NotI

Restriction enzyme

NotI is a restriction enzyme isolated from the bacterium Nocardia otitidis.[1]

## Recognition sequence

[edit]

Palindromic recognition sequence of NotI:[1]

5'-GCGGCCGC-3'
3'-CGCCGGCG-5'

The ends generated by NotI digest:

5'
GC     GGCCGC
3'
3'
CGCCGG     CG
5'

## References

[edit]

1. ^ a b Biolabs, New England. "NotI | NEB". www.neb.com. Retrieved 2016-05-08.

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Restriction modification system: restriction enzyme

Basic Concept|

* Isoschizomer
* Neoschizomer
* Isocaudomer

Recognition sequence 4bp|

* TaqI
* Sau3A
* AluI*
* HaeIII*
* DpnII
* NlaIII
* HpaII

Recognition sequence 5bp|

* EcoRII
* FokI

Recognition sequence 6bp|

* AaaI
* BglII
* PovII*
* EcoRV*
* EcoRI
* BamHI
* HindIII
* XbaI
* XhoI
* SacI
* PstI
* NdeI

Recognition sequence 8bp|

* NotI

Lists|

* A
* Ba–Bc
* Bd–Bp
* Bsa–Bso
* Bsp–Bss
* Bst–Bv
* C-D
* E-F
* G-K
* L-N
* O-R
* S
* T-Z

* means cleavage produces blunt ends

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