Vesicle-associated membrane protein 8

Protein-coding gene in the species Homo sapiens

VAMP8

| Available structures

PDB| Ortholog search: PDBe RCSB
| List of PDB id codes

4WY4

Identifiers
Aliases| VAMP8, EDB, VAMP-8, vesicle associated membrane protein 8
External IDs| OMIM: 603177; MGI: 1336882; HomoloGene: 37846; GeneCards: VAMP8; OMA:VAMP8 - orthologs
| Gene location (Human)

Chr.| Chromosome 2 (human)[1]

Band| 2p11.2| Start| 85,561,562 bp[1]
End| 85,582,031 bp[1]
| Gene location (Mouse)

Chr.| Chromosome 6 (mouse)[2]

Band| 6 C1|6 32.27 cM| Start| 72,362,206 bp[2]
End| 72,367,686 bp[2]
| RNA expression pattern

Bgee| | Human| Mouse (ortholog)

|

| Top expressed in

* palpebral conjunctiva

* monocyte

* mucosa of transverse colon

* olfactory zone of nasal mucosa

* granulocyte

* jejunal mucosa

* rectum

* nasal epithelium

* bronchial epithelial cell

* skin of abdomen

| Top expressed in

* proximal tubule

* right kidney

* yolk sac

* granulocyte

* lung

* bone marrow

* human kidney

* urinary bladder

* duodenum

* lip

More reference expression data
BioGPS| |

More reference expression data
| Gene ontology

Molecular function|

* SNAP receptor activity
* protein binding
* chloride channel inhibitor activity
* SNARE binding

Cellular component|

* cytoplasm
* integral component of membrane
* recycling endosome
* vesicle
* cytosol
* endosome
* early endosome membrane
* membrane
* secretory granule membrane
* late endosome membrane
* plasma membrane
* mucin granule
* lysosomal membrane
* SNARE complex
* early endosome
* perinuclear region of cytoplasm
* lysosome
* extracellular exosome
* clathrin-coated vesicle membrane
* phagocytic vesicle membrane
* azurophil granule membrane
* specific granule membrane
* recycling endosome membrane
* tertiary granule membrane
* synaptic vesicle

Biological process|

* post-Golgi vesicle-mediated transport
* autophagy
* autophagosome maturation
* negative regulation of secretion by cell
* protein transport
* viral entry into host cell
* vesicle fusion
* eosinophil degranulation
* vesicle-mediated transport
* mucus secretion
* regulation of protein localization to plasma membrane
* positive regulation of histamine secretion by mast cell
* antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent
* autophagosome membrane docking
* neutrophil degranulation
* membrane organization
* regulation of molecular function
* transport

Sources:Amigo / QuickGO
| Orthologs

Species| Human| Mouse
Entrez| |

8673

22320

Ensembl| |

ENSG00000118640

ENSMUSG00000050732

UniProt| |

Q9BV40

O70404

RefSeq (mRNA)| |

NM_003761

NM_016794

RefSeq (protein)| |

NP_003752

NP_058074

Location (UCSC)| Chr 2: 85.56 – 85.58 Mb| Chr 6: 72.36 – 72.37 Mb
PubMed search| [3]| [4]
Wikidata
| View/Edit Human| View/Edit Mouse

|

Vesicle-associated membrane protein 8 is a protein that in humans is encoded by the VAMP8 gene.[5][6][7]

Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. The protein encoded by this gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. It is associated with the perinuclear vesicular structures of the early endocytic compartment. It has been found that VAMP8 interacts specifically with the soluble NSF-attachment protein (alpha-SNAP), most likely through an VAMP8-containing SNARE complex.[7] Phosphorylation of VAMP8 inside the conserved SNARE-domain can suppress vesicle fusion.[8]

## Interactions

[edit]

Vesicle-associated membrane protein 8 has been shown to interact with STX4,[9][10] SNAP23,[9][11] STX1A,[12] STX8[13] and STX7.[13][14]

## References

[edit]

1. ^ a b c GRCh38: Ensembl release 89: ENSG00000118640 - Ensembl, May 2017
2. ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000050732 - Ensembl, May 2017
3. ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. ^ Bui TD, Wong SH, Lu L, Hong W (February 1999). "Endobrevin maps to chromosome 2 in human and chromosome 6 in mouse". Genomics. 54 (3): 579–80. doi:10.1006/geno.1998.5596. PMID 9878266.
6. ^ Wong SH, Zhang T, Xu Y, Subramaniam VN, Griffiths G, Hong W (July 1998). "Endobrevin, a Novel Synaptobrevin/VAMP-Like Protein Preferentially Associated with the Early Endosome". Mol Biol Cell. 9 (6): 1549–63. doi:10.1091/mbc.9.6.1549. PMC 25382. PMID 9614193.
7. ^ a b "Entrez Gene: VAMP8 vesicle-associated membrane protein 8 (endobrevin)".
8. ^ Malmersjö S, Di Palma S, Diao J, Lai Y, Pfuetzner RA, Wang AL, McMahon MA, Hayer A, Porteus M, Bodenmiller B, Brunger AT, Meyer T (July 2016). "Phosphorylation of residues inside the SNARE complex suppresses secretory vesicle fusion". EMBO Journal. 35 (16): 1721–1843. doi:10.15252/embj.201694071. PMC 5010044. PMID 27402227.
9. ^ a b Paumet F, Le Mao J, Martin S, Galli T, David B, Blank U, Roa M (June 2000). "Soluble NSF attachment protein receptors (SNAREs) in RBL-2H3 mast cells: functional role of syntaxin 4 in exocytosis and identification of a vesicle-associated membrane protein 8-containing secretory compartment". J. Immunol. 164 (11): 5850–7. doi:10.4049/jimmunol.164.11.5850. ISSN 0022-1767. PMID 10820264.
10. ^ Polgár J, Chung Sul-Hee, Reed Guy L (August 2002). "Vesicle-associated membrane protein 3 (VAMP-3) and VAMP-8 are present in human platelets and are required for granule secretion". Blood. 100 (3): 1081–3. doi:10.1182/blood.V100.3.1081. ISSN 0006-4971. PMID 12130530. S2CID 36597939.
11. ^ Imai A, Nashida Tomoko, Yoshie Sumio, Shimomura Hiromi (August 2003). "Intracellular localisation of SNARE proteins in rat parotid acinar cells: SNARE complexes on the apical plasma membrane". Arch. Oral Biol. 48 (8): 597–604. doi:10.1016/S0003-9969(03)00116-X. ISSN 0003-9969. PMID 12828989.
12. ^ Nagamatsu S, Nakamichi Y, Watanabe T, Matsushima S, Yamaguchi S, Ni J, Itagaki E, Ishida H (January 2001). "Localization of cellubrevin-related peptide, endobrevin, in the early endosome in pancreatic beta cells and its physiological function in exo-endocytosis of secretory granules". J. Cell Sci. 114 (Pt 1): 219–227. doi:10.1242/jcs.114.1.219. ISSN 0021-9533. PMID 11112705.
13. ^ a b Antonin W, Holroyd C, Fasshauer D, Pabst S, Von Mollard G F, Jahn R (Dec 2000). "A SNARE complex mediating fusion of late endosomes defines conserved properties of SNARE structure and function". EMBO J. 19 (23): 6453–64. doi:10.1093/emboj/19.23.6453. ISSN 0261-4189. PMC 305878. PMID 11101518.
14. ^ Wade N, Bryant N J, Connolly L M, Simpson R J, Luzio J P, Piper R C, James D E (June 2001). "Syntaxin 7 complexes with mouse Vps10p tail interactor 1b, syntaxin 6, vesicle-associated membrane protein (VAMP)8, and VAMP7 in b16 melanoma cells". J. Biol. Chem. 276 (23): 19820–7. doi:10.1074/jbc.M010838200. ISSN 0021-9258. PMID 11278762.

## Further reading

[edit]

* Bonaldo MF, Lennon G, Soares MB (1997). "Normalization and subtraction: two approaches to facilitate gene discovery". Genome Res. 6 (9): 791–806. doi:10.1101/gr.6.9.791. PMID 8889548.
* Fasshauer D, Antonin W, Margittai M, et al. (1999). "Mixed and non-cognate SNARE complexes. Characterization of assembly and biophysical properties". J. Biol. Chem. 274 (22): 15440–6. doi:10.1074/jbc.274.22.15440. PMID 10336434.
* Kim PK, Hollerbach C, Trimble WS, et al. (2000). "Identification of the endoplasmic reticulum targeting signal in vesicle-associated membrane proteins". J. Biol. Chem. 274 (52): 36876–82. doi:10.1074/jbc.274.52.36876. PMID 10601239.
* Paumet F, Le Mao J, Martin S, et al. (2000). "Soluble NSF attachment protein receptors (SNAREs) in RBL-2H3 mast cells: functional role of syntaxin 4 in exocytosis and identification of a vesicle-associated membrane protein 8-containing secretory compartment". J. Immunol. 164 (11): 5850–7. doi:10.4049/jimmunol.164.11.5850. PMID 10820264.
* Antonin W, Holroyd C, Tikkanen R, et al. (2000). "The R-SNARE Endobrevin/VAMP-8 Mediates Homotypic Fusion of Early Endosomes and Late Endosomes". Mol. Biol. Cell. 11 (10): 3289–98. doi:10.1091/mbc.11.10.3289. PMC 14992. PMID 11029036.
* Antonin W, Holroyd C, Fasshauer D, et al. (2001). "A SNARE complex mediating fusion of late endosomes defines conserved properties of SNARE structure and function". EMBO J. 19 (23): 6453–64. doi:10.1093/emboj/19.23.6453. PMC 305878. PMID 11101518.
* Nagamatsu S, Nakamichi Y, Watanabe T, et al. (2001). "Localization of cellubrevin-related peptide, endobrevin, in the early endosome in pancreatic beta cells and its physiological function in exo-endocytosis of secretory granules". J. Cell Sci. 114 (Pt 1): 219–227. doi:10.1242/jcs.114.1.219. PMID 11112705.
* Wade N, Bryant NJ, Connolly LM, et al. (2001). "Syntaxin 7 complexes with mouse Vps10p tail interactor 1b, syntaxin 6, vesicle-associated membrane protein (VAMP)8, and VAMP7 in b16 melanoma cells". J. Biol. Chem. 276 (23): 19820–7. doi:10.1074/jbc.M010838200. PMID 11278762.
* Antonin W, Fasshauer D, Becker S, et al. (2002). "Crystal structure of the endosomal SNARE complex reveals common structural principles of all SNAREs". Nat. Struct. Biol. 9 (2): 107–11. doi:10.1038/nsb746. hdl:11858/00-001M-0000-0012-F459-F. PMID 11786915. S2CID 17724790.
* Polgár J, Chung SH, Reed GL (2002). "Vesicle-associated membrane protein 3 (VAMP-3) and VAMP-8 are present in human platelets and are required for granule secretion". Blood. 100 (3): 1081–3. doi:10.1182/blood.V100.3.1081. PMID 12130530. S2CID 36597939.
* Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
* Imai A, Nashida T, Yoshie S, Shimomura H (2003). "Intracellular localisation of SNARE proteins in rat parotid acinar cells: SNARE complexes on the apical plasma membrane". Arch. Oral Biol. 48 (8): 597–604. doi:10.1016/S0003-9969(03)00116-X. PMID 12828989.
* Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
* Hillier LW, Graves TA, Fulton RS, et al. (2005). "Generation and annotation of the DNA sequences of human chromosomes 2 and 4". Nature. 434 (7034): 724–31. Bibcode:2005Natur.434..724H. doi:10.1038/nature03466. PMID 15815621.
* Brinkman JF, Ottenheim CP, de Jong LA, et al. (2006). "VAMP5 and VAMP8 are most likely not involved in primary open-angle glaucoma". Mol. Vis. 11: 582–6. PMID 16110299.
* Oishi Y, Arakawa T, Tanimura A, et al. (2007). "Role of VAMP-2, VAMP-7, and VAMP-8 in constitutive exocytosis from HSY cells". Histochem. Cell Biol. 125 (3): 273–81. doi:10.1007/s00418-005-0068-y. PMID 16195891. S2CID 10541252.
* Shiffman D, Rowland CM, Louie JZ, et al. (2006). "Gene variants of VAMP8 and HNRPUL1 are associated with early-onset myocardial infarction". Arterioscler. Thromb. Vasc. Biol. 26 (7): 1613–8. doi:10.1161/01.ATV.0000226543.77214.e4. PMID 16690874.

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