Resolution (chromatography)

In chromatography, resolution is a measure of the separation of two peaks of different retention time t in a chromatogram.^[1][2][3][4]

Chromatographic peak resolution is given by

${\displaystyle R_s=2\frac{t_{R2}-t_{R1}}{w_{b1}+w_{b2}}}$

where t_R is the retention time and w_b is the peak width at baseline. Here compound 1 elutes before compound 2.

If the peaks have the same width

${\displaystyle R_s=\frac{t_{R2}-t_{R1}}{w_b}}$.

The theoretical plate height is given by

${\displaystyle H=\frac{L}{N}}$

where L is the column length and N the number of theoretical plates.^[5] The relation between plate number and peak width at the base is given by

${\displaystyle N=16\cdot {\left(\frac{t_R}{W_b}\right)}^2}$.

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• IUPAC Nomenclature for Chromatography

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