Thiazole synthase

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Thiazole synthase
Thiazole synthase
	Thiazole synthase oktamer, Saccharomyces cerevisiae
Identifiers
EC no.	2.8.1.10
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
PMC
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PMC	articles
PubMed	articles
NCBI	proteins

Thiazole synthase (EC 2.8.1.10, thiG (gene)) is an enzyme with systematic name 1-deoxy-D-xylulose 5-phosphate:thiol sulfurtransferase.^[1]^[2]^[3]^[4]^[5]^[6] This enzyme catalyses the following chemical reaction

1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-adenylate-[sulfur-carrier protein ThiS]

\rightleftharpoons

2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H₂O

H₂S can provide the sulfur in vitro.

References[edit]

^ Park JH, Dorrestein PC, Zhai H, Kinsland C, McLafferty FW, Begley TP (October 2003). "Biosynthesis of the thiazole moiety of thiamin pyrophosphate (vitamin B1)". Biochemistry. 42 (42): 12430–8. doi:10.1021/bi034902z. PMID 14567704.
^ Dorrestein PC, Zhai H, McLafferty FW, Begley TP (October 2004). "The biosynthesis of the thiazole phosphate moiety of thiamin: the sulfur transfer mediated by the sulfur carrier protein ThiS". Chemistry & Biology. 11 (10): 1373–81. doi:10.1016/j.chembiol.2004.08.009. PMID 15489164.
^ Dorrestein PC, Zhai H, Taylor SV, McLafferty FW, Begley TP (March 2004). "The biosynthesis of the thiazole phosphate moiety of thiamin (vitamin B1): the early steps catalyzed by thiazole synthase". Journal of the American Chemical Society. 126 (10): 3091–6. doi:10.1021/ja039616p. PMID 15012138.
^ Settembre EC, Dorrestein PC, Zhai H, Chatterjee A, McLafferty FW, Begley TP, Ealick SE (September 2004). "Thiamin biosynthesis in Bacillus subtilis: structure of the thiazole synthase/sulfur carrier protein complex". Biochemistry. 43 (37): 11647–57. doi:10.1021/bi0488911. PMID 15362849.
^ Hazra A, Chatterjee A, Begley TP (March 2009). "Biosynthesis of the thiamin thiazole in Bacillus subtilis: identification of the product of the thiazole synthase-catalyzed reaction". Journal of the American Chemical Society. 131 (9): 3225–9. doi:10.1021/ja806752h. PMC 2765510. PMID 19216519.
^ Hazra AB, Han Y, Chatterjee A, Zhang Y, Lai RY, Ealick SE, Begley TP (June 2011). "A missing enzyme in thiamin thiazole biosynthesis: identification of TenI as a thiazole tautomerase". Journal of the American Chemical Society. 133 (24): 9311–9. doi:10.1021/ja1110514. PMC 3116082. PMID 21534620.

External links[edit]

Thiazole+synthase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)

[1] Park JH, Dorrestein PC, Zhai H, Kinsland C, McLafferty FW, Begley TP (October 2003). "Biosynthesis of the thiazole moiety of thiamin pyrophosphate (vitamin B1)". Biochemistry. 42 (42): 12430–8. doi:10.1021/bi034902z. PMID 14567704.

[2] Dorrestein PC, Zhai H, McLafferty FW, Begley TP (October 2004). "The biosynthesis of the thiazole phosphate moiety of thiamin: the sulfur transfer mediated by the sulfur carrier protein ThiS". Chemistry & Biology. 11 (10): 1373–81. doi:10.1016/j.chembiol.2004.08.009. PMID 15489164.

[3] Dorrestein PC, Zhai H, Taylor SV, McLafferty FW, Begley TP (March 2004). "The biosynthesis of the thiazole phosphate moiety of thiamin (vitamin B1): the early steps catalyzed by thiazole synthase". Journal of the American Chemical Society. 126 (10): 3091–6. doi:10.1021/ja039616p. PMID 15012138.

[4] Settembre EC, Dorrestein PC, Zhai H, Chatterjee A, McLafferty FW, Begley TP, Ealick SE (September 2004). "Thiamin biosynthesis in Bacillus subtilis: structure of the thiazole synthase/sulfur carrier protein complex". Biochemistry. 43 (37): 11647–57. doi:10.1021/bi0488911. PMID 15362849.

[5] Hazra A, Chatterjee A, Begley TP (March 2009). "Biosynthesis of the thiamin thiazole in Bacillus subtilis: identification of the product of the thiazole synthase-catalyzed reaction". Journal of the American Chemical Society. 131 (9): 3225–9. doi:10.1021/ja806752h. PMC 2765510. PMID 19216519.

[6] Hazra AB, Han Y, Chatterjee A, Zhang Y, Lai RY, Ealick SE, Begley TP (June 2011). "A missing enzyme in thiamin thiazole biosynthesis: identification of TenI as a thiazole tautomerase". Journal of the American Chemical Society. 133 (24): 9311–9. doi:10.1021/ja1110514. PMC 3116082. PMID 21534620.

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v t e Transferases: sulfur-containing group (EC 2.8)
2.8.1: Sulfurtransferases	Thiosulfate sulfurtransferase Rhodanese 3-mercaptopyruvate sulfurtransferase thiosulfate—thiol sulfurtransferase tRNA sulfurtransferase thiosulfate—dithiol sulfurtransferase biotin synthase cysteine desulfurase
2.8.2: Sulfotransferases	Alcohol sulfotransferase Tyrosylprotein sulfotransferase Aryl sulfotransferase
2.8.3: CoA-transferases	Propionate CoA-transferase
2.8.4: Alkylthio	Coenzyme-B sulfoethylthiotransferase

v t e Enzymes
Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Diffusion-limited enzyme Coenzyme Cofactor Enzyme catalysis
Regulation	Allosteric regulation Cooperativity Enzyme inhibitor Enzyme activator
Classification	EC number Enzyme superfamily Enzyme family List of enzymes
Kinetics	Enzyme kinetics Eadie–Hofstee diagram Hanes–Woolf plot Lineweaver–Burk plot Michaelis–Menten kinetics
Types	EC1 Oxidoreductases (list) EC2 Transferases (list) EC3 Hydrolases (list) EC4 Lyases (list) EC5 Isomerases (list) EC6 Ligases (list) EC7 Translocases (list)