Straw (Cryogenic Storage)

A cryopreservation straw is a small storage device used for the cryogenic storage of liquid samples, often in a biobank or other collection of samples. Their most common application is for storage of sperm for in-vitro fertilization.

Ideally such straws should be made of a material that is chemically inert, biocompatible and have physical characteristics that make them resistant to ultra-low temperatures and pressures created by their storage conditions, resulting in the expansion of liquids and liquid nitrogen.^[1]

Use

Once the sample has been introduced into the straw, both extremities are thermally sealed using a specific device, usually supplied by the manufacturer of the consumable.^[2]

The straws are then stored within triangular or square visotubes, which in turn fit into cylindrical or square containers known as goblets. These are then organized into a matrix of the same within an ultra-low temperature freezer or nitrogen tank.

Characteristics

• specifically designed for storing biological materials at temperatures as low as -190 °C;
• stable when submitted to sudden low temperatures (snap freezing), when held at low temperatures for long periods of time (years ) or when taken through several freeze-thaw cycles; and
• as leak proof as possible even at the lowest cryogenic temperatures.^[2]

References

• Embryo and Sperm Freezing – Cryopreservation – Arizona
• Nonequilibrium cryopreservation of rabbit embryos using a modified (sealed) open pulled straw procedure | Animal Reproductive Biology and Embryology

External links

• Safety of vitrification. Embryo News. June 28, 2013.

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