Neanderthal genome project

Effort to sequence the Neanderthal genome

The Neanderthal genome project is an effort, founded in July 2006, of a group of scientists to sequence the Neanderthal genome.

It was initiated by 454 Life Sciences, a biotechnology company based in Branford, Connecticut in the United States and is coordinated by the Max Planck Institute for Evolutionary Anthropology in Germany. In May 2010 the project published their initial draft of the Neanderthal genome (Vi33.16, Vi33.25, Vi33.26) based on the analysis of four billion base pairs of Neanderthal DNA. The study determined that some mixture of genes occurred between Neanderthals and anatomically modern humans and presented evidence that elements of their genome remain in modern humans outside Africa.[1][2][3]

In December 2013, a high coverage genome of a Neanderthal was reported for the first time. DNA was extracted from a toe fragment from a female Neanderthal researchers have dubbed the "Altai Neandertal". It was found in Denisova Cave in the Altai Mountains of Siberia and is estimated to be 50,000 years old.[4][5]

## Findings

[edit]

The researchers recovered ancient DNA of Neanderthals by extracting the DNA from the femur bones of three 38,000 year-old female specimens from Vindija Cave, Croatia, and other bones found in Spain, Russia, and Germany.[6] Only about half a gram of the bone samples (or 21 samples each 50–100 mg[1]) was required for the sequencing, but the project faced many difficulties, including the contamination of the samples by the bacteria that had colonized the specimen's body and humans who handled the bones at the excavation site and at the laboratory.[7]

Svante Pääbo, director of the Department of Genetics at the Max Planck Institute for Evolutionary Anthropology and head of its Neanderthal genome project

In February 2009, the Max Planck Institute's team led by Svante Pääbo announced that they had completed the first draft of the Neanderthal genome.[7] An early analysis of the data suggested in "the genome of Neanderthals, a human species driven to extinction" "no significant trace of Neanderthal genes in modern humans".[8] New results suggested that some adult specimens were lactose intolerant.[9] On the question of potentially cloning a Neanderthal, Pääbo commented, "Starting from the DNA extracted from a fossil, it is and will remain impossible."[7]

In May 2010, the project released a draft of their report on the sequenced genome. Contradicting the results discovered while examining mitochondrial DNA (mtDNA), they demonstrated a wide range of genetic contribution to non-African modern humans ranging from 1% to 4%. From their Homo sapiens samples in Eurasia (French, Han Chinese and Papuan) the authors stated that it is likely that interbreeding occurred in the Levant before Homo sapiens migrated into Europe.[10] This finding is disputed because of the paucity of archeological evidence supporting their statement. The fossil evidence does not conclusively place Neanderthals and modern humans in close proximity at this time and place, thus they couldn't share DNA unless they were a part of the same species.[11] According to preliminary sequences from 2010, 99.7% of the nucleotide sequences of the modern human and Neanderthal genomes are identical, compared to humans sharing around 94% of sequences with the chimpanzee.[12] (For some time, studies concerning the commonality between chimps and humans modified the commonality of 99% to a commonality of 94%, showing that the genetic gap between humans and chimpanzees was far larger than originally thought,[13][14] but other studies suggest the difference between humans, chimpanzees, and bonobos is just about 1.0–1.2%.[15][16])

Additionally, in 2010, the discovery and analysis of mtDNA from the Denisova hominin in Siberia revealed that it differed from that of modern humans by 385 bases (nucleotides) in the mtDNA strand out of approximately 16,500, whereas the difference between modern humans and Neanderthals is just 202 bases. In contrast, the difference between chimpanzees and modern humans is approximately 1,462 mtDNA base pairs. Analysis of the specimen's nuclear DNA was then still under way and expected to clarify whether or not the find is a distinct species.[17][18] Even though the Denisova hominin's mtDNA lineage predates the divergence of modern humans and Neanderthals, coalescent theory does not preclude a more recent divergence date for her nuclear DNA.

A rib fragment from the partial skeleton of an infant found in the Mezmaiskaya cave in the northwestern foothills of the Caucasus Mountains was radiocarbon-dated in 1999 to 29,195±965 B.P., and therefore must be a Neanderthal. Ancient DNA recovered for a mtDNA sequence showed 3.48% divergence from that of the Feldhofer Neanderthal, some 2,500 km to the west in Germany and in 2011 Phylogenetic analysis placed the two in a clade distinct from modern humans, suggesting that their mtDNA types may not have contributed to the modern human mtDNA pool.[19]

In 2015, Israel Hershkovitz of Tel Aviv University reported that a skull found in a cave in northern Israel, is "probably a woman, who lived and died in the region about 55,000 years ago, placing modern humans there and then for the first time ever", pointing to a potential time and location when modern humans first interbred with Neanderthals if present.[20]

In 2016, the project found that Neanderthals mated with modern humans multiple times, and that Neanderthals interbred with Denisovans only once, as evidenced in the genome of modern-day Melanesians.[21]

In 2006, two research teams working on the same Neanderthal sample published their results, Richard Green and his team in Nature,[22] and James Noonan's team in Science.[23] The results were received with some scepticism, mainly surrounding the issue of a possible admixture of Neanderthals into the modern human genome.[24]

In 2006, Richard Green's team had used a then new sequencing technique developed by 454 Life Sciences that amplifies single molecules for characterization and obtained over a quarter of a million unique short sequences ("reads"). The technique delivers randomly located reads, so that sequences of interest – genes that differ between modern humans and Neanderthals – show up at random as well. However, this form of direct sequencing destroys the original sample so to obtain new reads more samples must be destructively sequenced.[25]

Noonan's team, led by Edward Rubin, used a different technique, one in which the DNA is inserted into bacteria, which make multiple copies of a single fragment. They demonstrated that Neanderthal genomic sequences can be recovered using a metagenomic library-based approach. All of the DNA in the sample is "immortalized" into metagenomic libraries. A DNA fragment is selected, then propagated in microbes. The resulting Neanderthal DNA sequences can then be sequenced or specific sequences can be studied.[25]

Overall, their results were similar. One group suggested there was mixing between human and Neanderthal genomes, while the other found none, but both teams recognized that the data set was not large enough to give a definitive answer.[24]

The publication by Noonan, and his team revealed Neanderthal DNA sequences matching chimpanzee DNA, but not modern human DNA, at some locations, thus enabling the first rough calculation of the date of the most recent common ancestor of H. sapiens and H. neanderthalensis. The research team estimates the most recent common ancestor of their H. neanderthalensis samples and their H. sapiens reference sequence lived 706,000 years ago (divergence time), estimating the separation of the human and Neanderthal ancestral populations to 370,000 years ago (split time).

> Our analyses suggest that on average the Neanderthal genomic sequence we obtained and the reference human genome sequence share a most recent common ancestor ~706,000 years ago, and that the human and Neanderthal ancestral populations split ~370,000 years ago, before the emergence of anatomically modern humans.

> — Noonan et al. (2006)[23]

Based on the analysis of mitochondrial DNA, the split of the Neanderthal and H. sapiens lineages is estimated to date to between 760,000 and 550,000 years ago (95% CI).[26]

Mutations of the speech-related gene FOXP2 identical to those in modern humans were discovered in Neanderthal DNA from the El Sidrón 1253 and 1351c specimens,[27] suggesting Neanderthals might have shared some language capabilities with modern humans.[9]

## See also

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* Cro-Magnon – Earliest anatomically modern humans in EuropePages displaying short descriptions of redirect targets
* Neanderthal extinction
* Interbreeding between archaic and modern humans – Evidence of human hybridization during the Paleolithic

General:

* Admixture mapping – Result of interbreeding between two or more previously isolated populations within a speciesPages displaying short descriptions of redirect targets
* Gene flow – Transfer of genetic variation from one population to another
* Hybrid – Offspring of cross-species reproduction

## References

[edit]

1. ^ a b Green, R. E.; Krause, J.; Briggs, A. W.; et al. (May 2010). "A draft sequence of the Neandertal genome" (PDF). Science. 328 (5979): 710–22\. Bibcode:2010Sci...328..710G. doi:10.1126/science.1188021. PMC 5100745. PMID 20448178.
2. ^ "The Neanderthal in Us" (PDF) (Press release). Max Planck Institute for Evolutionary Anthropology. Archived from the original (PDF) on 2010-05-16. Retrieved 2010-05-08.
3. ^ "Neandertal DNA may raise risk for some modern human diseases". Science News. 11 February 2016. Retrieved 15 February 2016.
4. ^ Zimmer, Carl (18 December 2013). "Toe fossil provides complete Neanderthal genome". New York Times. Retrieved 18 December 2013.
5. ^ Prüfer, Kay; et al. (18 December 2013). "The complete genome sequence of a Neanderthal from the Altai Mountains". Nature. 505 (7481): 43–49\. Bibcode:2014Natur.505...43P. doi:10.1038/nature12886. PMC 4031459. PMID 24352235.
6. ^ "Scientists decode majority of Neanderthal man's genome". Deutsche Welle. 13 February 2009.
7. ^ a b c McGroarty, Patrick (12 February 2009). "Team in Germany maps Neanderthal genome". The Associated Press. Archived from the original on 26 September 2012. Retrieved 2 April 2011.
8. ^ Wade, Nicholas (12 February 2009). "Scientists in Germany draft Neanderthal genome". The New York Times. Retrieved 20 May 2010.
9. ^ a b Inman, Mason (12 February 2009). "Neanderthal genome "first draft" unveiled". National Geographic News. Archived from the original on February 16, 2009.
10. ^ Green, R. E.; Krause, J.; Briggs, A. W.; et al. (7 May 2010). "Draft full sequence of Neanderthal Genome". Science. 328 (5979): 710–22\. Bibcode:2010Sci...328..710G. doi:10.1126/science.1188021. PMC 5100745. PMID 20448178.`{{cite journal}}`: CS1 maint: multiple names: authors list (link)
11. ^ Wade, Nicholas (6 May 2010). "Signs of Neanderthals mating with humans". The New York Times.
12. ^ Than, Ker (6 May 2010). "Neanderthals, humans interbred – first solid DNA evidence". National Geographic Society. Archived from the original on May 9, 2010. Retrieved 9 May 2010.
13. ^ Cohen, Jon (29 June 2007). "Relative differences: the myth of 1%" (PDF). AAAS.
14. ^ "Humans and chimps: close but not that close". Scientific American. 19 December 2006. Archived from the original on 11 October 2007. Retrieved 20 December 2006.
15. ^ Wong, Kate (1 September 2014). "Tiny genetic differences between humans and other primates pervade the genome". Scientific American. Retrieved 12 October 2016.
16. ^ Gibbons, Ann (13 June 2012). "Bonobos join chimps as closest human relatives". Science/AAAS.
17. ^ Brown, David (25 March 2010). "DNA from bone shows new human forerunner, and raises array of questions". Washington Post.
18. ^ Krause, J.; Fu, Q.; Good, J. M.; et al. (April 2010). "The complete mitochondrial DNA genome of an unknown hominin from southern Siberia". Nature. 464 (7290): 894–97\. Bibcode:2010Natur.464..894K. doi:10.1038/nature08976. PMC 10152974. PMID 20336068.
19. ^ Ovchinnikov, Igor V.; Götherström, Anders; Romanova, Galina P.; Kharitonov, Vitaliy M.; Lidén, Kerstin; Goodwin, William (30 March 2000). "Molecular analysis of Neanderthal DNA from the northern Caucasus". Nature. 404 (6777): 490–93\. Bibcode:2000Natur.404..490O. doi:10.1038/35006625. PMID 10761915. S2CID 3101375.
20. ^ "Skull discovery suggests location where humans first had sex with Neanderthals". The Guardian. 28 January 2015.
21. ^ Vernot B.; Tucci S.; Kelso J.; et al. (2016). "Excavating Neandertal and Denisovan DNA from the genomes of Melanesian individuals". Science. 352 (6282): 235–39\. Bibcode:2016Sci...352..235V. doi:10.1126/science.aad9416. PMC 6743480. PMID 26989198.`{{cite journal}}`: CS1 maint: multiple names: authors list (link)
22. ^ Green, Richard E.; et al. (16 November 2006). "Analysis of one million base pairs of Neanderthal DNA" (PDF). Nature. 444 (7117): 330–36\. Bibcode:2006Natur.444..330G. doi:10.1038/nature05336. PMID 17108958.
23. ^ a b Noonan, James P.; et al. (17 November 2006). "Sequencing and analysis of Neanderthal genomic DNA" (PDF). Science. 314 (5802): 1113–18\. Bibcode:2006Sci...314.1113N. doi:10.1126/science.1131412. PMC 2583069. PMID 17110569. Archived from the original (PDF) on 17 July 2011. Retrieved 9 July 2008.
24. ^ a b Timmer, John (17 November 2006). "Welcome to Neanderthal genomics". ArsTechnica.
25. ^ a b Yarris, Lynn (15 November 2006). "Neanderthal genome sequencing yields surprising results and opens a new door to future studies". Lawrence Berkeley National Laboratory. Archived from the original on 10 March 2009. Retrieved 16 February 2009.
26. ^ Cosimo, Posth; et al. (2017). "Deeply divergent archaic mitochondrial genome provides lower time boundary for African gene flow into Neanderthals". Nature Communications. 8: 16046. Bibcode:2017NatCo...816046P. doi:10.1038/ncomms16046. PMC 5500885. PMID 28675384.
27. ^ Krause, J.; Lalueza-Fox, C.; Orlando, L.; et al. (November 2007). "The derived FOXP2 variant of modern humans was shared with Neandertals". Current Biology. 17 (21): 1908–12\. Bibcode:2007CBio...17.1908K. doi:10.1016/j.cub.2007.10.008. hdl:11858/00-001M-0000-000F-FED3-1. PMID 17949978. S2CID 9518208.

## Further reading

[edit]

* Green, Richard E.; et al. (2008-08-08). "A Complete Neandertal Mitochondrial Genome Sequence Determined by High-Throughput Sequencing" (PDF). Cell. 134 (3). Elsevier: 416–26\. doi:10.1016/j.cell.2008.06.021. PMC 2602844. PMID 18692465. Archived from the original (PDF) on 2008-12-17.

## External links

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* "The Neandertal Genome Project Website"
* Neanderthal genome project on National Public Radio
* "Genetic algorithm model shows that modern humans out-competed the Neanderthal" Short summary of a peer review paper 2008
* "Last of the Neanderthals" National Geographic, October 2008
* MSNBC "Neanderthal genome project launches" 22 November 2006.
* BBC News Paul Rincon, "Neanderthal DNA secrets unlocked" 15 November 2006
* "Welcome to Neanderthal genomics" 17 November 2006
* "Neanderthal Genome Sequencing Yields Surprising Results and Opens a New Door to Future Studies" Archived 2009-03-10 at the Wayback Machine 15 November 2006

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