succinate—CoA ligase (GDP-forming)

Pig GTP-specific succinyl-CoA synthetase with GTP. PDB 2fp4 [1]

Identifiers
EC no.| 6.2.1.4
CAS no.| 9014-36-2
Databases
IntEnz| IntEnz view
BRENDA| BRENDA entry
ExPASy| NiceZyme view
KEGG| KEGG entry
MetaCyc| metabolic pathway
PRIAM| profile
PDB structures| RCSB PDB PDBe PDBsum
Gene Ontology| AmiGO  / QuickGO
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PMC| articles
PubMed| articles
NCBI| proteins

In enzymology, a succinate—CoA ligase (GDP-forming) (EC 6.2.1.4) is an enzyme that catalyzes the chemical reaction

GTP + succinate + CoA  ⇌ {\displaystyle \rightleftharpoons } GDP + phosphate + succinyl-CoA

The 3 substrates of this enzyme are GTP, succinate, and CoA, whereas its 3 products are GDP, phosphate, and succinyl-CoA.

This enzyme belongs to the family of ligases, specifically those forming carbon-sulfur bonds as acid-thiol ligases. The systematic name of this enzyme class is succinate:CoA ligase (GDP-forming). Other names in common use include succinyl-CoA synthetase (GDP-forming), succinyl coenzyme A synthetase (guanosine diphosphate-forming), succinate thiokinase, succinic thiokinase, succinyl coenzyme A synthetase, succinate-phosphorylating enzyme, P-enzyme, SCS, G-STK, succinyl coenzyme A synthetase (GDP-forming), succinyl CoA synthetase, and succinyl coenzyme A synthetase. This enzyme participates in the citric acid cycle and propanoate metabolism.

## Structural studies

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As of late 2007, 6 structures have been solved for this class of enzymes, with PDB accession codes 1EUC, 1EUD, 2FP4, 2FPG, 2FPI, and 2FPP.

## References

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1. ^ Fraser, M. E.; Hayakawa, K.; Hume, M. S.; Ryan, D. G.; Brownie, E. R. (2006). "Interactions of GTP with the ATP-grasp Domain of GTP-specific Succinyl-CoA Synthetase". Journal of Biological Chemistry. 281 (16): 11058–11065\. doi:10.1074/jbc.M511785200. PMID 16481318.

* Boyer, P.D., Lardy, H. and Myrback, K. (Eds.), The Enzymes, 2nd ed., vol. 6, Academic Press, New York, 1962, p. 387-399.
* Kaufman S, Gilvarg C, Cori O, Ochoa S (1953). "Enzymatic oxidation of alpha-ketoglutarate and coupled phosphorylation". J. Biol. Chem. 203 (2): 869–888\. doi:10.1016/S0021-9258(19)52356-0. PMID 13084656.
* Mazumder R, Sanadi DR, Rodwell WV (1960). "Purification and properties of hog kidney succinic thiokinase". J. Biol. Chem. 235 (9): 2546–2550\. doi:10.1016/S0021-9258(19)76911-7. PMID 13768680.
* SANADI DR, GIBSON M, AYENGAR P (1954). "Guanosine triphosphate, the primary product of phosphorylation coupled to the breakdown of succinyl coenzyme A". Biochim. Biophys. Acta. 14 (3): 434–6\. doi:10.1016/0006-3002(54)90205-X. PMID 13181903.

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Enzymes: CO CS and CN ligases (EC 6.1-6.3)

6.1: Carbon-Oxygen|

* Aminoacyl tRNA synthetase
* Alanine
* Arginine
* Asparagine
* Aspartate
* Cysteine
* D-Alanine—poly(phosphoribitol) ligase
* Glutamate
* Glutamine
* Glycine
* Histidine
* Isoleucine
* Leucine
* Lysine
* Methionine
* Phenylalanine
* Proline
* Serine
* Threonine
* Tryptophan
* Tyrosine
* Valine

6.2: Carbon-Sulfur|

* (2,2,3-trimethyl-5-oxocyclopent-3-enyl)acetyl-CoA synthase
* 2-furoate—CoA ligase
* 3-alpha,7-alpha-dihydroxy-5-beta-cholestanate—CoA ligase
* 3-hydroxybenzoate—CoA ligase
* 3-hydroxypropionyl-CoA synthase
* 4-chlorobenzoate—CoA ligase
* 4-Coumarate-CoA ligase
* 4-hydroxybenzoate—CoA ligase
* 6-carboxyhexanoate—CoA ligase
* Acetate—ACP ligase
* Acetate—CoA ligase (ADP-forming)
* Acetoacetate—CoA ligase
* Acetyl—CoA synthetase
* Acid—CoA ligase (GDP-forming)
* Anthranilate—CoA ligase
* Arachidonate—CoA ligase
* Benzoate—CoA ligase
* Biotin—CoA ligase
* (butirosin acyl-carrier protein)—L-glutamate ligase
* Butyrate—CoA ligase
* Cholate—CoA ligase
* Citrate—CoA ligase
* (citrate (pro-3S)-lyase) ligase
* Dicarboxylate—CoA ligase
* Glutarate—CoA ligase
* Long-chain-fatty-acid—ACP ligase
* Long-chain-fatty-acid—CoA ligase
* Malate—CoA ligase
* Malonate—CoA ligase
* o-Succinylbenzoate—CoA ligase
* Oxalate—CoA ligase
* Phenylacetate—CoA ligase
* Phytanate—CoA ligase
* Propionate—CoA ligase
* Succinate—CoA ligase (ADP-forming)
* Succinate—CoA ligase (GDP-forming)
* Succinyl—CoA synthetase
* trans-Feruloyl—CoA synthase

6.3: Carbon-Nitrogen|

* Glutamine synthetase
* Ubiquitin ligase
* Cullin
* Von Hippel–Lindau tumor suppressor
* UBE3A
* Mdm2
* Anaphase-promoting complex
* UBR1
* Glutathione synthetase
* CTP synthetase
* Adenylosuccinate synthase
* Argininosuccinate synthase
* Holocarboxylase synthetase
* GMP synthase
* Asparagine synthetase
* Carbamoyl phosphate synthetase
* I
* II
* Glutamate–cysteine ligase

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Enzymes

Activity|

* Active site
* Binding site
* Catalytic triad
* Oxyanion hole
* Enzyme promiscuity
* Diffusion-limited enzyme
* Cofactor
* Enzyme catalysis

Regulation|

* Allosteric regulation
* Cooperativity
* Enzyme inhibitor
* Enzyme activator

Classification|

* EC number
* Enzyme superfamily
* Enzyme family
* List of enzymes

Kinetics|

* Enzyme kinetics
* Eadie–Hofstee diagram
* Hanes–Woolf plot
* Lineweaver–Burk plot
* Michaelis–Menten kinetics

Types|

* EC1 Oxidoreductases (list)
* EC2 Transferases (list)
* EC3 Hydrolases (list)
* EC4 Lyases (list)
* EC5 Isomerases (list)
* EC6 Ligases (list)
* EC7 Translocases (list)

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