In The Blues Brothers, the band performs "Rawhide" in a local country western bar.
The song is played in a scene on An American Tail: Fievel Goes West, when the titular character is rolling through the desert inside a tumbleweed. The song is actually the cover reused from The Blues Brothers, mentioned above, performed by an uncredited Dan Aykroyd and John Belushi as various desert animals. However, the song was removed from the soundtrack's later releases due to copyright.
In My Favorite Martian, the sentient, wise-cracking metallic suit Zoot (voiced by an uncredited Wayne Knight) sings a verse of the song as he and fellow Martians Martin (Christopher Lloyd) and Neenert (Ray Walston) leave Earth on their spacecraft.
The song is parodied in the Histeria! episode "North America" as the Cowpie Song.
In A Little Curious, episode Long, Short, Roll, there is a segment with a song that is a parody of "Rawhide".
The jingle for the Rosen Motor Group used car dealership ("Rosen, Rosen, Rosen, Rosen, Rosen, Rosen") is based on the Rawhide theme.
A series of mid-1990s advertisements for the Walmart chain that focus on its rolling back prices use a song based on the Rawhide theme.
In the Star Trek tie-in novel How Much for Just the Planet? by John M. Ford, the play-acting Direidi barbarian followers of Queen Janeka sing a song whose lyrics (about the difficulties of being a barbarian horde in the 23rd century) parallel those of the "Rawhide" theme.