Resolution (chromatography)

Example chromatogram showing signal as a function of retention time

In chromatography, resolution is a measure of the separation of two peaks of different retention time t in a chromatogram.^[1][2][3][4]

Expression

Chromatographic peak resolution is given by

[math]\displaystyle{ R_s = 2\cfrac{t_{R2}-t_{R1}}{w_{b1}+w_{b2}} }[/math]

where t_R is the retention time and w_b is the peak width at baseline. Here compound 1 elutes before compound 2.

If the peaks have the same width

[math]\displaystyle{ R_s = \cfrac{t_{R2}-t_{R1}}{w_b} }[/math].

Plate number

Two resolved peaks in a chromatogram

The theoretical plate height is given by

[math]\displaystyle{ H = \frac{L}{N} }[/math]

where L is the column length and N the number of theoretical plates.^[5] The relation between plate number and peak width at the base is given by

[math]\displaystyle{ N = 16 \cdot \left(\frac{t_R}{W_b}\right)^2 \, }[/math].

See also

• Image resolution
• Resolution (mass spectrometry)
• Van Deemter equation

References

External links

• IUPAC Nomenclature for Chromatography

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