Bioinformatics tool gene transcriptions

From Wikidoc - Reading time: 9 min

Associate Editor(s)-in-Chief: Henry A. Hoff

Def. a "field of science in which biology, computer science, and information technology merge into a single discipline to analyse biological information using computers and statistical techniques"[1] is called bioinformatics.

"To understand the gene function and regulation patterns of CsSODs, sequences of 1000 bp upstream regions from the translation start site of each CsSOD were identified from [Cucumber Genome Initiative (CuGI, http://cucumber.genomics.org.cn/)] CuGI and were subsequently determined using PlantCARE. The results showed that 59 types of cis-acting regulatory elements were found in the promoter region of CsSODs and all CsSOD promoters contained typical TATA and CAAT boxes, which are essential elements of the promoters [...]."[2]

Human genes[edit | edit source]

"Briefly, the mRNA sequences were obtained from the NCBI Reference Sequence Database (RefSeq) for human SIRT2 gene. The reference mRNA sequences was submitted to BLAST for comparison with the human genomic DNA sequence in the human genome database."[3]

Human promoter occurrences[edit | edit source]

"After noting the orientation of the alignment, the appropriate 5-Kb genomic DNA sequence upstream from the transcription start point corresponding to the promoter region of the gene was isolated and analyzed using a web-based bioinformatics tool TESS at http://www.cbil.upenn.edu/cgi-bin/tess/tess. Because TESS results are model-based, the potential CArG sequence was verified by both using LALIGN (http://www.ch.embnet.org/software/LALIGN_form.html) and visual confirmation."[3]

Acknowledgements[edit | edit source]

The content on this page was first contributed by: Henry A. Hoff.

See also[edit | edit source]

References[edit | edit source]

  1. 201.129.67.142 (6 May 2005). "bioinformatics". San Francisco, California: Wikimedia Foundation, Inc. Retrieved 23 February 2021.
  2. Yong Zhou, Lifang Hu, Hao Wu, Lunwei Jiang, and Shiqiang Liu (20 July 2017). "Genome-Wide Identification and Transcriptional Expression Analysis of Cucumber Superoxide Dismutase (SOD) Family in Response to Various Abiotic Stresses". International Journal of Genomics. 2017: 7243973. doi:10.1155/2017/7243973. Retrieved 26 February 2021.
  3. 3.0 3.1 Xiaomin Zhang, Gohar Azhar, Jeanne Y. Wei (21 December 2017). "SIRT2 gene has a classic SRE element, is a downstream target of serum response factor and is likely activated during serum stimulation". PLOS One. 12 (12): e0190011. doi:10.1371/journal.pone.0190011. Retrieved 23 February 2021.

External links[edit | edit source]


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