MMN medium

Agar media

MMN medium or Modified Melin-Norkrans medium is a type of agar growth medium, used to grow cultures of mycorrhizal fungi, such as Boletus edulis and Tricholoma matsutake.[1][2][3][4][5] It was first described by DH. Marx in The influence of ectotrophic mycorrhizal fungi on the resistance of pine roots to pathogenic infections. I. Antagonism of mycorrhizal fungi to root pathogenic fungi and soil bacteria in 1969.[1] The acidic pH (5.6) of MMN agar inhibits bacterial growth.[6]

## Typical composition

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MMN agar typically contains:[6]

* 10 g/L glucose
* 3 g/L malt extract
* 0.25 g/L (NH4)2HPO4
* 0.025 g/L NaCl
* 0.5 g/L KH2PO4
* 0.05 g/L CaCl2
* 0.15 g/L MgSO4.7 H2O
* 0.012g/L FeCl3. 6 H2O
* 0.003 g/L thiamine
* 15 g/L agar

## References

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1. ^ a b Águeda, Beatriz; Parladé, Javier; Fernández-Toirán, Luz Marina; Cisneros, Óscar; de Miguel, Ana María; Modrego, María Pilar; Martínez-Peña, Fernando; Pera, Joan (October 2008). "Mycorrhizal synthesis between Boletus edulis species complex and rockroses (Cistus sp.)". Mycorrhiza. 18 (8): 443–449\. Bibcode:2008Mycor..18..443A. doi:10.1007/s00572-008-0192-3. ISSN 0940-6360. PMID 18695982. S2CID 11233267.
2. ^ Mediavilla, Olaya; Olaizola, Jaime; Santos-del-Blanco, Luis; Oria-de-Rueda, Juan Andrés; Martín-Pinto, Pablo (February 2016). "Mycorrhization between Cistus ladanifer L. and Boletus edulis Bull is enhanced by the mycorrhiza helper bacteria Pseudomonas fluorescens Migula". Mycorrhiza. 26 (2): 161–168\. Bibcode:2016Mycor..26..161M. doi:10.1007/s00572-015-0657-0. ISSN 0940-6360. PMID 26208816. S2CID 254061088.
3. ^ Islam, Ferzana; Ohga, Shoji (2013-03-12). "Effects of Media Formulation on the Growth and Morphology of Ectomycorrhizae and Their Association with Host Plant". International Scholarly Research Notices. 2013: e317903. doi:10.1155/2013/317903.
4. ^ Chaitali Bhattacharya; Alok Adholeya. "Showcasing Ectomycorrhizal Culture". bookstore.teri.res.in. Retrieved 2023-02-04.
5. ^ Declerck, Stéphane; Strullu, D. G.; Fortin, André (2005-04-13). In Vitro Culture of Mycorrhizas. Springer Science & Business Media. p. 259\. ISBN 978-3-540-24027-3.
6. ^ a b "CBS List of Media" (PDF).

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Growth media / agar plates

Selective media|

| Gram positive|

| Actinomycetota|

* Mycobacterium tuberculosis
* Löwenstein–Jensen medium
* Middlebrook 7H9 Broth
* Middlebrook 7H10 Agar
* Middlebrook 7H11 Agar
* Mycoplasma pneumoniae
* Eaton's agar

|

Bacillota|

* Corynebacterium diphtheriae
* Hoyle's agar
* Enterococcus
* Bile esculin agar
* Lactobacillus
* MRS agar
* Lactococcus
* M17 agar
* Staphylococcus
* Mannitol salt agar
* Baird-Parker agar
* Vogel–Johnson agar

Gram negative|

| Alphaproteobacteria|

* Brucella
* Brucella agar
* Farrell's medium

|

Betaproteobacteria|

* Neisseria
* Thayer–Martin agar
* New York City agar

Gammaproteobacteria|

* Aggregatibacter actinomycetemcomitans
* Tryptic soy-serum-bacitracin-vancomycin
* Bordetella
* Bordet–Gengou agar
* Enterobacteriaceae
* VRBD agar
* Haemophilus influenzae/Legionella pneumophila
* Buffered charcoal yeast extract agar
* Pseudomonas aeruginosa
* Cetrimide agar
* Salmonella
* XLT agar
* DCA agar
* Salmonella/Shigella
* XLD agar

Differential media|

* Lactose fermenting gram negative
* MacConkey agar/Sorbitol-MacConkey agar
* Eosin methylene blue
* Endo agar
* Bismuth sulfite agar
* Hektoen enteric agar
* Lysine iron agar
* Simmons' citrate agar
* TSI slant

Fungal media|

* BAF agar
* Czapek medium
* Dermatophyte test medium
* MMN medium
* Potato dextrose agar
* Sabouraud agar
* YEPD
* YM

Nonselective media|

* Blood agar
* Chocolate agar
* Lysogeny broth
* Nutrient agar
* Plate count agar
* Trypticase soy agar
* Tryptic soy broth

Other/ungrouped media|

* Brain heart infusion
* Cystine–lactose–electrolyte-deficient agar
* Cystine tryptic agar
* Glucose phosphate broth
* Lauryl tryptose broth
* Mannitol motility medium
* Mueller–Hinton agar/PNP agar
* R2A agar
* Schädler agar

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