Flavonol synthase

In enzymology, a flavonol synthase (EC 1.14.11.23) is an enzyme that catalyzes the following chemical reaction :

dihydroflavonol + 2-oxoglutarate + O₂ ${\displaystyle \rightleftharpoons }$ a flavonol + succinate + CO₂ + H₂O

The 3 substrates of this enzyme are dihydroflavonol, 2-oxoglutarate, and O₂, whereas its 4 products are flavonol, succinate, CO₂, and H₂O.

This enzyme belongs to the family of oxidoreductases, specifically those acting on paired donors, with O₂ as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derived from O₂ with 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor. The systematic name of this enzyme class is dihydroflavonol,2-oxoglutarate:oxygen oxidoreductase. This enzyme participates in flavonoid biosynthesis.

• "Functional expression and mutational analysis of flavonol synthase from Citrus unshiu". Eur. J. Biochem. 269 (16): 4134–42. 2002. doi:10.1046/j.1432-1033.2002.03108.x. PMID 12180990. 
• "Flavonol synthase from Citrus unshiu is a bifunctional dioxygenase". Phytochemistry 62 (3): 287–92. 2003. doi:10.1016/S0031-9422(02)00567-8. PMID 12620339. 
• "Divergent evolution of flavonoid 2-oxoglutarate-dependent dioxygenases in parsley". FEBS Lett. 544 (1-3): 93–8. 2003. doi:10.1016/S0014-5793(03)00479-4. PMID 12782296. 
• CJ; Nakajima, J; Welford, RW; Yamazaki, M; Saito, K; Schofield, CJ (2004). "Mechanistic studies on three 2-oxoglutarate-dependent oxygenases of flavonoid biosynthesis: anthocyanidin synthase, flavonol synthase, and flavanone 3beta-hydroxylase". J. Biol. Chem. 279 (2): 1206–16. doi:10.1074/jbc.M309228200. PMID 14570878. 

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