Thiazole synthase

Thiazole synthase oktamer, Saccharomyces cerevisiae

Identifiers
EC no.| 2.8.1.10
Databases
IntEnz| IntEnz view
BRENDA| BRENDA entry
ExPASy| NiceZyme view
KEGG| KEGG entry
MetaCyc| metabolic pathway
PRIAM| profile
PDB structures| RCSB PDB PDBe PDBsum
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PMC| articles
PubMed| articles
NCBI| proteins

Thiazole synthase (EC 2.8.1.10, thiG (gene)) is an enzyme with systematic name 1-deoxy-D-xylulose 5-phosphate:thiol sulfurtransferase.[1][2][3][4][5][6] This enzyme catalyses the following chemical reaction

1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-adenylate-[sulfur-carrier protein ThiS]  ⇌ {\displaystyle \rightleftharpoons } 2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O

H2S can provide the sulfur in vitro.

## References[edit]

1. ^ Park JH, Dorrestein PC, Zhai H, Kinsland C, McLafferty FW, Begley TP (October 2003). "Biosynthesis of the thiazole moiety of thiamin pyrophosphate (vitamin B1)". Biochemistry. 42 (42): 12430–8. doi:10.1021/bi034902z. PMID 14567704.
2. ^ Dorrestein PC, Zhai H, McLafferty FW, Begley TP (October 2004). "The biosynthesis of the thiazole phosphate moiety of thiamin: the sulfur transfer mediated by the sulfur carrier protein ThiS". Chemistry & Biology. 11 (10): 1373–81. doi:10.1016/j.chembiol.2004.08.009. PMID 15489164.
3. ^ Dorrestein PC, Zhai H, Taylor SV, McLafferty FW, Begley TP (March 2004). "The biosynthesis of the thiazole phosphate moiety of thiamin (vitamin B1): the early steps catalyzed by thiazole synthase". Journal of the American Chemical Society. 126 (10): 3091–6. doi:10.1021/ja039616p. PMID 15012138.
4. ^ Settembre EC, Dorrestein PC, Zhai H, Chatterjee A, McLafferty FW, Begley TP, Ealick SE (September 2004). "Thiamin biosynthesis in Bacillus subtilis: structure of the thiazole synthase/sulfur carrier protein complex". Biochemistry. 43 (37): 11647–57. doi:10.1021/bi0488911. PMID 15362849.
5. ^ Hazra A, Chatterjee A, Begley TP (March 2009). "Biosynthesis of the thiamin thiazole in Bacillus subtilis: identification of the product of the thiazole synthase-catalyzed reaction". Journal of the American Chemical Society. 131 (9): 3225–9. doi:10.1021/ja806752h. PMC 2765510. PMID 19216519.
6. ^ Hazra AB, Han Y, Chatterjee A, Zhang Y, Lai RY, Ealick SE, Begley TP (June 2011). "A missing enzyme in thiamin thiazole biosynthesis: identification of TenI as a thiazole tautomerase". Journal of the American Chemical Society. 133 (24): 9311–9. doi:10.1021/ja1110514. PMC 3116082. PMID 21534620.

## External links[edit]

* Thiazole+synthase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)

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Transferases: sulfur-containing group (EC 2.8)

2.8.1: Sulfurtransferases|

* Thiosulfate sulfurtransferase
* Rhodanese
* 3-mercaptopyruvate sulfurtransferase
* thiosulfate—thiol sulfurtransferase
* tRNA sulfurtransferase
* thiosulfate—dithiol sulfurtransferase
* biotin synthase
* cysteine desulfurase

2.8.2: Sulfotransferases|

* Alcohol sulfotransferase
* Tyrosylprotein sulfotransferase
* Aryl sulfotransferase

2.8.3: CoA-transferases|

* Propionate CoA-transferase

2.8.4: Alkylthio|

* Coenzyme-B sulfoethylthiotransferase

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Enzymes

Activity|

* Active site
* Binding site
* Catalytic triad
* Oxyanion hole
* Enzyme promiscuity
* Diffusion-limited enzyme
* Coenzyme
* Cofactor
* Enzyme catalysis

Regulation|

* Allosteric regulation
* Cooperativity
* Enzyme inhibitor
* Enzyme activator

Classification|

* EC number
* Enzyme superfamily
* Enzyme family
* List of enzymes

Kinetics|

* Enzyme kinetics
* Eadie–Hofstee diagram
* Hanes–Woolf plot
* Lineweaver–Burk plot
* Michaelis–Menten kinetics

Types|

* EC1 Oxidoreductases (list)
* EC2 Transferases (list)
* EC3 Hydrolases (list)
* EC4 Lyases (list)
* EC5 Isomerases (list)
* EC6 Ligases (list)
* EC7 Translocases (list)

Portal:

* Biology

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