Trans-splicing

Maturation process joining exons from different pre-mRNAs into a mature mRNA

Trans-splicing is a special form of RNA processing where exons from two different primary RNA transcripts are joined end to end and ligated. It is usually found in eukaryotes and mediated by the spliceosome, although some bacteria and archaea also have "half-genes" for tRNAs.[1]

## Genic trans-splicing

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Whereas "normal" (cis-)splicing processes a single molecule, trans-splicing generates a single RNA transcript from multiple separate pre-mRNAs. This phenomenon can be exploited for molecular therapy to address mutated gene products.[2] Genic trans-splicing allows variability in RNA diversity and increases proteome complexity.[3]

### Oncogenesis

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While some fusion transcripts occur via trans-splicing in normal human cells,[1] trans-splicing can also be the mechanism behind certain oncogenic fusion transcripts.[4][5]

## SL trans-splicing

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Spliced leader (SL) trans-splicing is used by certain microorganisms, notably protists of the Kinetoplastea class to express genes. In these organisms, a capped splice leader RNA is transcribed, and simultaneously, genes are transcribed in long polycistrons.[6] The capped splice leader is trans-spliced onto each gene to generate monocistronic capped and polyadenylated transcripts.[7] These early-diverging eukaryotes use few introns, and the spliceosome they possess show some unusual variations in their structure assembly.[7][8] They also possess multiple eIF4E isoforms with specialized roles in capping.[9] The spliced leader sequence is highly conserved in lower species that undergo trans-splicing. Such as trypanosomes. While the spliced leader's role is not known in the cell, it's thought to be involved in translation initiation. In C.elegans, the splicing of the sequence leader occurs close to the initiation codon. Some scientists also suggest the sequence is required for cell viability. In Ascaris, the spliced leader sequence is needed to the RNA gene can be transcribed. The Spliced leader sequence may be responsible for initiation, mRNA localization, and translation initiation or inhibition.[10]

Some other eukaryotes, notably among dinoflagellates, sponges, nematodes, cnidarians, ctenophores, flatworms, crustaceans, chaetognaths, rotifers, and tunicates also use more or less frequently the SL trans-splicing.[1][11] In the tunicate Ciona intestinalis, the extent of SL trans-splicing is better described by a quantitative view recognising frequently and infrequently trans-spliced genes rather than a binary and conventional categorisation of trans-spliced versus non-trans-spliced genes.[12]

The SL trans-splicing functions in the resolution of polycistronic transcripts of operons into individual 5'-capped mRNAs. This processing is achieved when the outrons are trans-spliced to unpaired, downstream acceptor sites adjacent to cistron open reading frames.[13][14]

## Mechanism

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Trans-splicing is characterized by the joining of two separate exons transcribed RNAs. The signal for this splicing is the outron at the 5’ end of the mRNA, in the absence of a functional 5’ splice site upstream. When the 5’ outron in spliced, the 5’ splice site of the spliced leader RNA is branched to the outron and forms an intermediate.[10] This step results in a free spliced leader exon. The exon is then spliced to the first exon on the pre-mRNA and the intermediate is released. Trans-splicing differs from cis-splicing in that there is no 5' splice site on the pre-mRNA. Instead the 5' splice site is provided by the SL sequence.[14]

## Trans-splicing between sense and anti-sense strands

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As a result of the sense strand undergoing transcription, a pre-mRNA is formed that complements the sense strand. The anti-sense strand is also transcribed resulting in a complementary pre-mRNA strand. The exons from the two transcripts are spliced together to form a chimeric mRNA.[15]

## Alternative Trans-splicing

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Alternative trans-splicing includes intragenic trans-splicing and intergenic trans-splicing. Intragenic trans-splicing involves duplication of exons in the pre-mRNA. Intergenic trans-splicing is characterized by the splicing together of exons formed form the pre-mRNA of two different genes, resulting in trans-genic mRNA.[16]

## See also

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* Chimera (EST)

## References

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1. ^ a b c Lei Q, Li C, Zuo Z, Huang C, Cheng H, Zhou R (March 2016). "Evolutionary Insights into RNA trans-Splicing in Vertebrates". Genome Biology and Evolution. 8 (3): 562–77\. doi:10.1093/gbe/evw025. PMC 4824033. PMID 26966239.
2. ^ Iwasaki R, Kiuchi H, Ihara M, Mori T, Kawakami M, Ueda H (July 2009). "Trans-splicing as a novel method to rapidly produce antibody fusion proteins". Biochemical and Biophysical Research Communications. 384 (3): 316–21\. Bibcode:2009BBRC..384..316I. doi:10.1016/j.bbrc.2009.04.122. PMID 19409879.
3. ^ Lasda, Erika L.; Blumenthal, Thomas (2011). "Trans-splicing". WIREs RNA. 2 (3): 417–434\. doi:10.1002/wrna.71. ISSN 1757-7012. PMID 21957027. S2CID 209567118.
4. ^ Li H, Wang J, Mor G, Sklar J (September 2008). "A neoplastic gene fusion mimics trans-splicing of RNAs in normal human cells". Science. 321 (5894): 1357–61\. Bibcode:2008Sci...321.1357L. doi:10.1126/science.1156725. PMID 18772439. S2CID 13605087.
5. ^ Rickman DS, Pflueger D, Moss B, VanDoren VE, Chen CX, de la Taille A, et al. (April 2009). "SLC45A3-ELK4 is a novel and frequent erythroblast transformation-specific fusion transcript in prostate cancer". Cancer Research. 69 (7): 2734–8\. doi:10.1158/0008-5472.CAN-08-4926. PMC 4063441. PMID 19293179.
6. ^ Campbell DA, Sturm NR, Yu MC (February 2000). "Transcription of the kinetoplastid spliced leader RNA gene". Parasitology Today. 16 (2): 78–82\. doi:10.1016/s0169-4758(99)01545-8. PMID 10652494.
7. ^ a b Liang XH, Haritan A, Uliel S, Michaeli S (October 2003). "trans and cis splicing in trypanosomatids: mechanism, factors, and regulation". Eukaryotic Cell. 2 (5): 830–40\. doi:10.1128/EC.2.5.830-840.2003. PMC 219355. PMID 14555465.
8. ^ Günzl A (August 2010). "The pre-mRNA splicing machinery of trypanosomes: complex or simplified?". Eukaryotic Cell. 9 (8): 1159–70\. doi:10.1128/EC.00113-10. PMC 2918933. PMID 20581293.
9. ^ Freire ER, Sturm NR, Campbell DA, de Melo Neto OP (October 2017). "The Role of Cytoplasmic mRNA Cap-Binding Protein Complexes in Trypanosoma brucei and Other Trypanosomatids". Pathogens. 6 (4): 55. doi:10.3390/pathogens6040055. PMC 5750579. PMID 29077018.
10. ^ a b Girard, Lisa R.; Fiedler, Tristan J.; Harris, Todd W.; Carvalho, Felicia; Antoshechkin, Igor; Han, Michael; Sternberg, Paul W.; Stein, Lincoln D.; Chalfie, Martin (January 2007). "WormBook: the online review of Caenorhabditis elegans biology". Nucleic Acids Research. 35 (Database issue): D472–475. doi:10.1093/nar/gkl894. ISSN 1362-4962. PMC 1669767. PMID 17099225.
11. ^ Lasda EL, Blumenthal T (2011-05-01). "Trans-splicing". Wiley Interdisciplinary Reviews: RNA. 2 (3): 417–34\. doi:10.1002/wrna.71. PMID 21957027. S2CID 209567118.
12. ^ Matsumoto J, Dewar K, Wasserscheid J, Wiley GB, Macmil SL, Roe BA, et al. (May 2010). "High-throughput sequence analysis of Ciona intestinalis SL trans-spliced mRNAs: alternative expression modes and gene function correlates". Genome Research. 20 (5): 636–45\. doi:10.1101/gr.100271.109. PMC 2860165. PMID 20212022.
13. ^ Clayton, Christine E. (2002-04-15). "Life without transcriptional control? From fly to man and back again". The EMBO Journal. 21 (8): 1881–1888\. doi:10.1093/emboj/21.8.1881. ISSN 1460-2075. PMC 125970. PMID 11953307.
14. ^ a b Blumenthal, Thomas; Gleason, Kathy Seggerson (February 2003). "Caenorhabditis elegans operons: form and function". Nature Reviews Genetics. 4 (2): 110–118\. doi:10.1038/nrg995. ISSN 1471-0056. PMID 12560808. S2CID 9864778.
15. ^ Lei, Quan; Li, Cong; Zuo, Zhixiang; Huang, Chunhua; Cheng, Hanhua; Zhou, Rongjia (March 2016). "Evolutionary Insights into RNA trans-Splicing in Vertebrates". Genome Biology and Evolution. 8 (3): 562–577\. doi:10.1093/gbe/evw025. ISSN 1759-6653. PMC 4824033. PMID 26966239.
16. ^ Horiuchi, Takayuki; Aigaki, Toshiro (February 2006). "Alternative trans-splicing: a novel mode of pre-mRNA processing". Biology of the Cell. 98 (2): 135–140\. doi:10.1042/bc20050002. ISSN 0248-4900. PMID 16417469. S2CID 10335534.

## Further reading

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* Dixon RJ, Eperon IC, Samani NJ (January 2007). "Complementary intron sequence motifs associated with human exon repetition: a role for intragenic, inter-transcript interactions in gene expression". Bioinformatics. 23 (2): 150–5\. doi:10.1093/bioinformatics/btl575. PMID 17105720.
* Yang Y, Walsh CE (December 2005). "Spliceosome-mediated RNA trans-splicing". Molecular Therapy. 12 (6): 1006–12\. doi:10.1016/j.ymthe.2005.09.006. PMID 16226059.
* Coady TH, Shababi M, Tullis GE, Lorson CL (August 2007). "Restoration of SMN function: delivery of a trans-splicing RNA re-directs SMN2 pre-mRNA splicing". Molecular Therapy. 15 (8): 1471–8\. doi:10.1038/sj.mt.6300222. PMID 17551501.
* Wally V, Murauer EM, Bauer JW (August 2012). "Spliceosome-mediated trans-splicing: the therapeutic cut and paste". The Journal of Investigative Dermatology. 132 (8): 1959–66\. doi:10.1038/jid.2012.101. PMID 22495179.