Liquid chromatography is an analytical method that uses selective absorption to separate the components of a mixture in solution. This method uses a moving solvent, a means of producing solvent motion (such as gravity or a pump), a means of sample introduction, a fractioning column, and a detector.
Improvements allow operation in three separation modes:
Examples of common solvents are hexane, isooctane, tetrahydrofuran, methyl ethyl ketone, acetone-chloroform, and toluene.
Examples of common packing materials are silica gel, polystyrene gel, ion exchange resins, alumina, and glass beads.
Categories: [Chemistry]