Aniline Blue Ws

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Short description: Chemical compound
Water blue
Methyl blue

Aniline Blue WS, also called aniline blue, diphenylamine blue, China blue, or Soluble blue, is a mixture of methyl blue and water blue. It may also be either one of them.[1] It is a soluble dye used as a biological dye,[2] in fluorescence microscopy, appearing a yellow-green colour after excitation with violet light.[3] It is a mixture of the trisulfonates of triphenyl rosaniline and of diphenyl rosaniline.[4]

Aniline blue or its constituents are used to stain collagen, as the fibre stain in Masson's trichrome,[5] as well as to reveal callose structures in plant tissues.[6]

It can be used in the Mallory's connective tissue stain[5] and Gömöri trichrome stain. It is used in differential staining.

References

  1. "Stainsfile - Aniline blue WS". http://stainsfile.info/StainsFile/dyes/707.htm. 
  2. "Medical Definition of ANILINE BLUE". http://www.merriam-webster.com/medical/aniline+blue. 
  3. "Fluorescence Microscope Images". http://www.biologie.uni-hamburg.de/b-online/library/webb/BOT410/410Labs/Fluor-99/fluorescence_microscope_images.htm. 
  4. "aniline blue". http://medical-dictionary.thefreedictionary.com/aniline+blue. 
  5. 5.0 5.1 "Stainsfile - Water blue". http://stainsfile.info/StainsFile/dyes/42755.htm. 
  6. "Protocols - Staining with trypan blue and aniline blue - Felix Mauch's Group". http://commonweb.unifr.ch/biol/pub/mauchgroup/staining.html. 



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Categories: [Staining dyes] [Triarylmethane dyes]


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