure of a protein in the PDB database of protein structures. The 3D atomic structure of a protein provides highly valuable information to understand the intimate details of its mechanism of action[3][4].
* Source: Organism that naturally produces the enzyme.
* Recognition sequence: Sequence of DNA recognized by the enzyme and to which it specifically binds.
* Cut: Cutting site and DNA products of the cut. The recognition sequence and the cutting site usually match, but sometimes the cutting site can be dozens of nucleotides away from the recognition site[5][6].
* Isoschizomers and neoschizomers: An isoschizomer is an enzyme that recognizes the same sequence as another. A neoschizomer is a special type of isoschizomer that recognizes the same sequence as another, but cuts in a different manner. A maximum number of 8-10 most common isoschizomers are indicated for every enzyme but there may be many more. Neoschizomers are shown in bold and green color font (e.g.: BamHI). When "None on date" is indicated, that means that there were no registered isoschizomers in the databases on that date with a clearly defined cutting site. Isoschizomers indicated in white font and grey background correspond to enzymes not listed in the current lists:

as in this not listed enzyme:  |  EcoR70I

|

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## Restriction enzymes

[edit]

### S

[edit]

Enzyme | PDB code | Source | Recognition sequence | Cut | Isoschizomers

|
|
|
|
|

SacI[7] |  | Streptomyces achromogenes | ` 5' GAGCTC `
` 3' CTCGAG ` | ` 5' 
GAGCT  C
 3' `
` 3' 
C  TCGAG
 5' ` |
SacII |  | Streptomyces achromogenes | ` 5' CCGCGG `
` 3' GGCGCC ` | ` 5' 
CCGC  GG
 3' `
` 3' 
GG  CGCC
 5' ` | Cfr42I
SacNI |  | Streptomyces achromogenes N-J-H | ` 5' GRGCYC `
` 3' CYCGRG ` | ` 5' 
GRGCY  C
 3' `
` 3' 
C  YCGRG
 5' ` |
SalI[7] |  | Streptomyces albus | ` 5' GTCGAC `
` 3' CAGCTG ` | ` 5' 
G  TCGAC
 3' `
` 3' 
CAGCT  G
 5' ` |
SalPI |  | Streptomyces albus | ` 5' CTGCAG `
` 3' GACGTC ` | ` 5' 
CTGCA  G
 3' `
` 3' 
G  ACGTC
 5' ` | ApiI, BspMAI, CstI, Ecl37kI, Ecl2zI, Psp23I, PstI, Srl5DI, YenI
SanDI |  | Streptomyces sp. | ` 5' GGGWCCC `
` 3' CCCWGGG ` | ` 5' 
GG  GWCCC
 3' `
` 3' 
CCCWG  GG
 5' ` |
SapI |  | Saccharopolyspora sp. | ` 5' GCTCTTC `
` 3' CGAGAAG ` | ` 5' 
GCTCTTCN  NNN
 3' `
` 3' 
CGAGAAGNNNN  
 5' ` |
SarI |  | Streptomyces aureomonopodiales | ` 5' AGGCCT `
` 3' TCCGGA ` | ` 5' 
AGG  CCT
 3' `
` 3' 
TCC  GGA
 5' ` | AatI, AspMI, Eco147I, GdiI, PceI, Pme55I, Sru30DI, SseBI, SteI, StuI
SatI |  | Staphylococcus arlettae RFL832 | ` 5' GCNGC `
` 3' CGNCG ` | ` 5' 
GC  NGC
 3' `
` 3' 
CGN  CG
 5' ` |
SauI |  | Streptomyces aureofaciens IKA 18/4 | ` 5' CCTNAGG `
` 3' GGANTCC ` | ` 5' 
CC  TNAGG
 3' `
` 3' 
GGANT  CC
 5' ` | AocI, BliHKI, BspR7I, CvnI, Lmu60I, OxaNI, SauI, SshAI
Sau96I |  | Staphylococcus aureus PS96 | ` 5' GGNCC `
` 3' CCNGG ` | ` 5' 
G  GNCC
 3' `
` 3' 
CCNG  G
 5' ` | AsuI, Bal228I, Bce22I, BsiZI, BspF4I, Cfr13I, Nsp7121I, UnbI
Sau3239I |  | Streptomyces aureofaciens | ` 5' CTCGAG `
` 3' GAGCTC ` | ` 5' 
C  TCGAG
 3' `
` 3' 
GAGCT  C
 5' ` | BspAAI, BstVI, MlaAI, PaeR7I, Sbi68I, Sfr274I, SlaI, XpaI
Sau3AI | 2REU | Staphylococcus aureus 3A | ` 5' GATC `
` 3' CTAG ` | ` 5' 
  GATC
 3' `
` 3' 
CTAG  
 5' ` | Bce243I, Bsp143I, BspJI, BstMBI, CviAI, Kzo9I, NdeII, Sth368I
SauBMKI |  | Streptomyces aureofaciens BM-K | ` 5' GCCGGC `
` 3' CGGCCG ` | ` 5' 
GCC  GGC
 3' `
` 3' 
CGG  CCG
 5' ` |
SauHPI |  | Streptomyces aureofaciens R8/26 | ` 5' GCCGGC `
` 3' CGGCCG ` | ` 5' 
GCC  GGC
 3' `
` 3' 
CGG  CCG
 5' ` |
SauLPI |  | Streptomyces aureofaciens B-96 | ` 5' GCCGGC `
` 3' CGGCCG ` | ` 5' 
GCC  GGC
 3' `
` 3' 
CGG  CCG
 5' ` |
SauLPII |  | Streptomyces aureofaciens B-96 | ` 5' CTCGAG `
` 3' GAGCTC ` | ` 5' 
C  TCGAG
 3' `
` 3' 
GAGCT  C
 5' ` | BspAAI, MavI, MlaAI, PaeR7I, Sbi68I, Sfr274I, SlaI, XpaI
SauMI |  | Staphylococcus aureus M | ` 5' GATC `
` 3' CTAG ` | ` 5' 
  GATC
 3' `
` 3' 
CTAG  
 5' ` | BscFI, BspAI, BstKTI, ChaI, HacI, MkrAI, RalF40I, SsiAI
SauNI |  | Streptomyces aureofaciens NMU | ` 5' GCCGGC `
` 3' CGGCCG ` | ` 5' 
GCC  GGC
 3' `
` 3' 
CGG  CCG
 5' ` |
SauSI |  | Streptomyces aureofaciens 16 | ` 5' GCCGGC `
` 3' CGGCCG ` | ` 5' 
GCC  GGC
 3' `
` 3' 
CGG  CCG
 5' ` |
SbfI |  | Streptomyces sp. BF-61 | ` 5' CCTGCAGG `
` 3' GGACGTCC ` | ` 5' 
CCTGCA  GG
 3' `
` 3' 
GG  ACGTCC
 5' ` |
Sbi68I |  | Streptomyces bikiniensis JAM68 | ` 5' CTCGAG `
` 3' GAGCTC ` | ` 5' 
C  TCGAG
 3' `
` 3' 
GAGCT  C
 5' ` | BspAAI, MavI, MlaAI, PaeR7I, Sbi68I, Sol10179I, StrI, XpaI
Sbo13I |  | Shigella boydii 13 | ` 5' TCGCGA `
` 3' AGCGCT ` | ` 5' 
TCG  CGA
 3' `
` 3' 
AGC  GCT
 5' ` |
SbvI |  | Streptococcus bovis II/1 | ` 5' GGCC `
` 3' CCGG ` | ` 5' 
GG  CC
 3' `
` 3' 
CC  GG
 5' ` |
ScaI[7] |  | Streptomyces caespitosus | ` 5' AGTACT `
` 3' TCATGA ` | ` 5' 
AGT  ACT
 3' `
` 3' 
TCA  TGA
 5' ` |  | Acc113I, AssI,  |  BmcAI, Bpa34I,

|

DpaI, Eco255I, RflFII, ZrmI
SceIII |  | Saccharomyces cerevisiae | ` 5' GCCGGC `
` 3' CGGCCG ` | ` 5' 
G  CCGGC
 3' `
` 3' 
CGGCC  G
 5' ` |
SchI |  | Staphylococcus cohnii Lki 19-320 | ` 5' GAGTC `
` 3' CTCAG ` | ` 5' 
GAGTCN4N  
 3' `
` 3' 
CTCAGN4N  
 5' ` |
SchZI |  | Streptomyces chusanensis ZS-2 | ` 5' CCGCGG `
` 3' GGCGCC ` | ` 5' 
CCGC  GG
 3' `
` 3' 
GG  CGCC
 5' ` |
SciI |  | Streptoverticillium cinnamoneum | ` 5' CTCGAG `
` 3' GAGCTC ` | ` 5' 
CTC  GAG
 3' `
` 3' 
GAG  CTC
 5' ` | BspAAI, BstVI, MlaAI, PaeR7I, SauLPII, SlaI, Sol10179I, XhoI
SciNI |  | Spiroplasma citri ASP2 | ` 5' GCGC `
` 3' CGCG ` | ` 5' 
G  CGC
 3' `
` 3' 
CGC  G
 5' ` | BspLAI, BstHHI, CfoI, FnuDIII, HhaI, Hin6I, HinP1I, HsoI, HspAI
ScrFI |  | Streptococcus cremoris F | ` 5' CCNGG `
` 3' GGNCC ` | ` 5' 
CC  NGG
 3' `
` 3' 
GGN  CC
 5' ` |
SdaI | 2IXS | Streptomyces diastaticus Ng7-324 | ` 5' CCTGCAGG `
` 3' GGACGTCC ` | ` 5' 
CCTGCA  GG
 3' `
` 3' 
GG  ACGTCC
 5' ` |
SdiI |  | Streptomyces diastaticus | ` 5' GGCCN5GGCC `
` 3' CCGGN5CCGG ` | ` 5' 
GGCCNNNN  NGGCC
 3' `
` 3' 
CCGGN  NNNNCCGG
 5' ` |
SduI |  | Streptococcus durans RFL3 | ` 5' GDGCHC `
` 3' CHCGDG ` | ` 5' 
GDGCH  C
 3' `
` 3' 
C  HCGDG
 5' ` | AocII, BmyI, BsoCI, Bsp1286I, BspLS2I, MhlI, NspII
SecI |  | Synechocystis sp. 6701 | ` 5' CCNNGG `
` 3' GGNNCC ` | ` 5' 
C  CNNGG
 3' `
` 3' 
GGNNC  C
 5' ` |
SelI |  | Synechococcus elongatus | ` 5' CGCG `
` 3' GCGC ` | ` 5' 
  CGCG
 3' `
` 3' 
GCGC  
 5' ` | AccII, Bsh1236I, Bsp123I, Bsp50I, BstFNI, BstUI, BtkI, Csp68KVI
SenPT16I |  | Salmonella enteritidis PT16 | ` 5' CGGCCG `
` 3' GCCGGC ` | ` 5' 
C  GGCCG
 3' `
` 3' 
GCCGG  C
 5' ` | AaaI, BseX3I, BstZI, EagI, EclXI, Eco52I, XmaIII
SenPT14bI |  | Salmonella enteritidis PT14b | ` 5' CCGCGG `
` 3' GGCGCC ` | ` 5' 
CCGC  GG
 3' `
` 3' 
GG  CGCC
 5' ` |
SepI |  | Staphylococcus epidermidis | ` 5' ATGCAT `
` 3' TACGTA ` | ` 5' 
ATGCA  T
 3' `
` 3' 
T  ACGTA
 5' ` | BfrBI, Csp68KIII, Mph1103I, NsiI, PinBI, Ppu10I, SspD5II, Zsp2I
SexAI |  | Streptomyces exfoliatus | ` 5' ACCWGGT `
` 3' TGGWCCA ` | ` 5' 
A  CCWGGT
 3' `
` 3' 
TGGWCC  A
 5' ` |
SexBI |  | Streptomyces exfoliatus | ` 5' CCGCGG `
` 3' GGCGCC ` | ` 5' 
CCGC  GG
 3' `
` 3' 
GG  CGCC
 5' ` |
SexCI |  | Streptomyces exfoliatus | ` 5' CCGCGG `
` 3' GGCGCC ` | ` 5' 
CCGC  GG
 3' `
` 3' 
GG  CGCC
 5' ` |
SfaI |  | Streptococcus faecalis zymogenes | ` 5' GGCC `
` 3' CCGG ` | ` 5' 
GG  CC
 3' `
` 3' 
CC  GG
 5' ` |
SfaNI |  | Streptococcus faecalis ND547 | ` 5' GCATC `
` 3' CGTAG ` | ` 5' 
GCATCN4N  NNNN
 3' `
` 3' 
CGTAGN4NNNNN  
 5' ` |
SfcI |  | Streptococcus faecium | ` 5' CTRYAG `
` 3' GAYRTC ` | ` 5' 
C  TRYAG
 3' `
` 3' 
GAYRT  C
 5' ` |
SfeI |  | Streptococcus faecalis | ` 5' CTRYAG `
` 3' GAYRTC ` | ` 5' 
C  TRYAG
 3' `
` 3' 
GAYRT  C
 5' ` |
SfiI | 2F03 | Streptomyces fimbriatus | ` 5' GGCCN5GGCC `
` 3' CCGGN5CCGG ` | ` 5' 
GGCCNNNN  NGGCC
 3' `
` 3' 
CCGGN  NNNNCCGG
 5' ` |
SflI |  | Streptoverticillium flavopersicum | ` 5' CTGCAG `
` 3' GACGTC ` | ` 5' 
CTGCA  G
 3' `
` 3' 
G  ACGTC
 5' ` | ApiI, BspMAI, BsuBI, CfrA4I, Psp23I, PstI, Sag23I, Srl5DI, YenI
SfoI |  | Serratia fonticola | ` 5' GGCGCC `
` 3' CCGCGG ` | ` 5' 
GGC  GCC
 3' `
` 3' 
CCG  CGG
 5' ` |
Sfr274I |  | Streptomyces fradiae 274 | ` 5' CTCGAG `
` 3' GAGCTC ` | ` 5' 
C  TCGAG
 3' `
` 3' 
GAGCT  C
 5' ` | AbrI, BluI, BssHI, MavI, Sau3239I, Sol10179I, StrI, TliI
Sfr303I |  | Streptomyces fradiae 303 | ` 5' CCGCGG `
` 3' GGCGCC ` | ` 5' 
CCGC  GG
 3' `
` 3' 
GG  CGCC
 5' ` |
SfuI |  | Streptomyces fulvissimus | ` 5' TTCGAA `
` 3' AAGCTT ` | ` 5' 
TT  CGAA
 3' `
` 3' 
AAGC  TT
 5' ` | AsuII, BimI, Bsp119I, CbiI, Csp68KII, PlaII, Ssp1I, SviI
SgfI |  | Streptomyces griseoruber | ` 5' GCGATCGC `
` 3' CGCTAGCG ` | ` 5' 
GCGAT  CGC
 3' `
` 3' 
CGC  TAGCG
 5' ` |  | AsiSI,  |  RgaI, SfaAI

|

SgrAI | 3DPG 3N7B 3N78 3MGY 3MG6 | Streptomyces griseus | ` 5' CRCCGGYG `
` 3' GYGGCCRC ` | ` 5' 
CR  CCGGYG
 3' `
` 3' 
GYGGCC  RC
 5' ` |
SgrBI |  | Streptomyces griseus | ` 5' CCGCGG `
` 3' GGCGCC ` | ` 5' 
CCGC  GG
 3' `
` 3' 
GG  CGCC
 5' ` |
SimI |  | Staphylococcus intermedius 6H | ` 5' GGGTC `
` 3' CCCAG ` | ` 5' 
GG  GTC
 3' `
` 3' 
CCCAG  
 5' ` |
SinI |  | Salmonella infantis | ` 5' GGWCC `
` 3' CCWGG ` | ` 5' 
G  GWCC
 3' `
` 3' 
CCWG  G
 5' ` | Bme216I, CauI, EagMI, FdiI, HgiBI, HgiHIII, Psp03I, VpaK11BI
SlaI |  | Streptomyces lavendulae | ` 5' CTCGAG `
` 3' GAGCTC ` | ` 5' 
C  TCGAG
 3' `
` 3' 
GAGCT  C
 5' ` | AbrI, BluI, BssHI, MavI, Sau3239I, Sfr274I, TliI, XhoI
SleI |  | Synechococcus leopoliensis | ` 5' CCWGG `
` 3' GGWCC ` | ` 5' 
  CCWGG
 3' `
` 3' 
GGWCC  
 5' ` | AeuI, AglI, ApaORI, BspNI, Bst2I, EcoRII, SniI, SslI, SspAI, ZanI
Slu1777I |  | Streptomyces lusitanus 1777 | ` 5' GCCGGC `
` 3' CGGCCG ` | ` 5' 
GCC  GGC
 3' `
` 3' 
CGG  CCG
 5' ` |
SmaI |  | Serratia marcescens Sb | ` 5' CCCGGG `
` 3' GGGCCC ` | ` 5' 
CCC  GGG
 3' `
` 3' 
GGG  CCC
 5' ` | AhyI, CfrJ4I, EaeAI, EclRI, Pac25I, PspAI, TspMI, XcyI, XmaI, XmaCI
SmiI |  | Streptococcus milleri S | ` 5' ATTTAAAT `
` 3' TAAATTTA ` | ` 5' 
ATTT  AAAT
 3' `
` 3' 
TAAA  TTTA
 5' ` |
SmiMI |  | Sphingobacterium mizutae M | ` 5' CAYN4RTG `
` 3' GTRN4YAC ` | ` 5' 
CAYNN  NNRTG
 3' `
` 3' 
GTRNN  NNYAC
 5' ` |
SmlI |  | Stenotrophomonas maltophilia | ` 5' CTYRAG `
` 3' GARYTC ` | ` 5' 
C  TYRAG
 3' `
` 3' 
GARYT  C
 5' ` |
SmuI |  | Sphingobacterium multivorum RFL21 | ` 5' CCCGC `
` 3' GGGCG ` | ` 5' 
CCCGCNNNN  NN
 3' `
` 3' 
GGGCGNNNNNN  
 5' ` |
SmuEI |  | Streptococcus mutans E | ` 5' GGWCC `
` 3' CCWGG ` | ` 5' 
G  GWCC
 3' `
` 3' 
CCWG  G
 5' ` | Bme216I, CauI, EagMI, FdiI, HgiBI, HgiHIII, Psp03I, VpaK11BI
SnaBI |  | Sphaerotilus natans | ` 5' TACGTA `
` 3' ATGCAT ` | ` 5' 
TAC  GTA
 3' `
` 3' 
ATG  CAT
 5' ` |
SniI |  | Streptomyces niveus | ` 5' CCWGG `
` 3' GGWCC ` | ` 5' 
CC  WGG
 3' `
` 3' 
GGW  CC
 5' ` | ApaORI, BseBI, BshGI, BstNI, BstOI, Bst2UI, BthEI, MvaI, SspAI
SnoI |  | Streptomyces novocastria | ` 5' GTGCAC `
` 3' CACGTG ` | ` 5' 
G  TGCAC
 3' `
` 3' 
CACGT  G
 5' ` | Alw44I, ApaLI, VneI
SolI |  | Streptoverticillium olivoverticillatum | ` 5' GGATCC `
` 3' CCTAGG ` | ` 5' 
G  GATCC
 3' `
` 3' 
CCTAG  G
 5' ` | AccEBI, AliI, ApaCI, BamHI, Bce751I, CelI, OkrAI, SolI, SurI
Sol10179I |  | Streptomyces olivochromogenes ST10179 | ` 5' CTCGAG `
` 3' GAGCTC ` | ` 5' 
C  TCGAG
 3' `
` 3' 
GAGCT  C
 5' ` | BspAAI, MavI, MlaAI, PaeR7I, Sbi68I, Sol10179I, StrI, XpaI
SpaHI |  | Synechococus parietina | ` 5' GCATGC `
` 3' CGTACG ` | ` 5' 
GCATG  C
 3' `
` 3' 
C  GTACG
 5' ` |
SpeI |  | Sphaerotilus natans | ` 5' ACTAGT `
` 3' TGATCA ` | ` 5' 
A  CTAGT
 3' `
` 3' 
TGATC  A
 5' ` | AhII, AclNI, BcuI
SphI[7] |  | Streptomyces phaeochromogenes | ` 5' GCATGC `
` 3' CGTACG ` | ` 5' 
GCATG  C
 3' `
` 3' 
C  GTACG
 5' ` |
SplI |  | Spirulina platensis | ` 5' CGTACG `
` 3' GCATGC ` | ` 5' 
C  GTACG
 3' `
` 3' 
GCATG  C
 5' ` |
SpmI |  | Sphingobacterium multivorum RFL21 | ` 5' ATCGAT `
` 3' TAGCTA ` | ` 5' 
AT  CGAT
 3' `
` 3' 
TAGC  TA
 5' ` | BanIII, BbvAII, BdiI, BscI, Bsa29I, BspJI, ClaI, LcaI, PgaI, Ssp27144I
SpoI |  | Salmonella potsdam | ` 5' TCGCGA `
` 3' AGCGCT ` | ` 5' 
TCG  CGA
 3' `
` 3' 
AGC  GCT
 5' ` |
SpuI |  | Streptomyces pulveraceus | ` 5' CCGCGG `
` 3' GGCGCC ` | ` 5' 
CCGC  GG
 3' `
` 3' 
GG  CGCC
 5' ` |
SrfI |  | Streptomyces sp. | ` 5' GCCCGGGC `
` 3' CGGGCCCG ` | ` 5' 
GCCC  GGGC
 3' `
` 3' 
CGGG  CCCG
 5' ` |
SrlI |  | Selenomonas ruminantium lactilytica 84 | ` 5' GCCGGC `
` 3' CGGCCG ` | ` 5' 
G  CCGGC
 3' `
` 3' 
CGGCC  G
 5' ` |
Srl5DI |  | Selenomonas ruminantium 5 | ` 5' CTGCAG `
` 3' GACGTC ` | ` 5' 
CTGCA  G
 3' `
` 3' 
G  ACGTC
 5' ` | AliAJI, Asp713I, BspMAI, BsuBI, CfrA4I, Pfl21I, PstI, SflI, Srl5DI
Srl32DII |  | Selenomonas ruminantium 32 | ` 5' GAATTC `
` 3' CTTAAG ` | ` 5' 
G  AATTC
 3' `
` 3' 
CTTAA  G
 5' ` |
Srl55DI |  | Selenomonas ruminantium lactilytica 55 | ` 5' GAATTC `
` 3' CTTAAG ` | ` 5' 
G  AATTC
 3' `
` 3' 
CTTAA  G
 5' ` |
Srl56DI |  | Selenomonas ruminantium 56 | ` 5' CTRYAG `
` 3' GAYRTC ` | ` 5' 
C  TRYAG
 3' `
` 3' 
GAYRT  C
 5' ` |
SruI |  | Selenomonas ruminantium 18D | ` 5' TTTAAA `
` 3' AAATTT ` | ` 5' 
TTT  AAA
 3' `
` 3' 
AAA  TTT
 5' ` | AhaIII, DraI, PauAII
Sru4DI |  | Selenomonas ruminantium | ` 5' ATTAAT `
` 3' TAATTA ` | ` 5' 
AT  TAAT
 3' `
` 3' 
TAAT  TA
 5' ` | AseI, AsnI, BpoAI, PshBI, VspI
Sru30DI |  | Selenomonas ruminantium 30 | ` 5' AGGCCT `
` 3' TCCGGA ` | ` 5' 
AGG  CCT
 3' `
` 3' 
TCC  GGA
 5' ` | AatI, AspMI, Eco147I, GdiI, PceI, Pme55I, SarI, SseBI, SteI, StuI
SsbI |  | Streptomyces scabies | ` 5' AAGCTT `
` 3' TTCGAA ` | ` 5' 
A  AGCTT
 3' `
` 3' 
TTCGA  A
 5' ` |
SscL1I |  | Staphylococcus sp. L1 | ` 5' GANTC `
` 3' CTNAG ` | ` 5' 
G  ANTC
 3' `
` 3' 
CTNA  G
 5' ` |
Sse9I |  | Sporosarcina sp. 9 | ` 5' AATT `
` 3' TTAA ` | ` 5' 
  AATT
 3' `
` 3' 
TTAA  
 5' ` |
Sse232I |  | Streptomyces sp. RH232 | ` 5' CGCCGGCG `
` 3' GCGGCCGC ` | ` 5' 
CG  CCGGCG
 3' `
` 3' 
GCGGCC  GC
 5' ` |
Sse1825I |  | Streptomyces sp. | ` 5' GGGWCCC `
` 3' CCCWGGG ` | ` 5' 
GG  GWCCC
 3' `
` 3' 
CCCWG  GG
 5' ` |
Sse8387I |  | Streptomyces sp. 8387 | ` 5' CCTGCAGG `
` 3' GGACGTCC ` | ` 5' 
CCTGCA  GG
 3' `
` 3' 
GG  ACGTCC
 5' ` |
Sse8647I |  | Streptomyces sp. | ` 5' AGGWCCT `
` 3' TCCWGGA ` | ` 5' 
AG  GWCCT
 3' `
` 3' 
TCCWG  GA
 5' ` |
SseAI |  | Streptomyces sp. | ` 5' GGCGCC `
` 3' CCGCGG ` | ` 5' 
GG  CGCC
 3' `
` 3' 
CCGC  GG
 5' ` |
SseBI |  | Streptomyces sp. | ` 5' AGGCCT `
` 3' TCCGGA ` | ` 5' 
AGG  CCT
 3' `
` 3' 
TCC  GGA
 5' ` | AatI, AspMI, Eco147I, GdiI, PceI, Pme55I, SarI, Sru30DI, SteI, StuI
SshAI |  | Salmonella shikmonah | ` 5' CCTNAGG `
` 3' GGANTCC ` | ` 5' 
CC  TNAGG
 3' `
` 3' 
GGANT  CC
 5' ` | AocI, BliHKI, BspR7I, CvnI, Lmu60I, OxaNI, SauI, SshAI
SsiI |  | Staphylococcus sciuri RFL1 | ` 5' CCGC `
` 3' GGCG ` | ` 5' 
C  CGC
 3' `
` 3' 
GGC  G
 5' ` | AciI
SsiAI |  | Staphylococcus sp. A | ` 5' GATC `
` 3' CTAG ` | ` 5' 
  GATC
 3' `
` 3' 
CTAG  
 5' ` | BscFI, BspAI, BstKTI, CpfI, HacI, MkrAI, RalF40I, SauMI
SsiBI |  | Staphylococcus sp. B | ` 5' GATC `
` 3' CTAG ` | ` 5' 
  GATC
 3' `
` 3' 
CTAG  
 5' ` | BscFI, BspFI, BstKTI, CpfI, HacI, MkrAI, RalF40I, SauMI
SslI |  | Streptococcus salivarius thermophilus | ` 5' CCWGG `
` 3' GGWCC ` | ` 5' 
CC  WGG
 3' `
` 3' 
GGW  CC
 5' ` | ApaORI, BseBI, BshGI, BstNI, BstOI, Bst2UI, BthEI, EcoRII, MvaI
SsoI |  | Shigella sonnei 47 | ` 5' GAATTC `
` 3' CTTAAG ` | ` 5' 
G  AATTC
 3' `
` 3' 
CTTAA  G
 5' ` |
SsoII |  | Shigella sonnei 47 | ` 5' CCNGG `
` 3' GGNCC ` | ` 5' 
  CCNGG
 3' `
` 3' 
GGNCC  
 5' ` |
SspI |  | Sphaerotilus sp. | ` 5' AATATT `
` 3' TTATAA ` | ` 5' 
AAT  ATT
 3' `
` 3' 
TTA  TAA
 5' ` |
Ssp1I |  | Streptomyces sp. RFL1 | ` 5' TTCGAA `
` 3' AAGCTT ` | ` 5' 
TT  CGAA
 3' `
` 3' 
AAGC  TT
 5' ` | AsuII, BimI, Bsp119I, CbiI, MlaI, PlaII, SfuI, SspRFI, SviI
Ssp4800I |  | Streptomyces sp. BMTU 4800 | ` 5' TGTACA `
` 3' ACATGT ` | ` 5' 
T  GTACA
 3' `
` 3' 
ACATG  T
 5' ` |  | AauI,  |  BsmRI, | Bsp1407I,

|
|

BsrGI, BstAUI, SspBI
Ssp5230I |  | Streptomyces sp. 5230 | ` 5' GACGTC `
` 3' CTGCAG ` | ` 5' 
GACGT  C
 3' `
` 3' 
C  TGCAG
 5' ` | AatII, ZraI
Ssp27144I |  | Synechococcus sp. | ` 5' ATCGAT `
` 3' TAGCTA ` | ` 5' 
AT  CGAT
 3' `
` 3' 
TAGC  TA
 5' ` | BanIII, BavCI, BbvAII, BscI, BspJI, BspOVII, BstNZ169I, ClaI, LcaI
SspAI |  | Synechococcus sp. | ` 5' CCWGG `
` 3' GGWCC ` | ` 5' 
  CCWGG
 3' `
` 3' 
GGWCC  
 5' ` | AeuI, AorI, Bse17I, BspNI, Bst2I, EcoRII, SniI, SslI, SspAI, ZanI
SspBI |  | Streptomyces sp. | ` 5' TGTACA `
` 3' ACATGT ` | ` 5' 
T  GTACA
 3' `
` 3' 
ACATG  T
 5' ` |  | AauI,  |  BsmRI, | Bsp1407I,

|
|

BsrGI, BstAUI, Ssp4800I
SspCI |  | Streptomyces sp. | ` 5' GCCGGC `
` 3' CGGCCG ` | ` 5' 
GCC  GGC
 3' `
` 3' 
CGG  CCG
 5' ` |
SspD5I |  | Staphylococcus sp. D5 | ` 5' GGTGA `
` 3' CCACT ` | ` 5' 
GGTGAN7N  
 3' `
` 3' 
CCACTN7N  
 5' ` | AsuHPI, HphI
SspD5II |  | Staphylococcus sp. D5 | ` 5' ATGCAT `
` 3' TACGTA ` | ` 5' 
ATGCA  T
 3' `
` 3' 
T  ACGTA
 5' ` | Csp68KIII, EcoT22I, Mph1103I, NsiI, PinBI, Ppu10I, SepI, Zsp2I
SspRFI |  | Synechococcus sp. RF-1 | ` 5' TTCGAA `
` 3' AAGCTT ` | ` 5' 
TT  CGAA
 3' `
` 3' 
AAGC  TT
 5' ` | AsuII, BimI, Bsp119I, CbiI, MlaI, PpaAI, SfuI, Ssp1I, SviI
SsrI |  | Staphylococcus saprophyticus B6 | ` 5' GTTAAC `
` 3' CAATTG ` | ` 5' 
GTT  AAC
 3' `
` 3' 
CAA  TTG
 5' ` |
SstI |  | Streptomyces stanford | ` 5' GAGCTC `
` 3' CTCGAG ` | ` 5' 
GAGCT  C
 3' `
` 3' 
C  TCGAG
 5' ` |
SstII |  | Streptomyces stanford | ` 5' CCGCGG `
` 3' GGCGCC ` | ` 5' 
CCGC  GG
 3' `
` 3' 
GG  CGCC
 5' ` |
Sst12I |  | Streptomyces sp. ST-12 | ` 5' CTGCAG `
` 3' GACGTC ` | ` 5' 
CTGCA  G
 3' `
` 3' 
G  ACGTC
 5' ` | Asp713I, BloHII, BspMAI, BsuBI, CfuII, Psp23I, PstI, SflI, Srl5DI
SteI |  | Streptomyces tendae | ` 5' AGGCCT `
` 3' TCCGGA ` | ` 5' 
AGG  CCT
 3' `
` 3' 
TCC  GGA
 5' ` | AatI, AspMI, Eco147I, GdiI, PceI, Pme55I, SarI, Sru30DI, SseBI, StuI
SthI |  | Salmonella thompson YY356 | ` 5' GGTACC `
` 3' CCATGG ` | ` 5' 
G  GTACC
 3' `
` 3' 
CCATG  G
 5' ` | Acc65I, AhaB8I, Asp718I, KpnI,
Sth117I |  | Streptococcus thermophilus ST117 | ` 5' CCWGG `
` 3' GGWCC ` | ` 5' 
CC  WGG
 3' `
` 3' 
GGW  CC
 5' ` | ApaORI, BseBI, BsiLI, BstNI, BstOI, Bst2UI, EcoRII, MvaI, SspAI
Sth132I |  | Streptococcus thermophilus | ` 5' CCCG `
` 3' GGGC ` | ` 5' 
CCCGNNNN  NNNN
 3' `
` 3' 
GGGCNNNNNNNN  
 5' ` |
Sth134I |  | Streptococcus thermophilus 134 | ` 5' CCGG `
` 3' GGCC ` | ` 5' 
C  CGG
 3' `
` 3' 
GGC  C
 5' ` |
Sth368I |  | Streptococcus thermophilus CNRZ368 | ` 5' GATC `
` 3' CTAG ` | ` 5' 
  GATC
 3' `
` 3' 
CTAG  
 5' ` | BscFI, BspFI, BstKTI, CpfI, HacI, MkrAI, RalF40I, SauMI
StrI |  | Streptomyces thermodiastaticus | ` 5' CTCGAG `
` 3' GAGCTC ` | ` 5' 
C  TCGAG
 3' `
` 3' 
GAGCT  C
 5' ` | AbrI, BluI, BssHI, MavI, Sau3239I, Sfr274I, TliI, XhoI
StsI |  | Streptococcus sanguis 54 | ` 5' GGATG `
` 3' CCTAC ` | ` 5' 
GGATGN8NN  NNNN
 3' `
` 3' 
CCTACN8NNNNNN  
 5' ` |
StuI[8] |  | Streptomyces tubercidicus | ` 5' AGGCCT `
` 3' TCCGGA ` | ` 5' 
AGG  CCT
 3' `
` 3' 
TCC  GGA
 5' ` | AatI, AspMI, Eco147I, GdiI, PceI, SarI, Sru30DI, SseBI, SteI
StyI |  | Salmonella typhi | ` 5' CCWWGG `
` 3' GGWWCC ` | ` 5' 
C  CWWGG
 3' `
` 3' 
GGWWC  C
 5' ` |
StyD4I |  | Salmonella typhi D4 | ` 5' CCNGG `
` 3' GGNCC ` | ` 5' 
  CCNGG
 3' `
` 3' 
GGNCC  
 5' ` |
SuaI |  | Sulfolobus acidocaldarius | ` 5' GGCC `
` 3' CCGG ` | ` 5' 
GG  CC
 3' `
` 3' 
CC  GG
 5' ` |
SuiI |  | Sulfolobus islandicus REN2HI | ` 5' GCWGC `
` 3' CGWCG ` | ` 5' 
G  CWGC
 3' `
` 3' 
 CGWC  G
 5' ` | AceI, ApeKI, Taq52I, TseI
SunI |  | Synechococcus uniformis | ` 5' CGTACG `
` 3' GCATGC ` | ` 5' 
C  GTACG
 3' `
` 3' 
GCATG  C
 5' ` |
SurI |  | Sporosarcina ureae 2 | ` 5' GGATCC `
` 3' CCTAGG ` | ` 5' 
G  GATCC
 3' `
` 3' 
CCTAG  G
 5' ` | AccEBI, AliI, ApaCI, AsiI, BamHI, Bce751I, OkrAI, RspLKII, SolI
SviI |  | Streptomyces violochromogenes | ` 5' TTCGAA `
` 3' AAGCTT ` | ` 5' 
TT  CGAA
 3' `
` 3' 
AAGC  TT
 5' ` | AsuII, Bpu14I, Bsp119I, CbiI, MlaI, PpaAI, SfuI, Ssp1I
SwaI |  | Staphylococcus warneri | ` 5' ATTTAAAT `
` 3' TAAATTTA ` | ` 5' 
ATTT  AAAT
 3' `
` 3' 
TAAA  TTTA
 5' ` |

This list is incomplete; you can help by adding missing items. (February 2011)

|

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## Notes

[edit]

1. ^ Smith HO, Nathans D (December 1973). "Letter: A suggested nomenclature for bacterial host modification and restriction systems and their enzymes". J. Mol. Biol. 81 (3): 419–23\. doi:10.1016/0022-2836(73)90152-6. PMID 4588280.419-23&rft.date=1973-12&rft_id=info:doi/10.1016/0022-2836(73)90152-6&rft_id=info:pmid/4588280&rft.aulast=Smith&rft.aufirst=HO&rft.au=Nathans,+D&rfr_id=info:sid/en.wikipedia.org:List+of+restriction+enzyme+cutting+sites:+S" class="Z3988">
2. ^ Roberts RJ, Belfort M, Bestor T, Bhagwat AS, Bickle TA, Bitinaite J, Blumenthal RM, Degtyarev SK, Dryden DT, Dybvig K, Firman K, Gromova ES, Gumport RI, Halford SE, Hattman S, Heitman J, Hornby DP, Janulaitis A, Jeltsch A, Josephsen J, Kiss A, Klaenhammer TR, Kobayashi I, Kong H, Krüger DH, Lacks S, Marinus MG, Miyahara M, Morgan RD, Murray NE, Nagaraja V, Piekarowicz A, Pingoud A, Raleigh E, Rao DN, Reich N, Repin VE, Selker EU, Shaw PC, Stein DC, Stoddard BL, Szybalski W, Trautner TA, Van Etten JL, Vitor JM, Wilson GG, Xu SY (April 2003). "A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes". Nucleic Acids Res. 31 (7): 1805–12\. doi:10.1093/nar/gkg274. PMC 152790. PMID 12654995.1805-12&rft.date=2003-04&rft_id=https://www.ncbi.nlm.nih.gov/pmc/articles/PMC152790#id-name=PMC&rft_id=info:pmid/12654995&rft_id=info:doi/10.1093/nar/gkg274&rft.aulast=Roberts&rft.aufirst=RJ&rft.au=Belfort,+M&rft.au=Bestor,+T&rft.au=Bhagwat,+AS&rft.au=Bickle,+TA&rft.au=Bitinaite,+J&rft.au=Blumenthal,+RM&rft.au=Degtyarev,+SK&rft.au=Dryden,+DT&rft.au=Dybvig,+K&rft.au=Firman,+K&rft.au=Gromova,+ES&rft.au=Gumport,+RI&rft.au=Halford,+SE&rft.au=Hattman,+S&rft.au=Heitman,+J&rft.au=Hornby,+DP&rft.au=Janulaitis,+A&rft.au=Jeltsch,+A&rft.au=Josephsen,+J&rft.au=Kiss,+A&rft.au=Klaenhammer,+TR&rft.au=Kobayashi,+I&rft.au=Kong,+H&rft.au=Krüger,+DH&rft.au=Lacks,+S&rft.au=Marinus,+MG&rft.au=Miyahara,+M&rft.au=Morgan,+RD&rft.au=Murray,+NE&rft.au=Nagaraja,+V&rft.au=Piekarowicz,+A&rft.au=Pingoud,+A&rft.au=Raleigh,+E&rft.au=Rao,+DN&rft.au=Reich,+N&rft.au=Repin,+VE&rft.au=Selker,+EU&rft.au=Shaw,+PC&rft.au=Stein,+DC&rft.au=Stoddard,+BL&rft.au=Szybalski,+W&rft.au=Trautner,+TA&rft.au=Van+Etten,+JL&rft.au=Vitor,+JM&rft.au=Wilson,+GG&rft.au=Xu,+SY&rft_id=https://www.ncbi.nlm.nih.gov/pmc/articles/PMC152790&rfr_id=info:sid/en.wikipedia.org:List+of+restriction+enzyme+cutting+sites:+S" class="Z3988">
3. ^ Jeremy MB, John LT, Lubert S (2002). "3. Protein Structure and Function". Biochemistry. San Francisco: W. H. Freeman. ISBN 0-7167-4684-0.
4. ^ Anfinsen C.B. (1973). "Principles that Govern the Folding of Protein Chains". Science. 181 (4096): 223–30\. doi:10.1126/science.181.4096.223. PMID 4124164.223-30&rft.date=1973&rft_id=info:doi/10.1126/science.181.4096.223&rft_id=info:pmid/4124164&rft.au=Anfinsen+C.B.&rfr_id=info:sid/en.wikipedia.org:List+of+restriction+enzyme+cutting+sites:+S" class="Z3988">
5. ^ Kessler C, Manta V (August 1990). "Specificity of restriction endonucleases and DNA modification methyltransferases a review (Edition 3)". Gene. 92 (1–2): 1–248\. doi:10.1016/0378-1119(90)90486-B. PMID 2172084.1–2&rft.pages=1-248&rft.date=1990-08&rft_id=info:doi/10.1016/0378-1119(90)90486-B&rft_id=info:pmid/2172084&rft.aulast=Kessler&rft.aufirst=C&rft.au=Manta,+V&rfr_id=info:sid/en.wikipedia.org:List+of+restriction+enzyme+cutting+sites:+S" class="Z3988">
6. ^ Pingoud A, Jeltsch A (September 2001). "Structure and function of type II restriction endonucleases". Nucleic Acids Res. 29 (18): 3705–27\. doi:10.1093/nar/29.18.3705. PMC 55916. PMID 11557805.3705-27&rft.date=2001-09&rft_id=https://www.ncbi.nlm.nih.gov/pmc/articles/PMC55916#id-name=PMC&rft_id=info:pmid/11557805&rft_id=info:doi/10.1093/nar/29.18.3705&rft.aulast=Pingoud&rft.aufirst=A&rft.au=Jeltsch,+A&rft_id=https://www.ncbi.nlm.nih.gov/pmc/articles/PMC55916&rfr_id=info:sid/en.wikipedia.org:List+of+restriction+enzyme+cutting+sites:+S" class="Z3988">
7. ^ a b c d Monty Krieger; Matthew P Scott; Matsudaira, Paul T.; Lodish, Harvey F.; Darnell, James E.; Lawrence Zipursky; Kaiser, Chris; Arnold Berk (2004). Molecular Cell Biology (5th ed.). New York: W.H. Freeman and Company. ISBN 0-7167-4366-3.
8. ^ Shimotsu H, Takahashi H, Saito H (November 1980). "A new site-specific endonuclease StuI from Streptomyces tubercidicus". Gene. 11 (3–4): 219–25\. doi:10.1016/0378-1119(80)90062-1. PMID 6260571.3–4&rft.pages=219-25&rft.date=1980-11&rft_id=info:doi/10.1016/0378-1119(80)90062-1&rft_id=info:pmid/6260571&rft.aulast=Shimotsu&rft.aufirst=H&rft.au=Takahashi,+H&rft.au=Saito,+H&rfr_id=info:sid/en.wikipedia.org:List+of+restriction+enzyme+cutting+sites:+S" class="Z3988">

* v
* t
* e

Restriction modification system: restriction enzyme

Basic Concept|

* Isoschizomer
* Neoschizomer
* Isocaudomer

Recognition sequence 4bp|

* TaqI
* Sau3A
* AluI*
* HaeIII*
* DpnII
* NlaIII
* HpaII

Recognition sequence 5bp|

* EcoRII
* FokI

Recognition sequence 6bp|

* AaaI
* BglII
* PovII*
* EcoRV*
* EcoRI
* BamHI
* HindIII
* XbaI
* XhoI
* SacI
* PstI
* NdeI

Recognition sequence 8bp|

* NotI

Lists|

* A
* Ba–Bc
* Bd–Bp
* Bsa–Bso
* Bsp–Bss
* Bst–Bv
* C-D
* E-F
* G-K
* L-N
* O-R
* S
* T-Z

* means cleavage produces blunt ends
*[v]: View this template
*[t]: Discuss this template
*[e]: Edit this template