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The James Preserve (also known as the Darwin James Preserve[1]) is a nature preserve located on Long Island in the village of Old Brookville in Nassau County, New York, and connected to Greenvale.
The 19.4-acre (7.9 ha) preserve was maintained by The Nature Conservancy[2] prior to being transferred to the North Shore Land Alliance in 2015.[1] People infer it was a former farm seeing as there are abandoned buildings, likely barns, with farming tools inside. It contains a swamp and a river with a bridge crossing over it. A trail within the preserve leads to Valentines Lane, which leads to another nature preserve, Louis C. Clark Sanctuary.