Saline (medicine)

Saline water for medical purposes

Saline

Saline solution for intravenous infusion

Clinical data
AHFS/Drugs.com| FDA Professional Drug Information
License data|

* US DailyMed: Hypertonic_Saline

Routes of
administration| Intravenous, topical, subcutaneous
ATC code|

* B05XA03 (WHO)

Identifiers
CAS Number|

* 7647-14-5

PubChem CID|

* 5234

DrugBank|

* DB09153

ChemSpider|

* 5044

UNII|

* 451W47IQ8X

KEGG|

* C13563

ChEBI|

* CHEBI:26710

ChEMBL|

* ChEMBL1200574

Chemical and physical data
Formula| ClNa
Molar mass| 58.44 g·mol−1
3D model (JSmol)|

* Interactive image

SMILES

* [Na+].[Cl-]

InChI

* InChI=1S/ClH.Na/h1H;/q;+1/p-1

* Key:FAPWRFPIFSIZLT-UHFFFAOYSA-M

Saline (also known as saline solution) is a mixture of sodium chloride (salt) and water.[1] It has a number of uses in medicine including cleaning wounds, removal and storage of contact lenses, and help with dry eyes.[2] By injection into a vein, it is used to treat hypovolemia such as that from gastroenteritis and diabetic ketoacidosis.[2][1] Large amounts may result in fluid overload, swelling, acidosis, and high blood sodium.[1][2] In those with long-standing low blood sodium, excessive use may result in osmotic demyelination syndrome.[2]

Saline is in the crystalloid family of medications.[3] It is most commonly used as a sterile 9 g of salt per litre (0.9%) solution, known as normal saline.[1] Higher and lower concentrations may also occasionally be used.[4][5] Saline is acidic, with a pH of 5.5 (due mainly to dissolved carbon dioxide).[6]

The medical use of saline began around 1831.[7] It is on the World Health Organization's List of Essential Medicines.[8] In 2022, sodium salts was the 216th most commonly prescribed medication in the United States, with more than 1 million prescriptions.[9][10]

## Normal concentration

[edit]

Saline solution for irrigation

Normal saline (NSS, NS or N/S) is the commonly used phrase for a solution of 0.90% w/v of NaCl, 308 mOsm/L or 9.0 g per liter. Less commonly, this solution is referred to as physiological saline or isotonic saline (because it is approximately isotonic to blood serum, which makes it a physiologically normal solution). Although neither of those names is technically accurate because normal saline is not exactly like blood serum, they convey the practical effect usually seen: good fluid balance with minimal hypotonicity or hypertonicity. NS is used frequently in intravenous drips (IVs) for people who cannot take fluids orally and have developed or are in danger of developing dehydration or hypovolemia. NS is also used for aseptic purpose. NS is typically the first fluid used when hypovolemia is severe enough to threaten the adequacy of blood circulation, and has long been believed to be the safest fluid to give quickly in large volumes. However, it is now known that rapid infusion of NS can cause metabolic acidosis.[11]

The solution is 9 grams of sodium chloride (NaCl) dissolved in water, to a total volume of 1000 ml (weight per unit volume). The mass of 1 millilitre of normal saline is 1.0046 grams at 22 °C.[12][13] The molecular weight of sodium chloride is approximately 58.4 grams per mole, so 58.4 grams of sodium chloride equals 1 mole. Since normal saline contains 9 grams of NaCl, the concentration is 9 grams per litre divided by 58.4 grams per mole, or 0.154 mole per litre. Since NaCl dissociates into two ions – sodium and chloride – 1 molar NaCl is 2 osmolar. Thus, NS contains 154 mEq/L of Na+ and the same amount of Cl−. This points to an osmolarity of 154 + 154 = 308, which is higher (i.e. more solute per litre) than that of blood (approximately 285).[14] However, if the osmotic coefficient (a correction for non-ideal solutions) is taken into account, then the saline solution is much closer to isotonic. The osmotic coefficient of NaCl is about 0.93,[15] which yields an osmolarity of 0.154 × 1000 × 2 × 0.93 = 286.44. Therefore, the osmolarity of normal saline is a close approximation to the osmolarity of blood.

### Usage

[edit]

For medical purposes, saline is often used to flush wounds and skin abrasions. However, research indicates that it is no more effective than potable tap water.[16] Normal saline will not burn or sting when applied.[citation needed]

Saline is also used in I.V. therapy, intravenously supplying extra water to rehydrate people or supplying the daily water and salt needs ("maintenance" needs) of a person who is unable to take them by mouth. Because infusing a solution of low osmolality can cause problems such as hemolysis, intravenous solutions with reduced saline concentrations (less than 0.9%) typically have dextrose (glucose) added to maintain a safe osmolality while providing less sodium chloride. The amount of normal saline infused depends largely on the needs of the person (e.g. ongoing diarrhea or heart failure).[citation needed]

Saline is also often used for nasal washes to relieve some of the symptoms of rhinitis and the common cold.[17] The solution exerts a softening and loosening influence on the mucus to make it easier to wash out and clear the nasal passages for both babies[18] and adults.[19] In very rare instances, fatal infection by the amoeba Naegleria fowleri can occur if it enters the body through the nose; therefore tap water must not be used for nasal irrigation. Water is only appropriate for this purpose if it is sterile, distilled, boiled, filtered, or disinfected.[20]

Sterile isotonic saline is also used to fill breast implants for use in breast augmentation surgery, to correct congenital abnormalities such as tuberous breast deformity, and to correct breast asymmetry.[21][22] Saline breast implants are also used in reconstructive surgery post-mastectomy.

#### Eyes

[edit]

Eye drops are saline-containing drops used on the eye. Depending on the condition being treated, they may contain steroids, antihistamines, sympathomimetics, beta receptor blockers, parasympathomimetics, parasympatholytics, prostaglandins, non-steroidal anti-inflammatory drugs (NSAIDs), antibiotics or topical anesthetics. Eye drops sometimes do not have medications in them and are only lubricating and tear-replacing solutions.

#### Nose

[edit]

Main article: Nasal irrigation

There is tentative evidence that saline nasal irrigation may help with long term cases of rhinosinusitis.[23] Evidence for use in cases of rhinosinusitis of short duration is unclear.[24]

#### Scleral tattooing

[edit]

Saline is used in scleral tattooing, coloring the white part of the human eye.[25]

#### Tattoo lightening

[edit]

Main article: Tattoo removal

Saline is used to lighten tattoos (including microblading tattoos) through the process of osmosis.[26]

## Other concentrations

[edit]

Concentrations lower and higher than normal also exist. High concentrations are used rarely in medicine but frequently in molecular biology.

### Hypertonic saline

[edit]

Vial of 23.4% sodium chloride

Hypertonic saline—7% NaCl solutions are considered mucoactive agents and thus are used to hydrate thick secretions (mucus) in order to make it easier to cough up and out (expectorate). 3% hypertonic saline solutions are also used in critical care settings, acutely increased intracranial pressure, or severe hyponatremia.[27] Inhalation of hypertonic saline has also been shown to help in other respiratory problems, specifically bronchiolitis.[28] Hypertonic saline is currently recommended by the Cystic Fibrosis Foundation as a primary part of a cystic fibrosis treatment regimen.[29]

An 11% solution of xylitol with 0.65% saline stimulates the washing of the nasopharynx and has an effect on the nasal pathogenic bacteria. This has been used in complementary and alternative medicine.[30]

Hypertonic saline may be used in perioperative fluid management protocols to reduce excessive intravenous fluid infusions and lessen pulmonary complications.[31] Hypertonic saline is used in treating hyponatremia and cerebral edema. Rapid correction of hyponatremia via hypertonic saline, or via any saline infusion > 40 mmol/L (Na+ having a valence of 1, 40 mmol/L = 40 mEq/L) greatly increases risk of central pontine myelinolysis (CPM), and so requires constant monitoring of the person's response. Water privation combined with diuretic block does not produce as much risk of CPM as saline administration does; however, it does not correct hyponatremia as rapidly as administration of hypertonic saline does. Due to hypertonicity, administration may result in phlebitis and tissue necrosis. As such, concentrations greater than 3% NaCl should normally be administered via a central venous catheter, also known as a 'central line'. Such hypertonic saline is normally available in two strengths, the former of which is more commonly administered:

* 3% NaCl has 513 mEq/L of Na and Cl.
* 5% NaCl has 856 mEq/L of Na and Cl.

Hypertonic NaCl solutions that are less commonly used are 7% (1200 mEq/L) and 23.4% (approx 4000 mEq/L), both of which are used (also via central line), often in conjunction with supplementary diuretics, in the treatment of traumatic brain injury.[32]

### Less concentrated solutions

[edit]

Other concentrations commonly used include:

* Half-normal saline (0.45% NaCl), often with "D5" (5% dextrose), contains 77 mEq/L of Na and Cl and 50 g/L dextrose.
* Quarter-normal saline (0.22% NaCl) has 39 mEq/L of Na and Cl and almost always contains 5% dextrose for osmolality reasons. It can be used alone in neonatal intensive care units.
* Dextrose (glucose) 4% in 0.18% saline is used sometimes for maintenance replacement.

## Solutions with added ingredients

[edit]

In medicine, common types of salines include:

* Ringer's lactate solution
* Acetated Ringer's solution
* Oral rehydration solution
* Intravenous sugar solutions
* 5% dextrose in normal saline (D5NS)
* 10% dextrose in normal saline (D10NS)
* 5% dextrose in half-normal saline (D5HNS)
* 10% dextrose in half-normal saline (D10HNS)

And in cell biology, in addition to the above the following are used:

* Phosphate buffered saline (PBS) (recipes from Dulbecco = D-PBS, Galfre, Kuchler, Ausubel etc.)
* TRIS-buffered saline (TBS) (recipes from Goldsmith, Ausubel etc.)
* Hank's balanced salt solution (HBSS)
* Earle's balanced salt solution (EBSS)
* Standard saline citrate (SSC)
* HEPES-buffered saline (HBS) (recipes from Dittmar, Liu, Ausubel etc.)
* Gey's balanced salt solution (GBSS)

## History

[edit]

Saline was believed to have originated during the Indian Blue cholera pandemic that swept across Europe in 1831. William Brooke O'Shaughnessy, a recent graduate of Edinburgh Medical School, proposed in an article to medical journal The Lancet to inject people infected with cholera with highly oxygenated salts to treat the "universal stagnation of the venous system and rapid cessation of arterialisation of the blood" seen in people with severely dehydrated cholera.[33] He found his treatment harmless in dogs, and his proposal was soon adopted by the physician Thomas Latta in treating people with cholera to beneficial effect. In the following decades, variations and alternatives to Latta's solution were tested and used in treating people with cholera. These solutions contained a range of concentrations of sodium, chloride, potassium, carbonate, phosphate, and hydroxide.

The breakthrough in achieving physiological concentrations was accomplished by Sydney Ringer in the early 1880s,[34] when he determined the optimal salt concentrations to maintain the contractility of frog heart muscle tissue. Normal saline is considered a descendant of the pre-Ringer solutions, as Ringer's findings were not adopted and widely used until decades later. The term "normal saline" itself appears to have little historical basis, except for studies done in 1882–83 by Dutch physiologist Hartog Jacob Hamburger; these in vitro studies of red cell lysis suggested incorrectly that 0.9% was the concentration of salt in human blood (rather than 0.6%, the true concentration).[35]

Normal saline has become widely used in modern medicine, but due to the mismatch with real blood, other solutions have proved better. The 2018 publication of a randomized, controlled trial with 15,000 people in intensive care units showed that compared to normal saline, lactated Ringer's solution reduced the combined risk of mortality, need for additional dialysis, or persistent kidney problems from 15% to 14%, which given the large number of patients is a significant reduction.[36]

## Society and culture

[edit]

Coconut water has been used in place of normal saline in areas without access to normal saline.[37] Its use, however, has not been well studied.[37]

## References

[edit]

1. ^ a b c d "Sodium Chloride Injection - FDA prescribing information, side effects and uses". www.drugs.com. Archived from the original on 18 January 2017. Retrieved 14 January 2017.
2. ^ a b c d British national formulary : BNF 69 (69th ed.). British Medical Association. 2015. pp. 683, 770. ISBN 9780857111562.
3. ^ Marini JJ, Wheeler AP (2010). Critical Care Medicine: The Essentials. Lippincott Williams & Wilkins. p. 54\. ISBN 9780781798396. Archived from the original on 18 September 2017.
4. ^ "Hypertonic Saline - FDA prescribing information, side effects and uses". www.drugs.com. Archived from the original on 18 January 2017. Retrieved 14 January 2017.
5. ^ Pestana C (2000). Fluids and Electrolytes in the Surgical Patient. Lippincott Williams & Wilkins. p. 11\. ISBN 9780781724258. Archived from the original on 18 September 2017.
6. ^ Reddi BA (2013). "Why is saline so acidic (and does it really matter?)". International Journal of Medical Sciences. 10 (6): 747–750. doi:10.7150/ijms.5868. PMC 3638298. PMID 23630439.
7. ^ Bozzetti F, Staun M, van Gossum A (2014). Home Parenteral Nutrition (2nd ed.). CABI. p. 4\. ISBN 9781780643113. Archived from the original on 18 September 2017.
8. ^ World Health Organization (2019). World Health Organization model list of essential medicines: 21st list 2019. Geneva: World Health Organization. hdl:10665/325771. WHO/MVP/EMP/IAU/2019.06. License: CC BY-NC-SA 3.0 IGO.
9. ^ "The Top 300 of 2022". ClinCalc. Archived from the original on 30 August 2024. Retrieved 30 August 2024.
10. ^ "Sodium Salts Drug Usage Statistics, United States, 2013 - 2022". ClinCalc. Retrieved 30 August 2024.
11. ^ Prough DS, Bidani A (May 1999). "Hyperchloremic metabolic acidosis is a predictable consequence of intraoperative infusion of 0.9% saline". Anesthesiology. 90 (5): 1247–1249. doi:10.1097/00000542-199905000-00003. PMID 10319767.
12. ^ "Fluid Density Calculator". Earthwardconsulting.com. Archived from the original on 16 September 2009. Retrieved 27 February 2011.
13. ^ "Water Density Calculator". Csgnetwork.com. Archived from the original on 22 January 2010. Retrieved 27 February 2011.
14. ^ Lote CJ. Principles of Renal Physiology, 5th edition. Springer. p. 6.
15. ^ Hamer WJ, Wu YC (1 October 1972). "Osmotic Coefficients and Mean Activity Coefficients of Uni-univalent Electrolytes in Water at 25°C". Journal of Physical and Chemical Reference Data. 1 (4): 1047–1100. Bibcode:1972JPCRD...1.1047H. doi:10.1063/1.3253108.
16. ^ Brown A (20 August 2018). Ford S (ed.). "When is wound cleansing necessary and what solution should be used?". Nursing Times. Vol. 114, no. 9\. Metropolis International. pp. 42–45. Retrieved 26 December 2020.
17. ^ "Cure a cold: Saline Nasal drops". Archived from the original on 16 January 2013.
18. ^ "Blocked Nose in Babies ('Snuffles')". Patient UK.
19. ^ "Tixylix saline nasal drops". Netdoctor. 30 March 2011. Archived from the original on 1 November 2012.
20. ^ "Sinus Rinsing For Health or Religious Practice". CDC. 28 February 2017.
21. ^ Eisenberg T (October 2021). "Does Overfilling Smooth Inflatable Saline-Filled Breast Implants Decrease the Deflation Rate? Experience with 4761 Augmentation Mammaplasty Patients". Aesthetic Plastic Surgery. 45 (5): 1991–1999. doi:10.1007/s00266-021-02198-3. PMC 8481168. PMID 33712871.
22. ^ Eisenberg T (2019). "One-Stage Correction of Tuberous Breast Deformity Using Saline Implants: Without the Need for Radial Scoring or Lowering the Inframammary Fold". American Journal of Cosmetic Surgery. 36 (4): 191–196. doi:10.1177/0748806819841466. S2CID 145932734.
23. ^ Succar EF, Turner JH, Chandra RK (May 2019). "Nasal saline irrigation: a clinical update". International Forum of Allergy & Rhinology. 9 (S1): S4–S8. doi:10.1002/alr.22330. PMID 31087631.
24. ^ Achilles N, Mösges R (April 2013). "Nasal saline irrigations for the symptoms of acute and chronic rhinosinusitis". Current Allergy and Asthma Reports. 13 (2): 229–235. doi:10.1007/s11882-013-0339-y. PMID 23354530. S2CID 9798807.
25. ^ "A Model Almost Lost Her Eye After Getting a Sclera Tattoo. Here's Why She Did It". TIME. 29 September 2017. Retrieved 28 April 2024. "It happens when a mixture of ink and saline is injected into the eye through a small needle."
26. ^ Dall'Asen N (18 February 2022). "OK, I'll Say It: I Regret Getting My Eyebrows Microbladed". Allure. Retrieved 28 April 2024.
27. ^ Reeves EP, Williamson M, O'Neill SJ, Greally P, McElvaney NG (June 2011). "Nebulized hypertonic saline decreases IL-8 in sputum of patients with cystic fibrosis". American Journal of Respiratory and Critical Care Medicine. 183 (11): 1517–1523. doi:10.1164/rccm.201101-0072OC. PMID 21330456.
28. ^ Principi T, Komar L (2011). "A critical review of "a randomized trial of nebulized 3% hypertonic saline with epinephrine in the treatment of acute bronchiolitis in the emergency department."". Journal of Population Therapeutics and Clinical Pharmacology = Journal de la Therapeutique des Populations et de la Pharmacologie Clinique. 18 (2): e273–e274. PMID 21633141.
29. ^ O'Connell OJ, O'Farrell C, Harrison MJ, Eustace JA, Henry MT, Plant BJ (June 2011). "Nebulized hypertonic saline via positive expiratory pressure versus via jet nebulizer in patients with severe cystic fibrosis". Respiratory Care. 56 (6): 771–775. doi:10.4187/respcare.00866. PMID 21333079. S2CID 26080152.
30. ^ Jones A. "Intranasal Xylitol, Recurrent Otitis Media, and Asthma: Report of Three Cases*". Nasal xylitol, from Clinical Practice of Alternative Medicine. Alonzo H. Jones, DO. Archived from the original on 8 May 2014. Retrieved 7 May 2014.
31. ^ Shrum B, Church B, McArthur E, Burns KE, Znajda T, McAlister V (June 2016). "Hypertonic salt solution for peri-operative fluid management". The Cochrane Database of Systematic Reviews. 2016 (6): CD005576. doi:10.1002/14651858.CD005576.pub3. PMC 8627702. PMID 27271480.
32. ^ Mortimer DS, Jancik J (June 2006). "Administering hypertonic saline to patients with severe traumatic brain injury". The Journal of Neuroscience Nursing. 38 (3): 142–6. doi:10.1097/01376517-200606000-00002. PMID 16817665. Archived from the original on 19 November 2010 - via Medscape.
33. ^ O'Shaugnessy WB (1831). "Proposal for a new method of treating the blue epidemic cholera by the injection of highly-oxygenated salts into the venous system". Lancet. 17 (432): 366–71. doi:10.1016/S0140-6736(02)94163-2.
34. ^ Sutin KM, Marino PL (2007). The ICU book. Lippincott Williams & Wilkins. ISBN 978-0-7817-4802-5.
35. ^ Awad S, Allison SP, Lobo DN (April 2008). "The history of 0.9% saline". Clinical Nutrition. 27 (2): 179–188. doi:10.1016/j.clnu.2008.01.008. PMID 18313809.
36. ^ Dalton C (31 March 2018). "Why Did Sterile Salt Water Become The IV Fluid Of Choice?". NPR.org.
37. ^ a b Campbell-Falck D, Thomas T, Falck TM, Tutuo N, Clem K (January 2000). "The intravenous use of coconut water". The American Journal of Emergency Medicine. 18 (1): 108–111. doi:10.1016/s0735-6757(00)90062-7. PMID 10674546.

* v
* t
* e

Intravenous therapy

Infused substances|

| Blood-based product|

* Whole blood
* PF24/Fresh frozen plasma (Cryosupernatant \+ Cryoprecipitate)

|

Blood substitute|

* Hemoglobin-based

Volume expander|

* Ringer's lactate
* Normal saline
* Sugar solution

Buffers|

* Ringer's lactate
* Sodium bicarbonate

Medications|

* Immunoglobulin therapy
* Gamma globulin
* Interferon

Parenteral nutrition|

* Sugar

Other|

* Drug injection
* Ascorbic acid

Access points|

* Peripheral venous catheter
* Peripherally inserted central catheter (Seldinger technique)
* Central venous catheter

Other equipment|

* Infusion pump
* CVC
* SLC
* MLC
* PICC
* Implantable catheter
* Port
* Thermodilution catheter
* Dialysis catheter

Specific risks|

* Air embolism
* Extravasation

See also|

* Needle and syringe programmes
* Supervised injection site

Portal:

* Medicine

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